舒肝安乐宁浸膏对小鼠急性酒精性肝损伤保护作用的研究
Protective effect of Shugananlening extract on acute alcohol hepatic injury in mice
-
摘要: 目的 研究舒肝安乐宁浸膏对小鼠急性酒精性肝损伤的保护作用。 方法 以白酒灌胃法建立小鼠急性酒精性肝损伤模型,观察血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、肝组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、甘油三酯(TG)指标的变化,并进行病理组织学检测。 结果 各剂量舒肝安乐宁浸膏能显著降低小鼠血清ALT、AST活性和肝组织MDA、TG含量(P<0.05或P<0.01),提高肝组织SOD活性,减轻肝脏的病理损伤。 结论 舒肝安乐宁浸膏对急性酒精性肝损伤有保护作用。其作用机制可能为减少自由基的产生,抑制脂质过氧化反应,减少脂肪在肝脏的沉积。Abstract: Objective To study the protective effect of Shugananlening extract on acute alcoholic hepatic injury in mice. Methods The model of hepatic injury was prepared by intragastric infusion of alcohol, the change of alanine transaminase (ALT), aspartate transaminase (AST) in serum and liver superoxide dismutase (SOD), malondialdehyde (MDA) and triglyceride (TG) were observed. HE staining was used to observe the pathological changes in liver. Results The level of ALT, AST, TG and MDA were significantly reduced and the activity of SOD was enhanced by different doses of Shugananlening Extract. Furthermore, the pathological injury was also significantly abated by high-dose and middle-dose of Shugananlening Extract. Conclusion Shugananlening extract had a good protective effect on acute alcoholic hepatic injury in mice. The mechanisms probably contributed to the decrease of lipid peroxide and fatty sediment in liver.
-
Key words:
- Shugananlening extract /
- alcohol /
- hepatic injury
-
[1] 夏爱军,韦少宣,蔡文娥,等. 舒肝安乐宁浸膏对肝纤维化大鼠肝脏组织的影响[J].解放军药学学报, 2009, 25(3): 196. [2] 赵 敏,黄俊明,谭剑斌,等. 小鼠急性酒精性肝损伤模型的动态研究[J]. 毒理学杂志,2001, 27(6) :378. [3] 赵 敏,池莉平,王凤岩,等. 小鼠急性酒精性肝损伤模型的建立及应用[J],华南预防医学,2005,31(1):14. [4] Walch K, Alexander G. Alcoholic liver disease[J]. Postgrad Med J,2000, 76(895): 280. [5] 杜施霖,迟宝荣.龙牙葱木皂苷对大鼠酒精性肝病的防治作用[J].吉林大学学报,2005,31(1) : 64. [6] 王金萍,曾 明.可舒胶囊对小鼠酒精性肝损伤的影响[J].中国实验方剂学杂志,2004,10(6) : 64. [7] 裴凌鹏,金宗濂. 葛根黄酮改善小鼠抗氧化功能的研究[J]. 营养学报,2005,26(6): 505. [8] 于淑娥. 鳖甲软肝片抗肝纤维化的疗效观察[J]. 现代中西医结合杂志, 2007, 19(16): 2685. [9] 秦华珍,郑作文,邓家刚,等. 广西桂郁金对小鼠急性肝损伤的保护作用[J]. 广西中医学院学报, 2008, 11(1) : 1. [10] 孙玉凤,冯志杰,孙泽明,等. 黄芪抗肝纤维化的实验研究[J]. 河北中医药学报, 2008, 23(1): 9. [11] 陈纯馨,陈 忻,刘爱文,等. 绞股蓝抗自由基成分的提取和性能测定[J]. 食品科学, 2008, 29(9): 239 [12] Syamasundar KV, Singh B, Thakur RS, et al. Antihepato toxic principles of Phyllantus niruri herbs[J]. Ethnoparmacol, 1985, 14(1): 41. [13] 玉顺子. 叶下珠及其制剂药理作用及临床应用研究进展[J]. 中国药业. 2010, 19(7): 87. [14] 丘 辉. 黄芪多糖药理作用的研究进展[J]. 牡丹江医学院学报, 2011, 32(1): 60. [15] 黄雪萍. 绞股蓝总苷与辛伐他汀治疗原发性高脂血症的疗效比较[J]. 中国药业,2006,15(6): 46. [16] 于 新,杜志坚,陈悦娇,等. 白花蛇舌草提取物抗氧化作用的研究[J]. 食品与发酵工业,2002,28(3): 10. -
点击查看大图
计量
- 文章访问数: 2453
- HTML全文浏览量: 194
- PDF下载量: 491
- 被引次数: 0
下载:
