HPLC法同时测定多效防护霜中盐酸苯海拉明、甲氧基肉桂酸辛酯和水杨酸辛酯的含量

韩凌; 王新霞; 吕磊; 张静如; 阚月一; 鲁莹; 傅翔

doi:10.3969/j.issn.1006-0111.2019.06.007

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

HPLC法同时测定多效防护霜中盐酸苯海拉明、甲氧基肉桂酸辛酯和水杨酸辛酯的含量

韩凌, 王新霞, 吕磊, 张静如, 阚月一, 鲁莹, 傅翔

文章导航 > 药学实践与服务 > 2019 > 37(6): 508-511

韩凌, 王新霞, 吕磊, 张静如, 阚月一, 鲁莹, 傅翔. HPLC法同时测定多效防护霜中盐酸苯海拉明、甲氧基肉桂酸辛酯和水杨酸辛酯的含量[J]. 药学实践与服务, 2019, 37(6): 508-511. doi: 10.3969/j.issn.1006-0111.2019.06.007

引用本文:

HAN Ling, WANG Xinxia, LV Lei, ZHANG Jingru, KAN Yueyi, LU Ying, FU Xiang. Simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 508-511. doi: 10.3969/j.issn.1006-0111.2019.06.007

Citation:

HAN Ling, WANG Xinxia, LV Lei, ZHANG Jingru, KAN Yueyi, LU Ying, FU Xiang. Simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 508-511. doi: 10.3969/j.issn.1006-0111.2019.06.007

HPLC法同时测定多效防护霜中盐酸苯海拉明、甲氧基肉桂酸辛酯和水杨酸辛酯的含量

doi: 10.3969/j.issn.1006-0111.2019.06.007

1.
上海控江医院药剂科, 上海 200093;海军军医大学药学院, 上海 200433
2.
上海东方肝胆外科医院药材科, 上海 200438
3.
海军军医大学药学院, 上海 200433
4.
上海控江医院药剂科, 上海 200093

基金项目: 国家科技重大专项资助项目（2018ZX09J18110-001-001）

Simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream by HPLC

1.
Department of Pharmacy, Shanghai Kongjiang Hospital, Shanghai 200093, China;School of Pharmacy, Naval Medical University, Shanghai 200433, China
2.
Department of Pharmacy, Shanghai Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China
3.
School of Pharmacy, Naval Medical University, Shanghai 200433, China
4.
Department of Pharmacy, Shanghai Kongjiang Hospital, Shanghai 200093, China

摘要: 目的建立同时测定多效防护霜中盐酸苯海拉明、甲氧基肉桂酸辛酯和水杨酸辛酯的含量的方法。方法采用高效液相色谱法。色谱柱为Agilent Extend-C₁₈（4.6 mm×150 mm，5 μm），流动相为甲醇-乙酸铵缓冲溶液（20 mmol/L，pH=7.5），梯度洗脱，甲醇比例随时间变化为：0~10 min、甲醇→60%；10~12 min、甲醇60%→92%；12~20 min、甲醇→92%，采集时间20 min，平衡时间10 min，流速1 ml/min，紫外检测波长218 nm，柱温25℃，进样量15 μl。结果 3种成分在30 min内基线分离，方法学验证表明，线性关系良好（r>0.9995），日内日间精密度均小于3.0%，回收率在90%~108%之间，稳定性和重复性RSD均<3.0%，符合方法学要求。按照新建立的方法测定了3个批次样品中3种成分的含量，结果符合要求。结论本方法简便可靠，可为多效防护霜的质量控制提供依据，也为其质量标准研究奠定了基础。
- 多效防护霜 /
- 高效液相色谱 /
- 盐酸苯海拉明 /
- 甲氧基肉桂酸辛酯 /
- 水杨酸辛酯 /
- 含量测定
Abstract: Objective To establish a HPLC method for the simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream. Methods The HPLC condition was as follows.The column was Agilent Extend-C₁₈ (4.6 mm×150 mm,5 μm).The mobile phase was methanol and ammonium acetate buffer solution (20 mmol/L,pH=7.5).Gradient elution.The methanol ratio varies with time as follows:0-10 min,methanol→60%; 10-12 min,methanol 60%→92%;12-20 min,methanol→92%.Acquisition time was 20 minutes.Liquid phase equilibrium time was 10 min.Flow rate was 1 ml/min.UV detection wavelength was 218 nm.Column temperature was 25℃.Injection volume was 15 μl. Results The three components were separated at baseline within 30 min.The methodological verification showed that the standard curves had good linearity (r>0.9995).The inter-day precision were less than 3.0%.The recovery was between 90% and 108%.The RSD% of stability and repeatability were also less than 3.0%.The results showed that the method could meet the requirements of method validation.The contents of the three components in three batches were determined according to the newly established method. Conclusion The method is simple and reliable,can provide a basis for the quality control of multi-effect protective cream,and laid a foundation for its quality standard research.
- multi-effect protective cream /
- HPLC /
- diphenhydramine hydrochloride /
- octyl methoxycinnamate /
- octyl salicylate /
- content determination

