Carbazole method for simultaneous determination of sodium hyaluronate and chondroitin sulfate

Key words: 
• carbazole method /
• sodium hyaluronate /
• chondroitin sulfate /
• cetylpyridinium chloride /
• determination

Abstract: Objective :To establish a carbazole method for the simultaneous determination of sodium hyaluronate(SH) and chondroitin sulfate(CS). Methods : By regulating acidity or alkalinity and ionic strength with sodium acetate,CS was selectively precipitated with cetylpyridinium chloride(CPC),whereas SH was not.So SH and CS were separated. Results :The sample pretreatment conditions were as follows:Measuring 10 mL mixture of SH and CS accurately,adding 1.6 g sodium acetate and 10 mL CPC of 12.5 mmol/L,standing for 1 h after stirring thoroughly,then,the solution was filtered with 20 μm filter membrane and the filtrate was used to determine the content of SH;the precipitates on the filter membrane were resolved with 1.4 mol/L NaCl solution by ultrasonic treatment for the determination of CS.The average recoveries of SH and CS were 101.2% and 99.4% with RSD of 1.05% and 0.96%,respectively. Conclusion :This method can be used for the simultaneous determination of SH and CS without interference with each other.