2006 Vol. 24, No. 5
Display Method:
2006, (5): 264-267.
Abstract:
Objective: To study the effects of four flavonoids （fisetin, quercetin, apigenin, and phloretin） on production and ac-tions of fibrogenic cytokines. Methods: Mouse peritoneal macrophages were primed with calcimycin 10-6mol/L for 8h then elicited by lipopolysaccharides （LPS） 100g/L for 6h to induce fibrogenic cytokines.Proliferative and collagen stimulating activity in the macro-phage culture supernatants was determined by crystal violet staining assay and [3H]-proline incorporation assay using rat hepatic stel-late HSC-T6 cell.Transforming growth factorβ（TGFβ） activity was measured by [3H]-thymidine incorporation assay using Mv-1-Lu mink lung epithelial cell. Results: Fisetin, quercetin, apigenin, and phloretin （12.5～50μmol/L） concentration-dependently inhibit LPS-induced collagen stimulating activities and TGFβproduction whereas they did not inhibit proliferative activities released by macro-phages. Conclusion: The four flavonoids reducted fibrogenic cytokines production and blockade of their actions on hepatic stellate cells.
Objective: To study the effects of four flavonoids （fisetin, quercetin, apigenin, and phloretin） on production and ac-tions of fibrogenic cytokines. Methods: Mouse peritoneal macrophages were primed with calcimycin 10-6mol/L for 8h then elicited by lipopolysaccharides （LPS） 100g/L for 6h to induce fibrogenic cytokines.Proliferative and collagen stimulating activity in the macro-phage culture supernatants was determined by crystal violet staining assay and [3H]-proline incorporation assay using rat hepatic stel-late HSC-T6 cell.Transforming growth factorβ（TGFβ） activity was measured by [3H]-thymidine incorporation assay using Mv-1-Lu mink lung epithelial cell. Results: Fisetin, quercetin, apigenin, and phloretin （12.5～50μmol/L） concentration-dependently inhibit LPS-induced collagen stimulating activities and TGFβproduction whereas they did not inhibit proliferative activities released by macro-phages. Conclusion: The four flavonoids reducted fibrogenic cytokines production and blockade of their actions on hepatic stellate cells.
2006, (5): 267-269.
Abstract:
Objective: To determine the contents of diosgenin in Dioscorea nipponica Makino produced in differ-ent areas and find the best producing area. Methods: The contents of diosgenin in Dioscorea nipponica Makino were determine by a RP-HPLC method. Results: It shows that the content varies greatly in different producing areas.The content of diosgenin is higher in Henlongjiang, Neimeng, Hebei, among which the content in Heilongjiang is the hightest (1.49%). Conclusion: Heilongjiang can considered as the optimal producing area of diosgenin in Dioscorea nipponica Makino.
Objective: To determine the contents of diosgenin in Dioscorea nipponica Makino produced in differ-ent areas and find the best producing area. Methods: The contents of diosgenin in Dioscorea nipponica Makino were determine by a RP-HPLC method. Results: It shows that the content varies greatly in different producing areas.The content of diosgenin is higher in Henlongjiang, Neimeng, Hebei, among which the content in Heilongjiang is the hightest (1.49%). Conclusion: Heilongjiang can considered as the optimal producing area of diosgenin in Dioscorea nipponica Makino.
2006, (5): 270-271.
Abstract:
Objective: To develop a method for determination of arginine in human fibrinogen. Methods: The sample was mixed with alanine as the internal standard, derived by 2, 4-dinitrofluorobenzene.The mobil phase was composed of 50% acetonitrile-0.05 mol/L acetate buffer(35∶65).The UV detection wavelength was 362nm. Results: There was a good linear relationship with the range of 0.16～0.06mg/mL(r=0.9998), average recovery of arginine was 102.4%, RSD was 1.4%. Conclusion: This method is accu-rate, simple, cheap, and can be used for determination of arginine in human fibrinogen.