[1]	刘鹏,蔡杨.某特种部队海练期间伤病情况分析及对策[J].海军医学杂志,2016,37(4):296-298.
[2]	卢畅,费文超,纪世召,等.海军某士官学校学员基层部队服役期间训练伤发生情况调查[J].解放军预防医学杂志,2017,35(1):16-18.
[3]	Lotan A.Method for inhibiting nematocyst discharge:US,US6406709[P].2002.
[4]	张智.水母蜇伤皮肤防护剂研究[D].上海:第二军医大学,2010.
[5]	Ginestar G J,Panyella C D,Catalá E R,et al.Anti-jellyfish combinations:,EP 2380577 A1[P].2011.
[6]	陈云霞,马强,白桦,等.祛痘化妆品中苯海拉明的高效液相色谱检测及质谱确证[J].分析科学学报,2014,30(4):521-524.
[7]	肖庚鹏,方永培,王水生,等.化妆品中甲氧基肉桂酸辛酯和水杨酸乙基己酯含量的测定[J].日用化学工业,2009,39(1):68-70.
[8]	徐惠金,林峰,吴映璇.高效液相色谱法测定化妆品中9种防晒剂[J].理化检验-化学分册,2015,51(5):621-624.

[1]	景凯, 杨慈荣, 张圳, 臧艺蓓, 刘霞. 黄芪甲苷衍生物治疗慢性心力衰竭小鼠的药效评价及作用机制研究 . 药学实践与服务, 2024, 42(5): 190-197. doi: 10.12206/j.issn.2097-2024.202310004
[2]	张广雨, 杜晶, 刘梦珍, 朱丹妮, 闫慧, 刘冲. 新斯的明与山莨菪碱联合应用对肺型氧中毒的保护作用及其机制的研究 . 药学实践与服务, 2024, 42(10): 433-438, 444. doi: 10.12206/j.issn.2097-2024.202310049
[3]	刘丽艳, 余小翠, 孙传铎. 纳武利尤单抗治疗非小细胞肺癌有效性及安全性的Meta分析 . 药学实践与服务, 2024, 42(10): 451-456. doi: 10.12206/j.issn.2097-2024.202310044
[4]	瞿文君, 白若楠, 崔力, 周琰. 基于联合库存的公立医院多院区药品采购模式分析 . 药学实践与服务, 2024, 42(7): 315-318. doi: 10.12206/j.issn.2097-2024.202401002
[5]	赖立勇, 夏天爽, 徐圣焱, 蒋益萍, 岳小强, 辛海量. 中药青蒿抗氧化活性的谱效关系研究 . 药学实践与服务, 2024, 42(5): 203-210, 216. doi: 10.12206/j.issn.2097-2024.202211012
[6]	舒飞, 孙蕊, 宋凯, 张元林, 闫家铭, 舒丽芯. 粉-液双室袋产品综合评估 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202312009
[7]	毛智毅, 王筱燕, 陈晓颖, 汤逸斐. 度拉糖肽联合二甲双胍对肥胖型2型糖尿病患者机体代谢、体脂成分及血清脂肪因子的影响 . 药学实践与服务, 2024, 42(7): 305-309. doi: 10.12206/j.issn.2097-2024.202305032
[8]	郭灵怡, 刘艳超, 高路, 刘瑞瑶, 吕权真, 俞媛. 醋酸卡泊芬净单硬脂酸甘油酯纳米粒抗白色念珠菌感染的增效作用研究 . 药学实践与服务, 2024, 42(): 1-8. doi: 10.12206/j.issn.2097-2024.202310043
