基于正交投影偏最小二乘法对复方首乌藤合剂抗失眠作用的谱效关系研究

曾棋平; 蔡小辉; 吴坤林; 杨丽娜; 曹毅祥; 陈锦珊

doi:10.3969/j.issn.1006-0111.2019.05.008

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基于正交投影偏最小二乘法对复方首乌藤合剂抗失眠作用的谱效关系研究

曾棋平, 蔡小辉, 吴坤林, 杨丽娜, 曹毅祥, 陈锦珊

文章导航 > 药学实践与服务 > 2019 > 37(5): 427-432

曾棋平, 蔡小辉, 吴坤林, 杨丽娜, 曹毅祥, 陈锦珊. 基于正交投影偏最小二乘法对复方首乌藤合剂抗失眠作用的谱效关系研究[J]. 药学实践与服务, 2019, 37(5): 427-432. doi: 10.3969/j.issn.1006-0111.2019.05.008

引用本文:

ZENG Qiping, CAI Xiaohui, WU Kunlin, YANG Lina, CAO Yixiang, CHEN Jinshan. Study on spectrum-effect relationship of sedative and hypnotic effect of compound caulis polygoni multiflori mixture based on orthogonal partial least squares regression analysis[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 427-432. doi: 10.3969/j.issn.1006-0111.2019.05.008

Citation:

ZENG Qiping, CAI Xiaohui, WU Kunlin, YANG Lina, CAO Yixiang, CHEN Jinshan. Study on spectrum-effect relationship of sedative and hypnotic effect of compound caulis polygoni multiflori mixture based on orthogonal partial least squares regression analysis[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 427-432. doi: 10.3969/j.issn.1006-0111.2019.05.008

基于正交投影偏最小二乘法对复方首乌藤合剂抗失眠作用的谱效关系研究

doi: 10.3969/j.issn.1006-0111.2019.05.008

中国人民解放军联勤保障部队第九○九医院/厦门大学附属东南医院制剂科, 福建漳州, 363000

基金项目: 医院青年苗圃课题（16Y024）；福建中医药大学临床专项校管课题（XB2017080）

Study on spectrum-effect relationship of sedative and hypnotic effect of compound caulis polygoni multiflori mixture based on orthogonal partial least squares regression analysis

Department of Pharmacy, No. 909 Hospital of Joint Logistic Support Force/Southeast Hospital Affiliated to Xiamen University, Zhangzhou 363000, China

摘要: 目的探讨复方首乌藤合剂抗失眠作用与HPLC指纹图谱间的相关性。方法采用HPLC分析得到不同提取工艺条件下的复方首乌藤合剂指纹图谱；腹腔注射DL-4-氯苯丙氨酸建立大鼠失眠模型，用ELISA法测定大鼠海马体5-HT的含量，采用正交投影偏最小二乘法分析谱效关系。结果从指纹图谱中共确定21个共有峰，12个峰的变量投影重要性（VIP）均>1.0，复方首乌藤合剂指纹图谱中3个色谱峰对抗失眠发挥重要作用。结论复方首乌藤合剂抗失眠作用的物质基础与其所含多种成分共同作用的结果有关，通过谱效关系分析得到复方首乌藤合剂的抗失眠物质基础，可为制剂的质量控制提供实验依据。
- 复方首乌藤合剂 /
- 指纹图谱 /
- 抗失眠 /
- 谱效关系 /
- 正交投影偏最小二乘法
Abstract: Objective To investigate the correlation between anti-insomnia effects and HPLC fingerprint of compound caulis polygoni multiflori mixture. Methods A fingerprinting method was established by high performance liquid chromatography and then used to analyze different batches of compound caulis polygoni multiflori mixture.Rat insomnia model was established by intraperitoneal injection of p-chlorophenylalanine,5-HT in rat hippocampus was determined by ELISA.Then the orthogonal partial least squares (OPLS)analysis was used to investigate the spectrum-effect relationship. Results 21 common peaks were identified from the fingerprints,the variable projection importance (VIP)values of these 12 peaks were more than 1.0,indicated that the 3 peaks had played an important role in the anti-insomnia activity of compound caulis polygoni multiflori mixture. Conclusion The anti-insomnia activity of compound caulis polygoni multiflori mixture was the result of synergistic effect of multiple components,its material basis could be analyzed by spectrum-effect relationship,which could provide basis for the quality control of preparation.
- compound caulis polygoni multiflori mixture /
- fingerprint /
- anti-insomnia /
- spectrum-effect relationship /
- orthogonal partial least squares

