大孔吸附树脂富集藏药镰形棘豆总黄酮的工艺研究
Study on enriching technic process of total flavones from Herba Oxytropis Falcatae by macroporous resin
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摘要: 目的: 建立镰形棘豆总黄酮的大孔吸附树脂富集工艺。 方法: 二氯氧锆比色法测定样品中总黄酮苷含量,考察D-101大孔吸附树脂富集镰形棘豆水提取物中总黄酮的动态吸附能力,解吸附溶媒浓度及体积,以及树脂的重复利用能力等。 结果: 富集后总黄酮苷含量达到26%,接近富集前的5倍。 结论: D-101型大孔吸附树脂对镰形棘豆水提物中总黄酮成分有较好的吸附能力,60%乙醇溶液解吸附快速有效,树脂重复利用度高。Abstract: Objective: To establish an enriching technic process of total flavones from Herba Oxytropis Falcatae by macroporous resin. Methods: Zirconium oxychloride（ZrOCl2）chromatometry was used to determine the content of total flavones in samples.Dynamic state adsorbability,concentration and volume of adsorption stripping solvent,and the re-utilization of D-101 macroporous resin were studied. Results: The content of total flavones in enrichment is 26%,which was nearly five times of that before enriched. Conclusion: Chromatometry with zirconium oxychloride as developer is simple and credible.D-101 macroporous resin showed better adsorbability of flavones from the aqueous extract of Herba Oxytropis Falcatae with high re-availability,and 60%alcohol could be used as adsorption stripping solvent because it works quickly and efficiently.
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