[9]	陈炳辰, 王思真, 郭贝贝, 杨峰. 紫杉醇棕榈酸酯的合成及其脂质体的制备与处方研究 . 药学实践与服务, 2024, 42(9): 379-384, 410. doi: 10.12206/j.issn.2097-2024.202404062
[10]	崔亚玲, 吴琼, 马良煜, 胡北, 姚东, 许子华. 肝素钠肌醇烟酸酯乳膏中肌醇烟酸酯皮肤药动学研究 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202404006
[11]	陈炳辰, 佟达丰, 万苗, 闫飞虎, 姚建忠. UPLC-MS/MS法测定小鼠血浆中紫杉醇脂肪酸酯前药及其药代动力学研究 . 药学实践与服务, 2024, 42(8): 341-345. doi: 10.12206/j.issn.2097-2024.202404082
[12]	陈方剑, 骆锦前, 王志君, 胡叶帅, 孙煜昕, 宋洪杰. HPLC-MS/MS同时测定感冒安颗粒中5种黄酮成分的含量 . 药学实践与服务, 2024, 42(9): 402-406. doi: 10.12206/j.issn.2097-2024.202403030
[13]	姚小静, 计佩影, 陆峰, 施国荣, 傅翔. 表面增强拉曼光谱法快速测定尿液中曲马多的研究 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202401072
[14]	夏哲炜, 曾垣烨, 朱海菲, 李育, 陈啸飞. 核磁共振磷谱法测定磷酸氢钙咀嚼片中药物含量 . 药学实践与服务, 2024, 42(9): 399-401, 406. doi: 10.12206/j.issn.2097-2024.202404063
[15]	李丹, 戴贤春, 王芳珍, 陈奕含, 杨萍, 刘继勇. HPLC-MS/MS测定当归六黄汤中4种不同成分的含量 . 药学实践与服务, 2024, 42(6): 248-252, 266. doi: 10.12206/j.issn.2097-2024.202305007

点击查看大图

计量

文章访问数: 3435
HTML全文浏览量: 446
PDF下载量: 802
被引次数: 0

HPLC法同时测定多效防护霜中盐酸苯海拉明、甲氧基肉桂酸辛酯和水杨酸辛酯的含量

doi: 10.3969/j.issn.1006-0111.2019.06.007

1. 上海控江医院药剂科, 上海 200093;海军军医大学药学院, 上海 200433

2. 上海东方肝胆外科医院药材科, 上海 200438

3. 海军军医大学药学院, 上海 200433

4. 上海控江医院药剂科, 上海 200093

基金项目: 国家科技重大专项资助项目（2018ZX09J18110-001-001）

收稿日期: 2019-08-20
修回日期: 2019-11-04

关键词:

摘要: 目的建立同时测定多效防护霜中盐酸苯海拉明、甲氧基肉桂酸辛酯和水杨酸辛酯的含量的方法。方法采用高效液相色谱法。色谱柱为Agilent Extend-C₁₈（4.6 mm×150 mm，5 μm），流动相为甲醇-乙酸铵缓冲溶液（20 mmol/L，pH=7.5），梯度洗脱，甲醇比例随时间变化为：0~10 min、甲醇→60%；10~12 min、甲醇60%→92%；12~20 min、甲醇→92%，采集时间20 min，平衡时间10 min，流速1 ml/min，紫外检测波长218 nm，柱温25℃，进样量15 μl。结果 3种成分在30 min内基线分离，方法学验证表明，线性关系良好（r>0.9995），日内日间精密度均小于3.0%，回收率在90%~108%之间，稳定性和重复性RSD均<3.0%，符合方法学要求。按照新建立的方法测定了3个批次样品中3种成分的含量，结果符合要求。结论本方法简便可靠，可为多效防护霜的质量控制提供依据，也为其质量标准研究奠定了基础。

