夏枯草消瘤合剂对Lewis肺癌小鼠的药效作用

马明华; 汪晓河; 戴媛媛; 顾小燕; 吴铁军; 年华

doi:10.3969/j.issn.1006-0111.201812070

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夏枯草消瘤合剂对Lewis肺癌小鼠的药效作用

马明华, 汪晓河, 戴媛媛, 顾小燕, 吴铁军, 年华

文章导航 > 药学实践与服务 > 2020 > 38(1): 57-62

马明华, 汪晓河, 戴媛媛, 顾小燕, 吴铁军, 年华. 夏枯草消瘤合剂对Lewis肺癌小鼠的药效作用[J]. 药学实践与服务, 2020, 38(1): 57-62. doi: 10.3969/j.issn.1006-0111.201812070

引用本文:

MA Minghua, WANG Xiaohe, DAI Yuanyuan, GU Xiaoyan, WU Tiejun, NIAN Hua. Therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(1): 57-62. doi: 10.3969/j.issn.1006-0111.201812070

Citation:

MA Minghua, WANG Xiaohe, DAI Yuanyuan, GU Xiaoyan, WU Tiejun, NIAN Hua. Therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(1): 57-62. doi: 10.3969/j.issn.1006-0111.201812070

夏枯草消瘤合剂对Lewis肺癌小鼠的药效作用

doi: 10.3969/j.issn.1006-0111.201812070

1.
上海市杨浦区中心医院药剂科, 上海 200090
2.
上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437

基金项目: 上海中医药大学高峰高原学科2018年项目（30304114334）；中药炮制传承基地项目（30304118220）；上海市科学技术委员会科研计划项目（16401902100）；上海中医药大学预算内项目（18LK047）

Therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice

1.
Pharmaceutical Center of Yangpu District Central Hospital, Shanghai 200090, China
2.
Pharmaceutical Center of Yueyang Hospital, Shanghai University of TCM, Shanghai 200437, China

摘要: 目的探讨夏枯草消瘤合剂对Lewis肺癌小鼠的治疗作用。方法采用C₅₇BL/6小鼠Lewis肺癌移植瘤模型，将30只小鼠随机分为3组：模型对照组、夏枯草消瘤合剂组（M组）、顺铂组（DDP组），连续用药14 d。通过对Lewis肺癌小鼠的大体观察，测定肿瘤大小，HE染色方法测定肿瘤病理组织学变化，免疫组化方法测定肿瘤组织的细胞周期蛋白D1（CyclinD1）和P16的表达情况。结果模型对照组瘤体质量最重，与其他组比较差异均有统计学意义（P<0.05）；M组可在一定程度上改善小鼠的生存状态及生活质量；各组小鼠的肿瘤生长曲线及HE染色结果显示：M组可抑制肿瘤细胞的生长，且对正常细胞有保护作用；M组、DDP组均可显著降低CyclinD1阳性表达（P<0.01），但是M组对于提高P16阳性表达的效果不显著。结论夏枯草消瘤合剂具有较好的抑制肺癌生长的作用。
- 中医药 /
- 夏枯草消瘤合剂 /
- Lewis肺癌 /
- CyclinD1 /
- P16
Abstract: Objective To investigate the therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice. Methods 30 mice with C₅₇BL/6 mouse Lewis lung cancer xenograft model were randomly divided into three groups:model control group, Xiakucao Xiaoliu mixture group (M group), cisplatin group (DDP group). M group and DDP group were administered continuously for 14 days. Through the general observation of Lewis lung cancer mice, tumor size was determined, HE staining method was used to determine the histopathological changes of tumors, and the expression of CyclinD1 and P16 in tumor tissues was determined by immunohistochemistry. Results The tumor weight of the model control group was the heaviest, and the difference was statistically significant compared with other groups. (P<0.05). Survival state and quality of life of mice had been improved to some extent in M group. The results of tumor growth curve and HE staining in each group of mice showed that the growth of tumor cells had been inhibited and normal cells had been protected. The positive expression of CyclinD1 was significantly decreased in M group and DDP group (P<0.01), but the effect of M group on the improvement of P16 positive expression was not significant. Conclusion Xiakucao Xiaoliu mixture had a good effect on inhibiting lung tumor growth.
- Chinese Medicine /
- Xiakucao Xiaoliu mixture /
- Lewis lung cancer /
- CyclinD1 /
- P16

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夏枯草消瘤合剂对Lewis肺癌小鼠的药效作用

doi: 10.3969/j.issn.1006-0111.201812070

1. 上海市杨浦区中心医院药剂科, 上海 200090

2. 上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437

收稿日期: 2018-12-24
修回日期: 2019-09-22

关键词:

中医药 /

夏枯草消瘤合剂 /

Lewis肺癌 /

CyclinD1 /

P16

摘要: 目的探讨夏枯草消瘤合剂对Lewis肺癌小鼠的治疗作用。方法采用C₅₇BL/6小鼠Lewis肺癌移植瘤模型，将30只小鼠随机分为3组：模型对照组、夏枯草消瘤合剂组（M组）、顺铂组（DDP组），连续用药14 d。通过对Lewis肺癌小鼠的大体观察，测定肿瘤大小，HE染色方法测定肿瘤病理组织学变化，免疫组化方法测定肿瘤组织的细胞周期蛋白D1（CyclinD1）和P16的表达情况。结果模型对照组瘤体质量最重，与其他组比较差异均有统计学意义（P<0.05）；M组可在一定程度上改善小鼠的生存状态及生活质量；各组小鼠的肿瘤生长曲线及HE染色结果显示：M组可抑制肿瘤细胞的生长，且对正常细胞有保护作用；M组、DDP组均可显著降低CyclinD1阳性表达（P<0.01），但是M组对于提高P16阳性表达的效果不显著。结论夏枯草消瘤合剂具有较好的抑制肺癌生长的作用。

English Abstract

Therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice

1. Pharmaceutical Center of Yangpu District Central Hospital, Shanghai 200090, China

2. Pharmaceutical Center of Yueyang Hospital, Shanghai University of TCM, Shanghai 200437, China

Received Date: 2018-12-24
Rev Recd Date: 2019-09-22

Keywords:

Abstract: Objective To investigate the therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice. Methods 30 mice with C₅₇BL/6 mouse Lewis lung cancer xenograft model were randomly divided into three groups:model control group, Xiakucao Xiaoliu mixture group (M group), cisplatin group (DDP group). M group and DDP group were administered continuously for 14 days. Through the general observation of Lewis lung cancer mice, tumor size was determined, HE staining method was used to determine the histopathological changes of tumors, and the expression of CyclinD1 and P16 in tumor tissues was determined by immunohistochemistry. Results The tumor weight of the model control group was the heaviest, and the difference was statistically significant compared with other groups. (P<0.05). Survival state and quality of life of mice had been improved to some extent in M group. The results of tumor growth curve and HE staining in each group of mice showed that the growth of tumor cells had been inhibited and normal cells had been protected. The positive expression of CyclinD1 was significantly decreased in M group and DDP group (P<0.01), but the effect of M group on the improvement of P16 positive expression was not significant. Conclusion Xiakucao Xiaoliu mixture had a good effect on inhibiting lung tumor growth.