Determination of seven components in Pifubingxuedu tablet by HPLC method

SHI Zhihui; XIAO Huimin; HE Yue; LIU Yang; LIU Haiyue; YANG Xu; WANG Siwang

doi:10.3969/j.issn.1006-0111.2017.05.010

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Objective To establish a HPLC method for simultaneous determination of seven constituents in Pifubingxuedu tablet. Methods The determination was performed on Kromasil-C₁₈ column (4.6 mm×250 mm, 5 μm).The mobile phase was acetonitrile-0.5% phosphoric acid aqueous solution with a flow rate of 0.8ml/min. The detector was PDA and detection wavelength was set at 245,325,403 nm, respectively. Results A method has been established for the determination of chlorogenic acid, hydroxy Safflower Pigment A, paeoniflorin, forsythiaside A, ferulic acid, berberine and glycyrrhizin acid in one run by HPLC. Their average contents and RSD in each Pifubingxuedu tablet were 0.299 5 mg/tablet (2.25%);0.700 0 mg/tablet(1.33%);0.429 2 mg/tablet (1.21%);0.039 1 mg/tablet (2.34%);0.014 8 mg/tablet(2.23%);0.209 0 mg/tablet (2.06%);0.272 7 mg/tablet (2.68%), respectively. Conclusion The established method is simple, convenient, accurate and reliable. It is suitable for the quality control of Pifubingxuedu tablet.

Determination of seven components in Pifubingxuedu tablet by HPLC method

1. Shaanxi Jun Bi Sha Pharmaceutical Limited Company, Xianyang 712000, China

2. New Materia Medica Research and Development Center, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China

3. Shaanxi University of Chinese Medicine, Xianyang 712046, China

Received Date: 2016-09-05

Rev Recd Date: 2016-12-27

Key words:

Abstract: Objective To establish a HPLC method for simultaneous determination of seven constituents in Pifubingxuedu tablet. Methods The determination was performed on Kromasil-C₁₈ column (4.6 mm×250 mm, 5 μm).The mobile phase was acetonitrile-0.5% phosphoric acid aqueous solution with a flow rate of 0.8ml/min. The detector was PDA and detection wavelength was set at 245,325,403 nm, respectively. Results A method has been established for the determination of chlorogenic acid, hydroxy Safflower Pigment A, paeoniflorin, forsythiaside A, ferulic acid, berberine and glycyrrhizin acid in one run by HPLC. Their average contents and RSD in each Pifubingxuedu tablet were 0.299 5 mg/tablet (2.25%);0.700 0 mg/tablet(1.33%);0.429 2 mg/tablet (1.21%);0.039 1 mg/tablet (2.34%);0.014 8 mg/tablet(2.23%);0.209 0 mg/tablet (2.06%);0.272 7 mg/tablet (2.68%), respectively. Conclusion The established method is simple, convenient, accurate and reliable. It is suitable for the quality control of Pifubingxuedu tablet.

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Determination of seven components in Pifubingxuedu tablet by HPLC method

doi: 10.3969/j.issn.1006-0111.2017.05.010

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