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Volume 35 Issue 5
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2017  >  35(5): 402-406,426

QIAO Jin, CHEN Min, DOU Zhihua, XU Jiliang, WU Feng, MENG Guoliang. Mechanism of TGF-β1/Smad signaling pathway in rhein protected diabetic rat's kidney[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(5): 402-406,426. doi: 10.3969/j.issn.1006-0111.2017.05.004
Citation: QIAO Jin, CHEN Min, DOU Zhihua, XU Jiliang, WU Feng, MENG Guoliang. Mechanism of TGF-β1/Smad signaling pathway in rhein protected diabetic rat's kidney[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(5): 402-406,426. doi: 10.3969/j.issn.1006-0111.2017.05.004
shu

Mechanism of TGF-β1/Smad signaling pathway in rhein protected diabetic rat's kidney

doi: 10.3969/j.issn.1006-0111.2017.05.004
  • 1.

    Department of Pharmacy, Third Hospital Affiliated to Nantong University, Nantong 226006, China

  • 2.

    Department of Pharmacology, Medical College, Nantong University, Nantong 226001, China

  • Received Date: 2017-05-18
  • Rev Recd Date: 2017-06-26
  • Objective To study the protective effect of Rhein on the kidney of type 2 diabetic rats induced by high fat diet. Methods A rat model of type 2 diabetes was induced by high fat diet combined with low dose streptozotocin 35 mg/kg. The diabetic rats were randomly divided into diabetes group, Low, middle and high rhein dose groups (50,100,150 mg/kg), metformin group (300 mg/kg) and normal control group. Blood glucose and urine micro albumin were measured at 0, 2, 4 and 8 weeks respectively. Serum creatinine, urea nitrogen, total cholesterol and triglyceride were measured at 8 weeks. HE staining was used to observe the pathological changes of renal tissue. Effects of rhein on the expression of transforming growth factor-β1 and Smad3 protein in renal tissue of diabetic rats were detected with Western Blot. Results The blood glucose and urine micro albumin in model group were significantly higher than those in normal control group. Each rhein dose group exhibited reduced blood glucose and urinary micro albumin in diabetic rats. The high rhein dose group showed significant reduction of blood glucose and urine micro albumin in diabetic rats (P<0.05). Compared with model group, rhein reduced the serum Cr, BUN, TC and TG values in each dose group, most significantly in the high rhein dose group (P<0.05). The obvious pathological changes of renal tissue in model group were observed with most improved changes in the high rhein dose group. The expression of TGF-β1 and Smad3 protein in renal tissue of diabetic rats decreased significantly (P<0.05). Conclusion Rhein has preventive effect on diabetic nephropathy. The mechanism may relate to the improvement of renal function, regulation of blood lipid and down regulation of TGF-β1 and Smad3 protein expression in renal tissue.
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    [2] Xu Y,Wang L,He J,et al. Prevalence and control of diabetes in Chinese adults［J］.JAMA,2013,310(9):948-959.
    [3] 陈才铭,张苗苗,胡利明.大黄酸对肥胖糖尿病大鼠肾皮质PPARγ_和TGF-β1表达的影响［J］.中药材,2015,38(4):810-812.
    [4] 吴影懿,段俗言,钱 军,等.大黄酸对糖尿病小鼠肾脏的保护作用［J］.江苏医药,2015,41 (16):1864-1866.
    [5] 陈卫东,常保超,张 燕,等.大黄酸增加2型糖尿病大鼠肾组织SIRT1的表达［J］.细胞与分子免疫学杂志,2015,31(5):615-619.
    [6] Geng CC,Chen GR,Wu QJ,et al.The molecular mechanism of rhein in diabetic nephropathy［J］.Evid Based Complement Alternat Med,2014,20(14):487-497.
    [7] 乔 进,窦志华,吴 锋,等.灵芝多糖联合二甲双胍对2型糖尿病大鼠心肌AGEs及CTGF的影响及其机制［J］.中国药理学通报,2014,30(4):536-541.
    [8] 乔 进,窦志华,吴 锋,等.灵芝多糖联合二甲双胍预防糖尿病大鼠主动脉病变及对VEGF表达的影响［J］.中国药理学通报,2014,30(8):1079-1084.
    [9] 乔 进,窦志华,吴 锋,等.灵芝多糖联合二甲双胍对2型糖尿病大鼠心肌结构及血流动力学的影响［J］.中国药理学通报,2016,32(7):1012-1016.
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    [11] Chen Y,Liu Z Y,Zhou F X,et al. Effects of Shuangdan Oral Liquid on diabetic nephropathy rat models［J］.Northwest Pharm J,2016,31(1):56-61.
    [12] 李梦莹,谢春光,王晓灿.大黄酸治疗糖尿病肾病实验研究进展［J］.湖南中医杂志,2015,31(10):172-173.
    [13] Loeffler I,Hopfer U,Koczan D,et al. Type VIII collagen modulater TGF-β1- induced proliferation of mesangial cells［J］.J Am Soc Nephrol, 2011,22(4):649-663.
    [14] Overstreet JM,Samara Koon R,Meldrum KK,et al. Redox control of p53 in the transcriptional regulation of TGF-betal target genes through SMAD cooperativity ［J］.Cell Signal,2014,26(7):1427-1436.
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Mechanism of TGF-β1/Smad signaling pathway in rhein protected diabetic rat's kidney