Objective: To develop a method for determination of arginine in human fibrinogen. Methods: The sample was mixed with alanine as the internal standard, derived by 2, 4-dinitrofluorobenzene.The mobil phase was composed of 50% acetonitrile-0.05 mol/L acetate buffer(35∶65).The UV detection wavelength was 362nm. Results: There was a good linear relationship with the range of 0.16～0.06mg/mL(r=0.9998), average recovery of arginine was 102.4%, RSD was 1.4%. Conclusion: This method is accu-rate, simple, cheap, and can be used for determination of arginine in human fibrinogen.
2006, (5): 271-274.
Abstract:
Objective: To compare the differences and advantages in the application of sort-based analysis and ABC inventory theo-ry in hospital pharmacy. Methods: Drug inventory data were collected from a teaching hoapital with 1000 beds.The data were calculat-ed through the application of ABC inventory theory and sort-based analysis. Results: A items were classified by ABC inventory theory, which had 215 items(17 percent of total items), 71.67 million units(46.41 percent of total inventory units) with 275.98 million yuan in whole cost (80.06 percent of total inventory costs);and the important items were classified by sort-based analysis which had 512 i-tems (40 percent of total items), 139 million units (90.01 percent of total inventory units) with 314.17 million yuan in whole cost (91.15 percent of total inventory costs). Conclusion: sort-based analysis can fetch up some shortages of ABC inventory theory in op-eration process, which should be used to improve the efficiency of inventory management in hospital pharmacy.
Objective: To compare the differences and advantages in the application of sort-based analysis and ABC inventory theo-ry in hospital pharmacy. Methods: Drug inventory data were collected from a teaching hoapital with 1000 beds.The data were calculat-ed through the application of ABC inventory theory and sort-based analysis. Results: A items were classified by ABC inventory theory, which had 215 items(17 percent of total items), 71.67 million units(46.41 percent of total inventory units) with 275.98 million yuan in whole cost (80.06 percent of total inventory costs);and the important items were classified by sort-based analysis which had 512 i-tems (40 percent of total items), 139 million units (90.01 percent of total inventory units) with 314.17 million yuan in whole cost (91.15 percent of total inventory costs). Conclusion: sort-based analysis can fetch up some shortages of ABC inventory theory in op-eration process, which should be used to improve the efficiency of inventory management in hospital pharmacy.
2006, (5): 277-280.
Abstract:
Objective: To investigate the present situation of antimicrobial usage in the patients who underwent surgical operation in a three-grade hospital. Methods: According to the designed table with unified contents and standard, a retrospective investigation was made in 97 operated patients who had been discharged from the hospital. Results: In the 97 patients who underwent surgical oper-ation, antibacterial use in 56 patients was rational, 41 patients had irrational antibacterial use.The irrational antibacterial combination use happened most frequently and the cases were 17, the other irrational aspects were antibacterial selection and indication, usage and dosage, preventive medication, the cases were 16, 16, 13 respectively;of all antimicrobials used, cephalosporins were used most fre-quently, penicilins were the second, then were aminoglycosides.These three kinds of antibiotics accounted for 72.32% of all antibiot-ics used. Conclusion: The results show that it is very important to strengthen and improve the management of the antimicrobial use in a normal and standard way in hospital.
Objective: To investigate the present situation of antimicrobial usage in the patients who underwent surgical operation in a three-grade hospital. Methods: According to the designed table with unified contents and standard, a retrospective investigation was made in 97 operated patients who had been discharged from the hospital. Results: In the 97 patients who underwent surgical oper-ation, antibacterial use in 56 patients was rational, 41 patients had irrational antibacterial use.The irrational antibacterial combination use happened most frequently and the cases were 17, the other irrational aspects were antibacterial selection and indication, usage and dosage, preventive medication, the cases were 16, 16, 13 respectively;of all antimicrobials used, cephalosporins were used most fre-quently, penicilins were the second, then were aminoglycosides.These three kinds of antibiotics accounted for 72.32% of all antibiot-ics used. Conclusion: The results show that it is very important to strengthen and improve the management of the antimicrobial use in a normal and standard way in hospital.
2006, (5): 281-283.
