One step assay for five components in Xianlinggubao capsule by HPLC method

CHEN Zongliang; L&#220; Xiaoxia; CHEN Guiqian; CHEN Jie

doi:10.3969/j.issn.1006-0111.2017.05.014

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Objective To assay Icariine, Epimedin C, asperosaponin Ⅵ, psoralen and angelicin in Xianlinggubao capsules via multi-wavelength HPLC method. Methods Separation was carried out on Welch Ultimate^® XB-C₁₈ column. The mobile phase was acetonitrile-water system and a linear gradient elution was used. The column temperature was 30 ℃. The detection wavelength for Icariine, Epimedin C,asperosaponin Ⅵ was set at 212 nm, psoralen and angelicin at 246 nm. Results Five components reached baseline separation, the linearity was good when sample size was in the range of 0.008 2-0.328 μg for Icariine(r=0.999 5), 0.055 6-2.224 μg for Epimedin C (r=0.999 6), 0.144 1-5.764 μg for asperosaponin Ⅵ(r=0.999 6), 0.005 4-0.215 2 μg for psoralen(r=0.998 0), 0.006 6-0.265 6 μg for angelicin(r=0.998 5). The average recoveries were 97.59%, 98.58%, 98.11%, 97.86%, 98.22% respectively. The RSDs of recovery were all less than 2.0%. Conclusion This method is simple, accurate, with good separation, high sensitivity for the assay of multiple components in Xianlinggubao capsule.

One step assay for five components in Xianlinggubao capsule by HPLC method

1. Jinhua Institute for Food and Drug Control, Jinhua 321000, China

2. Jinhua Jindong City Food and Drug Administration, Jinhua 321000, China

Received Date: 2016-06-28

Rev Recd Date: 2016-11-01

Key words:

Abstract: Objective To assay Icariine, Epimedin C, asperosaponin Ⅵ, psoralen and angelicin in Xianlinggubao capsules via multi-wavelength HPLC method. Methods Separation was carried out on Welch Ultimate^® XB-C₁₈ column. The mobile phase was acetonitrile-water system and a linear gradient elution was used. The column temperature was 30 ℃. The detection wavelength for Icariine, Epimedin C,asperosaponin Ⅵ was set at 212 nm, psoralen and angelicin at 246 nm. Results Five components reached baseline separation, the linearity was good when sample size was in the range of 0.008 2-0.328 μg for Icariine(r=0.999 5), 0.055 6-2.224 μg for Epimedin C (r=0.999 6), 0.144 1-5.764 μg for asperosaponin Ⅵ(r=0.999 6), 0.005 4-0.215 2 μg for psoralen(r=0.998 0), 0.006 6-0.265 6 μg for angelicin(r=0.998 5). The average recoveries were 97.59%, 98.58%, 98.11%, 97.86%, 98.22% respectively. The RSDs of recovery were all less than 2.0%. Conclusion This method is simple, accurate, with good separation, high sensitivity for the assay of multiple components in Xianlinggubao capsule.

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Reference (7)

[1]	国家药典委员会.国家食品药品监督管理局国家药品标准［S］.WS-10269(ZD-0269)-2002-2011Z,2011.
[2]	伍庆,周宁,冯泽熹,等.HPLC测定仙灵骨葆胶囊中淫羊藿苷和淫羊藿定C含量［J］.中成药,2009,31(8):1211-1213.
[3]	李振国,李忠保,王海波.HPLC法测定仙灵骨葆颗粒中补骨脂素和异补骨脂素的含量［J］.中医研究,2009,22(1):25-26.
[4]	吴春园,王俊,高锦飚.高效液相色谱法测定仙灵骨葆胶囊中川续断皂苷Ⅵ的含量［J］.中国现代药物应用,2014,8(17):238-239.
[5]	高国英,吴越,倪艳娜.HPLC-MS对仙灵骨葆胶囊含量测定方法的探讨［J］.中国药品标准,2010,11(4):298-301.
[6]	钱南萍,万维香,魏润新.反相高效液相色谱法同时测定仙灵骨葆滴丸中淫羊藿苷和补骨脂素及异补骨脂素的含量［J］.中成药,2011,33(2):269-272.
[7]	谢琴,王菊美,黄礼杰,等.仙灵骨葆胶囊多指标成分的定量研究［J］.中成药,2001,23(6):411-414.

[1]	国家药典委员会.国家食品药品监督管理局国家药品标准［S］.WS-10269(ZD-0269)-2002-2011Z,2011.
[2]	伍庆,周宁,冯泽熹,等.HPLC测定仙灵骨葆胶囊中淫羊藿苷和淫羊藿定C含量［J］.中成药,2009,31(8):1211-1213.
[3]	李振国,李忠保,王海波.HPLC法测定仙灵骨葆颗粒中补骨脂素和异补骨脂素的含量［J］.中医研究,2009,22(1):25-26.
[4]	吴春园,王俊,高锦飚.高效液相色谱法测定仙灵骨葆胶囊中川续断皂苷Ⅵ的含量［J］.中国现代药物应用,2014,8(17):238-239.
[5]	高国英,吴越,倪艳娜.HPLC-MS对仙灵骨葆胶囊含量测定方法的探讨［J］.中国药品标准,2010,11(4):298-301.
[6]	钱南萍,万维香,魏润新.反相高效液相色谱法同时测定仙灵骨葆滴丸中淫羊藿苷和补骨脂素及异补骨脂素的含量［J］.中成药,2011,33(2):269-272.
[7]	谢琴,王菊美,黄礼杰,等.仙灵骨葆胶囊多指标成分的定量研究［J］.中成药,2001,23(6):411-414.

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One step assay for five components in Xianlinggubao capsule by HPLC method

doi: 10.3969/j.issn.1006-0111.2017.05.014

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通讯作者: 陈斌, [email protected]

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