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基于正交投影偏最小二乘法对复方首乌藤合剂抗失眠作用的谱效关系研究

doi: 10.3969/j.issn.1006-0111.2019.05.008

中国人民解放军联勤保障部队第九○九医院/厦门大学附属东南医院制剂科, 福建漳州, 363000

基金项目: 医院青年苗圃课题（16Y024）；福建中医药大学临床专项校管课题（XB2017080）

收稿日期: 2019-03-15
修回日期: 2019-05-22

关键词:

抗失眠 /

摘要: 目的探讨复方首乌藤合剂抗失眠作用与HPLC指纹图谱间的相关性。方法采用HPLC分析得到不同提取工艺条件下的复方首乌藤合剂指纹图谱；腹腔注射DL-4-氯苯丙氨酸建立大鼠失眠模型，用ELISA法测定大鼠海马体5-HT的含量，采用正交投影偏最小二乘法分析谱效关系。结果从指纹图谱中共确定21个共有峰，12个峰的变量投影重要性（VIP）均>1.0，复方首乌藤合剂指纹图谱中3个色谱峰对抗失眠发挥重要作用。结论复方首乌藤合剂抗失眠作用的物质基础与其所含多种成分共同作用的结果有关，通过谱效关系分析得到复方首乌藤合剂的抗失眠物质基础，可为制剂的质量控制提供实验依据。

English Abstract

Study on spectrum-effect relationship of sedative and hypnotic effect of compound caulis polygoni multiflori mixture based on orthogonal partial least squares regression analysis

Department of Pharmacy, No. 909 Hospital of Joint Logistic Support Force/Southeast Hospital Affiliated to Xiamen University, Zhangzhou 363000, China

Received Date: 2019-03-15
Rev Recd Date: 2019-05-22

Keywords:

compound caulis polygoni multiflori mixture /
fingerprint /
anti-insomnia /
spectrum-effect relationship /
orthogonal partial least squares

Abstract: Objective To investigate the correlation between anti-insomnia effects and HPLC fingerprint of compound caulis polygoni multiflori mixture. Methods A fingerprinting method was established by high performance liquid chromatography and then used to analyze different batches of compound caulis polygoni multiflori mixture.Rat insomnia model was established by intraperitoneal injection of p-chlorophenylalanine,5-HT in rat hippocampus was determined by ELISA.Then the orthogonal partial least squares (OPLS)analysis was used to investigate the spectrum-effect relationship. Results 21 common peaks were identified from the fingerprints,the variable projection importance (VIP)values of these 12 peaks were more than 1.0,indicated that the 3 peaks had played an important role in the anti-insomnia activity of compound caulis polygoni multiflori mixture. Conclusion The anti-insomnia activity of compound caulis polygoni multiflori mixture was the result of synergistic effect of multiple components,its material basis could be analyzed by spectrum-effect relationship,which could provide basis for the quality control of preparation.

引用本文:

Citation:

ZENG Qiping, CAI Xiaohui, WU Kunlin, YANG Lina, CAO Yixiang, CHEN Jinshan. Study on spectrum-effect relationship of sedative and hypnotic effect of compound caulis polygoni multiflori mixture based on orthogonal partial least squares regression analysis[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 427-432. doi: 10.3969/j.issn.1006-0111.2019.05.008