Abstract:
Objective: To improve the dissolution rate of sodium ferulate by preparing the dropping pill using the technology of solid dispersion. Methods: The dropping pill was prepared with PEG 6000 and poloxamer 188 as carriers.The dissolution rate of sodium fer-ulate was measured using the basket method as well as HPLC. Results: The higher the ratio of PEG 6000 vs sodium ferulate in the dropping pill was, the greater the dissolution rate of the drug was.The dropping pill prepared was rounded and homogeneous.With 0.1 mol/L hydrochloride solution, water and pH 6.8 phosphrate buffer solution as the media, the dissolution rate of sodium ferulate from dropping pill was significantly faster than that from the marketing tablet of sodium ferulate, respectively. Conclusion: Sodium ferulate dropping pill had excellent rapid release effect.
Objective: To improve the dissolution rate of sodium ferulate by preparing the dropping pill using the technology of solid dispersion. Methods: The dropping pill was prepared with PEG 6000 and poloxamer 188 as carriers.The dissolution rate of sodium fer-ulate was measured using the basket method as well as HPLC. Results: The higher the ratio of PEG 6000 vs sodium ferulate in the dropping pill was, the greater the dissolution rate of the drug was.The dropping pill prepared was rounded and homogeneous.With 0.1 mol/L hydrochloride solution, water and pH 6.8 phosphrate buffer solution as the media, the dissolution rate of sodium ferulate from dropping pill was significantly faster than that from the marketing tablet of sodium ferulate, respectively. Conclusion: Sodium ferulate dropping pill had excellent rapid release effect.
2006, (5): 283-285.
Abstract:
Objective: To prepare compound ofloxacin film and observe its clinical effect. Methods: Chitosan was used as the base material to make compound film which including drugs such as ofloxacin, tetracaine hydrochloride and so on.The quality control meth-od of the film was established and the clinical effect was observed. Results: The prepare technique is simple and the quality control is reliable.The effective rates of using the film to treat recurrent oral ulcer was 98.8%. Conclusion: Compound ofloxacin film can be pr-pared easily.The film is reliable curative effect and salty.
Objective: To prepare compound ofloxacin film and observe its clinical effect. Methods: Chitosan was used as the base material to make compound film which including drugs such as ofloxacin, tetracaine hydrochloride and so on.The quality control meth-od of the film was established and the clinical effect was observed. Results: The prepare technique is simple and the quality control is reliable.The effective rates of using the film to treat recurrent oral ulcer was 98.8%. Conclusion: Compound ofloxacin film can be pr-pared easily.The film is reliable curative effect and salty.
2006, (5): 285-288.
Abstract:
Objective: To prepare ofloxacin gargle and observe its stability. Methods: Referring to relative literature, the formula and preparing procedure of ofloxacin gargle were established, the stability of the gargle was observed by using initial average rate meth-od. Results: The linear relationship was good in the initial average rate method.The expective effective time at room temperature (t0.9) was was more than 310 days.Ofloxacin's recycle rate was 99.6%. Conclusion: The preparation has good stability, and can be used as clinical therapeutic medicine.
Objective: To prepare ofloxacin gargle and observe its stability. Methods: Referring to relative literature, the formula and preparing procedure of ofloxacin gargle were established, the stability of the gargle was observed by using initial average rate meth-od. Results: The linear relationship was good in the initial average rate method.The expective effective time at room temperature (t0.9) was was more than 310 days.Ofloxacin's recycle rate was 99.6%. Conclusion: The preparation has good stability, and can be used as clinical therapeutic medicine.
2006, (5): 289-291.
Abstract:
Objective: Separation of enantiomer of amlodipine by CE and HPLC. Methods: Different separation conditions such as buffer pH, the concentration of the chiral selector, buffer concentrations, temperature and applied voltage, etc.were studied and op-timized.The separation was finally conducted in 100mM orthophosphoric acid-Tris buffer (pH3.0) with 1.0% (w/w) HP-β-CD.The applied voltage was 16kV and the temperature 15℃. Results: Both CE and HPLC could give enantiomer of amlodipine resolution for adrenaline. Conclusion: The method proved to be specific and accurate
Objective: Separation of enantiomer of amlodipine by CE and HPLC. Methods: Different separation conditions such as buffer pH, the concentration of the chiral selector, buffer concentrations, temperature and applied voltage, etc.were studied and op-timized.The separation was finally conducted in 100mM orthophosphoric acid-Tris buffer (pH3.0) with 1.0% (w/w) HP-β-CD.The applied voltage was 16kV and the temperature 15℃. Results: Both CE and HPLC could give enantiomer of amlodipine resolution for adrenaline. Conclusion: The method proved to be specific and accurate
2006, (5): 291-293.
Abstract:
Objective: To observe the chemical stability of seven common injections mixed with ciprofloxacin lactate at room tem-perature for 8 hours. Methods: The appearance of mixed injection was observed and pH was tested.The convolution spectrometry was used.The results were described quantitatively by subtract spectrometry value. Results: The subtract spectrometry values of ciprofloxa-cin mixed with six infusions were less than 10%.The subtract spectrometry values of ciprofloxacin lactate mixed with sodium lactate in-jection were 1.57%, 31.76%, 26.46%, 22.85% in 2～8hours. Conclusion: At room temperature for 8 hours ciprofloxacin mixed with six infusions can be used, and ciprofloxacin lactate mixed with sodium lactate injection can't be used.
Objective: To observe the chemical stability of seven common injections mixed with ciprofloxacin lactate at room tem-perature for 8 hours. Methods: The appearance of mixed injection was observed and pH was tested.The convolution spectrometry was used.The results were described quantitatively by subtract spectrometry value. Results: The subtract spectrometry values of ciprofloxa-cin mixed with six infusions were less than 10%.The subtract spectrometry values of ciprofloxacin lactate mixed with sodium lactate in-jection were 1.57%, 31.76%, 26.46%, 22.85% in 2～8hours. Conclusion: At room temperature for 8 hours ciprofloxacin mixed with six infusions can be used, and ciprofloxacin lactate mixed with sodium lactate injection can't be used.
2006, (5): 293-295.
Abstract:
Objective: To establish the determination of hesperidin in Bainiankang oral liquid. Methods: The determination was car-ried out by HPLC with a YWG C18（150mm×4.6mm, 5μm）column, acetonitrile-0.25% water（20:80）as mobile phase, detection wavelength was at 285nm, flow rate was 1.0 mL/min. Results: Hesperidin showed a good linear relationship at the range of 5～50mg/L, r=0.999 6.The average recovery was 100.92% and RSD was 1.78%. Conclusion: The method is sensitive, accurate, re-producible, specific and can be used for quality control of Bainiankang oral liquid.
Objective: To establish the determination of hesperidin in Bainiankang oral liquid. Methods: The determination was car-ried out by HPLC with a YWG C18（150mm×4.6mm, 5μm）column, acetonitrile-0.25% water（20:80）as mobile phase, detection wavelength was at 285nm, flow rate was 1.0 mL/min. Results: Hesperidin showed a good linear relationship at the range of 5～50mg/L, r=0.999 6.The average recovery was 100.92% and RSD was 1.78%. Conclusion: The method is sensitive, accurate, re-producible, specific and can be used for quality control of Bainiankang oral liquid.
2006, (5): 299-302.
Abstract:
Objective: To establish a convenient and speedy reporting system on intranet for Adverse Drug Reaction. Methods: M-ter the web server was set up, the active server page was edited by VBScript.And the connection of ADR information between the web server and the workstation in clinic was achieved. Results: A system of Adverse Drug Reaction reports was established.Only inputting in workstation will add the information of Adverse Drug Reaction in the database in server quickly and safely. Conclusion: It is vera-cious, simple and rapid to save and share the information of ADR by the reporting system.
Objective: To establish a convenient and speedy reporting system on intranet for Adverse Drug Reaction. Methods: M-ter the web server was set up, the active server page was edited by VBScript.And the connection of ADR information between the web server and the workstation in clinic was achieved. Results: A system of Adverse Drug Reaction reports was established.Only inputting in workstation will add the information of Adverse Drug Reaction in the database in server quickly and safely. Conclusion: It is vera-cious, simple and rapid to save and share the information of ADR by the reporting system.
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