聚氨基酯作为基因载体的研究进展

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聚氨基酯作为基因载体的研究进展

王江峰, 鲁莹, 钟延强

文章导航 > 药学实践与服务 > 2011 > 29(3): 165-168

王江峰, 鲁莹, 钟延强. 聚氨基酯作为基因载体的研究进展[J]. 药学实践与服务, 2011, 29(3): 165-168.

引用本文:

王江峰, 鲁莹, 钟延强. 聚氨基酯作为基因载体的研究进展[J]. 药学实践与服务, 2011, 29(3): 165-168.

WANG Jiang-feng, LU Ying, ZHONG Yan-qiang. Research on poly amino ester as the gene vector[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(3): 165-168.

Citation:

WANG Jiang-feng, LU Ying, ZHONG Yan-qiang. Research on poly amino ester as the gene vector[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(3): 165-168.

聚氨基酯作为基因载体的研究进展

第二军医大学药剂学教研室,上海 200433

Research on poly amino ester as the gene vector

Department of pharmaceutical, Second Military Medical University, Shanghai 200433, China

摘要: 随着基因治疗研究的不断深入,寻求一种高效、安全、靶向表达的载体已成为了基因治疗的核心问题之一。聚氨基酯(poly amino ester,PAE)作为一种新型的阳离子聚合物基因载体,具有原料廉价、合成简单、结构多样、可降解、细胞毒性低和转染效率高等优点,已越来越受到人们的关注。本文将从聚氨基酯的合成途径、理化性质、聚氨基酯/DNA纳米粒的制备及聚氨基酯/DNA纳米粒转染效率和影响因素等方面详细阐述了近年来聚氨基酯作为基因载体的研究进展。
- 聚氨基酯 /
- 非病毒基因载体 /
- 阳离子聚合物
Abstract: With the deepening research of gene therapy, to find an efficient, safe, targeted expression gene vector had become one of the core issues of gene therapy. Poly amino ester (PAE) as a new type of cationic polymer gene carriers, with cheap raw materials, simple synthesis, diversity structure, biodegradability, low toxicity and high efficiency transfection, which had attracted lots of researchers' focus. Recent research of PAE as a gene vector from the poly amino ester synthesis pathway, physical and chemical properties, properties of poly amino ester / DNA nano-particles and transfection efficiency of polyamino ester/DNA nano-particles were summarized in this paper.
- poly amino esters /
- non-viral gene vector /
- cationic polymer