doi: 10.3969/j.issn.1006-0111.2017.05.004
  • 1. Department of Pharmacy, Third Hospital Affiliated to Nantong University, Nantong 226006, China
  • 2. Department of Pharmacology, Medical College, Nantong University, Nantong 226001, China
  • Received Date: 2017-05-18
  • Rev Recd Date: 2017-06-26

Abstract: Objective To study the protective effect of Rhein on the kidney of type 2 diabetic rats induced by high fat diet. Methods A rat model of type 2 diabetes was induced by high fat diet combined with low dose streptozotocin 35 mg/kg. The diabetic rats were randomly divided into diabetes group, Low, middle and high rhein dose groups (50,100,150 mg/kg), metformin group (300 mg/kg) and normal control group. Blood glucose and urine micro albumin were measured at 0, 2, 4 and 8 weeks respectively. Serum creatinine, urea nitrogen, total cholesterol and triglyceride were measured at 8 weeks. HE staining was used to observe the pathological changes of renal tissue. Effects of rhein on the expression of transforming growth factor-β1 and Smad3 protein in renal tissue of diabetic rats were detected with Western Blot. Results The blood glucose and urine micro albumin in model group were significantly higher than those in normal control group. Each rhein dose group exhibited reduced blood glucose and urinary micro albumin in diabetic rats. The high rhein dose group showed significant reduction of blood glucose and urine micro albumin in diabetic rats (P<0.05). Compared with model group, rhein reduced the serum Cr, BUN, TC and TG values in each dose group, most significantly in the high rhein dose group (P<0.05). The obvious pathological changes of renal tissue in model group were observed with most improved changes in the high rhein dose group. The expression of TGF-β1 and Smad3 protein in renal tissue of diabetic rats decreased significantly (P<0.05). Conclusion Rhein has preventive effect on diabetic nephropathy. The mechanism may relate to the improvement of renal function, regulation of blood lipid and down regulation of TGF-β1 and Smad3 protein expression in renal tissue.

QIAO Jin, CHEN Min, DOU Zhihua, XU Jiliang, WU Feng, MENG Guoliang. Mechanism of TGF-β1/Smad signaling pathway in rhein protected diabetic rat's kidney[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(5): 402-406,426. doi: 10.3969/j.issn.1006-0111.2017.05.004
Citation: QIAO Jin, CHEN Min, DOU Zhihua, XU Jiliang, WU Feng, MENG Guoliang. Mechanism of TGF-β1/Smad signaling pathway in rhein protected diabetic rat's kidney[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(5): 402-406,426. doi: 10.3969/j.issn.1006-0111.2017.05.004
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