膜分离技术与传统工艺对六味地黄活性多糖富集效果的比较
Effect analysis of the concentration of Liuwei Dihuang polysaccharides by the membrane separation technique and the traditional method
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摘要: 目的:比较分析膜分离技术与传统工艺对六味地黄多糖活性成分的富集效果。方法:采用纳滤膜和10万分子量的超滤膜对两种工艺制得的六味地黄方药液进行分段富集,建立硫酸-蒽酮法测定各分段溶液的含糖量。结果:采用膜分离技术制成的滴心丸中活性多糖含量为经传统工艺制得的胶囊的6.5倍。结论:在富集六味地黄活性多糖方面,膜过滤工艺较传统工艺具有较大的优势。Abstract: Objective :To compare the effect between the membrane separation technique and the traditional method being used to concentrate polysaccharides from Liuwei Dihuang formula. Methods : The extract liquid of Liuwei Dihuang formula was divided into several levels and segments using nanofiltration and ultrafiltration of 10 000Mr.The polysaccharides in every level's extracted liquid were quantitate by sulfuric acid-anthrone colorimetric method at detecting wavelength 625 nm. Results : The polysaccharides in the production made by membrane separation technique were 6.5 times as much as that in the production made by the traditional method. Conclusion : The membrane separation technique was superior in concentration polysaccharides compared with the traditional method.
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