[1]	Sun JY, Anand-Jawa V, Chatterjee S, et al. Immune responses to adeno-associated virus and its recombinant vectors[J]. Gene Ther, 2003,10: 964.
[2]	Gore ME. Adverse effects of gene therapy: gene therapy can cause leukaemia: no shock, mild horror but a probe[J]. Gene Ther, 2003, 10: 4.
[3]	Boussif O, Lezoualc'h F, Zanta, et al. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine[J]. Proc Natl Acad Sci, 1995, 92: 7297.
[4]	Luo D, Saltzman WM. Synthetic DNA delivery systems[J]. NatBiotechnol, 2000, 18 (1):33.
[5]	Lemkine GF, Demeneix BA. Polyethylenimines for in vivo gene delivery[J]. Curr Opin Mol Ther, 2001, 3: 178.
[6]	Danusso F, Ferruti P. Synthesis of tertiary amine polymers[J]. Polymer, 1970, 11: 88.
[7]	Lynn DM, Robert Langer. Degradable poly(β-amino esters): synthesis, characterization, and self-assembly with plasmid DNA[J]. Am Chem Soc, 2000, 122:10761.
[8]	Lynn DM, Anderson DG, Putnam D, et al. Accelerated discovery of synthetic transfection vectors: parallel synthesis and screening of a degradable polymer library[J]. Am Chem Soc, 2001, 123: 8155.
[9]	Akin Akinc, Anderson DG, Lynn DM, et al. Synthesis of poly(β-amino ester)s optimized for highly effective gene delivery[J]. Bioconjugate Chem, 2003, 14: 979.
[10]	Anderson DG, Lynn DM, and Robert Langer. Semi-automated synthesis and screening of a large library of degradable cationic polymers for gene delivery[J]. Angew Chem Int Ed, 2003, 42: 3153 .
[11]	Zhong Zhiyuan , Song Yan , Johan FJ, et al. A versatile family of degradable non-viral gene carriers based on hyperbranched poly(ester amine)s[J]. Journal of Controlled Release, 2005,109: 317.
[12]	Tae-il Kim, Hyo Jung Seo, Joon Sig Choi, et al. Synthesis of biodegradable cross-linked poly(β-amino ester) for gene delivery and its modification, inducing enhanced transfection efficiency and stepwise degradation[J]. Bioconjugate Chem. 2005, 16:1140.
[13]	Hyun JK, Min SK, Joon SC, et al. Synthesis and characterization of poly (amino ester) for slow biodegradable gene delivery vector[J]. Bioorganic & Medicinal Chemistry, 2007, 15: 1708.
[14]	Liu G, Molas M, Grossmann GA, et al. Biological properties of poly-L-lysine-DNA complexes generated by cooperative binding of the polycation[J]. Biol Chem 2001, 276: 34379.
[15]	Ogris M, Steinlein P, Carotta S, et al. DNA/polyethylenimine transfection particles: influence of ligands, polymer size, and PEGylation on internalization and gene expression[J]. AAPS PharmSci. 2001, 3: 21.
[16]	Anderson DG, Akinc A, Hossain N, et al. Structure/property studies of polymeric gene delivery using a library of poly(beta-amino esters) [J]. Mol Ther, 2005, 11: 426.
[17]	Guo W, Lee RJ. Efficient gene delivery via non-covalent complexes of folic acid and polyethylenimine[J]. Controlled Release 2001, 77: 131.
[18]	Green J, Shi J, Chiu E, et al. Biodegradable polymeric vectors for gene delivery to human endothelial cells[J]. Bioconjugate Chem, 2006, 1: 1162.
[19]	韦卫中.阳离子聚合物纳米基因载体的研究进展[J].医学研究生学报,2004,17(4):335.
[20]	Akinc A, Langer R. Measuring the pH environment of DNA delivered using nonviral vectors: implications for lysosomal trafficking[J]. Biotechnol Bioeng, 2002, 78:503.
[21]	Schaffer DV, Fidelman NA, Dan N, et al. Vector unpacking as a potential barrier for receptor-mediated polyplex gene delivery[J]. Biotechnol Bioeng, 2000, 67: 598.
[22]	Choosakoonkriang S, Lobo BA, Koe GS, et al. Biophysical characterization of PEI/DNA complexes[J]. Pharm Sci, 2003, 92:1710.
[23]	Zugates GT, Nathan CT, Andreas Zumbuehl, et al. Gene delivery properties of end-modified poly(beta-amino ester)s[J]. Bioconjugate Chem. 2007, 18: 1887.
[24]	Zugates G, Anderson D, Little S, et al. Synthesis of poly(beta amino ester)s with thiol-reactive side chains for DNA delivery[J]. Am Chem Soc, 2006, 128: 12726.
[25]	Green JJ, Chiu E, Leshchiner ES, et al. Electrostatic ligand coatings of nanoparticles enable ligand-specific gene delivery to human primary cells[J]. Nano Lett, 2007, 7: 874.

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聚氨基酯作为基因载体的研究进展

第二军医大学药剂学教研室,上海 200433

收稿日期: 2010-08-02
修回日期: 2010-10-12

关键词:

非病毒基因载体 /

阳离子聚合物

摘要: 随着基因治疗研究的不断深入,寻求一种高效、安全、靶向表达的载体已成为了基因治疗的核心问题之一。聚氨基酯(poly amino ester,PAE)作为一种新型的阳离子聚合物基因载体,具有原料廉价、合成简单、结构多样、可降解、细胞毒性低和转染效率高等优点,已越来越受到人们的关注。本文将从聚氨基酯的合成途径、理化性质、聚氨基酯/DNA纳米粒的制备及聚氨基酯/DNA纳米粒转染效率和影响因素等方面详细阐述了近年来聚氨基酯作为基因载体的研究进展。

English Abstract

王江峰, 鲁莹, 钟延强. 聚氨基酯作为基因载体的研究进展[J]. 药学实践与服务, 2011, 29(3): 165-168.

引用本文:

王江峰, 鲁莹, 钟延强. 聚氨基酯作为基因载体的研究进展[J]. 药学实践与服务, 2011, 29(3): 165-168.

WANG Jiang-feng, LU Ying, ZHONG Yan-qiang. Research on poly amino ester as the gene vector[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(3): 165-168.

Citation:

WANG Jiang-feng, LU Ying, ZHONG Yan-qiang. Research on poly amino ester as the gene vector[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(3): 165-168.

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