Study on quality standard of Wujin capsules

LI Yang; SUN Sen; WANG Hui; ZHANG Guoqing; ZHANG Hai

doi:10.3969/j.issn.1006-0111.2017.05.012

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Objective To establish the qualitative and quantitative analytic method for quality control of Wujin capsules. Methods Curcuma wenyujin Y. H. Chen et C. Ling and Crataegus pinnatifida Bge.in Wujin capsules were identified by TLC method. The content of tenacissoside H was determined by HPLC.Chromatographic separation was achieved at Waters Xbridge C₁₈column (100 mm×3.0 mm, 3.5 μm) with an evaporative light scattering detector (ELSD). Acetonitrile and H₂O were used as the mobile phase for isocratic elution. Results The qualitative identification by TLC was specific. The well separated clear spots were exhibited on each thin layer plate.Tenacissoside H had a linear equation in the range of 11.60-580.00 μg/ml(r=0.999 0). The average recovery was 101.54%. Conclusion The TLC identification of Curcuma wenyujin Y. H. Chen et C. Ling and Crataegus pinnatifida Bge.was simple and well established. The HPLC-ELSD method was specific,sensitive and reliable.Those methods can be used for the quality control of Wujin capsules.

Study on quality standard of Wujin capsules

Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital Affiliated to Second Military Medical University, Shanghai 200438, China

Received Date: 2016-07-01

Rev Recd Date: 2016-11-16

Key words:

Wujin capsules /
Curcuma wenyujin Y. H. Chen et C. Ling /
Crataegus pinnatifida Bge. /
TLC /
tenacissoside H

Abstract: Objective To establish the qualitative and quantitative analytic method for quality control of Wujin capsules. Methods Curcuma wenyujin Y. H. Chen et C. Ling and Crataegus pinnatifida Bge.in Wujin capsules were identified by TLC method. The content of tenacissoside H was determined by HPLC.Chromatographic separation was achieved at Waters Xbridge C₁₈column (100 mm×3.0 mm, 3.5 μm) with an evaporative light scattering detector (ELSD). Acetonitrile and H₂O were used as the mobile phase for isocratic elution. Results The qualitative identification by TLC was specific. The well separated clear spots were exhibited on each thin layer plate.Tenacissoside H had a linear equation in the range of 11.60-580.00 μg/ml(r=0.999 0). The average recovery was 101.54%. Conclusion The TLC identification of Curcuma wenyujin Y. H. Chen et C. Ling and Crataegus pinnatifida Bge.was simple and well established. The HPLC-ELSD method was specific,sensitive and reliable.Those methods can be used for the quality control of Wujin capsules.

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Study on quality standard of Wujin capsules

doi: 10.3969/j.issn.1006-0111.2017.05.012

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通讯作者: 陈斌, [email protected]

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