English Abstract

Simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream by HPLC

1. Department of Pharmacy, Shanghai Kongjiang Hospital, Shanghai 200093, China;School of Pharmacy, Naval Medical University, Shanghai 200433, China

2. Department of Pharmacy, Shanghai Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China

3. School of Pharmacy, Naval Medical University, Shanghai 200433, China

4. Department of Pharmacy, Shanghai Kongjiang Hospital, Shanghai 200093, China

Received Date: 2019-08-20
Rev Recd Date: 2019-11-04

Keywords:

Abstract: Objective To establish a HPLC method for the simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream. Methods The HPLC condition was as follows.The column was Agilent Extend-C₁₈ (4.6 mm×150 mm,5 μm).The mobile phase was methanol and ammonium acetate buffer solution (20 mmol/L,pH=7.5).Gradient elution.The methanol ratio varies with time as follows:0-10 min,methanol→60%; 10-12 min,methanol 60%→92%;12-20 min,methanol→92%.Acquisition time was 20 minutes.Liquid phase equilibrium time was 10 min.Flow rate was 1 ml/min.UV detection wavelength was 218 nm.Column temperature was 25℃.Injection volume was 15 μl. Results The three components were separated at baseline within 30 min.The methodological verification showed that the standard curves had good linearity (r>0.9995).The inter-day precision were less than 3.0%.The recovery was between 90% and 108%.The RSD% of stability and repeatability were also less than 3.0%.The results showed that the method could meet the requirements of method validation.The contents of the three components in three batches were determined according to the newly established method. Conclusion The method is simple and reliable,can provide a basis for the quality control of multi-effect protective cream,and laid a foundation for its quality standard research.

引用本文:

Citation:

HAN Ling, WANG Xinxia, LV Lei, ZHANG Jingru, KAN Yueyi, LU Ying, FU Xiang. Simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 508-511. doi: 10.3969/j.issn.1006-0111.2019.06.007

全文HTML

参考文献 (8)

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

主管、主办: 中国人民解放军海军军医大学地址: 上海市杨浦区国和路325号邮政编码: 200433

电话: （021）81871324,81871397 E-mail: [email protected]

网站备案号沪ICP备05003363号-1

本系统由北京仁和汇智信息技术有限公司开发

留言板

HPLC法同时测定多效防护霜中盐酸苯海拉明、甲氧基肉桂酸辛酯和水杨酸辛酯的含量

doi: 10.3969/j.issn.1006-0111.2019.06.007

Simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream by HPLC

计量

出版历程

HPLC法同时测定多效防护霜中盐酸苯海拉明、甲氧基肉桂酸辛酯和水杨酸辛酯的含量

doi: 10.3969/j.issn.1006-0111.2019.06.007

1. 上海控江医院药剂科, 上海 200093;海军军医大学药学院, 上海 200433 2. 上海东方肝胆外科医院药材科, 上海 200438 3. 海军军医大学药学院, 上海 200433 4. 上海控江医院药剂科, 上海 200093

English Abstract

Simultaneous determination of diphenhydramine hydrochloride,octyl methoxycinnamate and octyl salicylate in multi-effect protective cream by HPLC

全文HTML

目录

1. 上海控江医院药剂科, 上海 200093;海军军医大学药学院, 上海 200433

2. 上海东方肝胆外科医院药材科, 上海 200438

3. 海军军医大学药学院, 上海 200433

4. 上海控江医院药剂科, 上海 200093