来曲唑联合45%高脂饲料诱导生殖内分泌及代谢表型异常的多囊卵巢综合征大鼠模型研究

尹萍; 路璐; 陈媛媛; 李丽; 李赟; 童国庆

doi:10.3969/j.issn.1006-0111.2019.05.007

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来曲唑联合45%高脂饲料诱导生殖内分泌及代谢表型异常的多囊卵巢综合征大鼠模型研究

尹萍, 路璐, 陈媛媛, 李丽, 李赟, 童国庆

文章导航 > 药学实践与服务 > 2019 > 37(5): 422-426

尹萍, 路璐, 陈媛媛, 李丽, 李赟, 童国庆. 来曲唑联合45%高脂饲料诱导生殖内分泌及代谢表型异常的多囊卵巢综合征大鼠模型研究[J]. 药学实践与服务, 2019, 37(5): 422-426. doi: 10.3969/j.issn.1006-0111.2019.05.007

引用本文:

YIN Ping, LU Lu, CHEN Yuanyuan, LI Li, LI Yun, TONG Guoqing. Effects of letrozole and 45% high-fat diet on induction of rat model of polycystic ovary syndrome with reproductive and metabolic disorder[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 422-426. doi: 10.3969/j.issn.1006-0111.2019.05.007

Citation:

YIN Ping, LU Lu, CHEN Yuanyuan, LI Li, LI Yun, TONG Guoqing. Effects of letrozole and 45% high-fat diet on induction of rat model of polycystic ovary syndrome with reproductive and metabolic disorder[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 422-426. doi: 10.3969/j.issn.1006-0111.2019.05.007

来曲唑联合45%高脂饲料诱导生殖内分泌及代谢表型异常的多囊卵巢综合征大鼠模型研究

doi: 10.3969/j.issn.1006-0111.2019.05.007

上海中医药大学附属曙光医院, 上海 200120

基金项目: 上海中医药大学2016年预算内项目（2016N47）

Effects of letrozole and 45% high-fat diet on induction of rat model of polycystic ovary syndrome with reproductive and metabolic disorder

Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200120, China

摘要: 目的建立来曲唑联合高脂饲料诱导SD雌性大鼠多囊卵巢综合征（PCOS）内分泌及代谢异常的动物模型。方法选取18只21日龄雌性大鼠喂养45%高脂饲料16周，第6周龄开始服用来曲唑直至造模结束；8只对照组大鼠常规饲料喂养，第6周龄服用生理盐水。观察阴道涂片及卵巢组织学变化，比较每周体重变化，测定脏器重量、血清睾酮水平、糖耐量、血脂水平、空腹胰岛素以及胰岛素敏感指数。结果与对照组比较，模型组大鼠体重增加，卵巢、腹股沟脂肪质量增加，子宫、垂体质量及脏器指数显著减少。模型组大鼠卵巢呈多囊样改变，颗粒细胞层减少，白膜增加。模型组大鼠的三酰甘油和低密度脂蛋白升高，糖耐量曲线下面积增加，血清睾酮水平上升，空腹胰岛素水平升高，胰岛素敏感性降低。结论来曲唑联合高脂饲料可以建立有效的内分泌及代谢表型异常PCOS动物模型。
- 来曲唑 /
- 高脂饲料 /
- 多囊卵巢综合征 /
- 内分泌 /
- 糖脂代谢 /
- 动物模型
Abstract: Objective To establish rat polycystic ovary syndrome model with letrozole combined high fat diet. Methods Eighteen 21-day-old female rats were fed with 45% high-fat diet for 16 weeks.Letrozole was started at the 6th week of age until the end of modeling.Eight rats in control group were fed with regular diet and Saline without letrozole at the 6th week of age.The vaginal smear and ovarian histological changes were investigated.The changes in body mass,organ weight,blood testosterone level,glucose tolerance,blood lipid level,fasting insulin and insulin sensitivity index were measured. Results In the model group,the ovary of the rats showed a multi-cystic change,the granulosa cell layer decreased,and the tunica albuginea increased.Compared with the control group,the body weight of the model group increased,the weight of the ovary and inguinal fat increased,the weight and weight index of the uterus and pituitary gland decreased,the triglyceride and low density lipoprotein increased,the area under the glucose tolerance curve increased,the blood testosterone level increased,the fasting insulin increased,and the insulin sensitivity decreased. Conclusion Letrozole combined with high fat diet could establish an effective PCOS model of abnormal endocrine and metabolic phenotype.
- letrozole /
- high-fat diets /
- polycystic ovary syndrome /
- reproductive endocrine /
- glucose and lipid metabolism /
- animal model

[1]	ROSENFIELD R L,EHRMANN D A.The pathogenesis of polycystic ovary syndrome(PCOS):the hypothesis of PCOS as functional ovarian hyperandrogenism revisited[J].Endocr Rev,2016,37(5):467-520.
[2]	林莺,张宏,黄鸣清,等.来曲唑配合高脂膳食诱导胰岛素抵抗多囊卵巢综合征大鼠代谢表型的实验研究[J].中国现代医学杂志,2016,26(3):9-13.
[3]	HART R,DOHERTY D A.The potential implications of a PCOS diagnosis on a woman's long-term health using data linkage[J].J Clin Endocrinol Metab,2015,100(3):911-919.
[4]	MORAN L J,MISSO M L,WILD R A,et al.Impaired glucose tolerance,type 2 diabetes and metabolic syndrome in polycystic ovary syndrome:a systematic review and meta-analysis[J].Human Reprod Update,2010,16(4):347-363.
[5]	李明明,潘文,康开彪,等.不同造模方法对大鼠多囊卵巢综合征伴胰岛素抵抗模型的影响[J].西部中医药,2015,28(4):11-14.
[6]	PADMANABHAN V,VEIGA-LOPEZ A.Animal models of the polycystic ovary syndrome phenotype[J].Steroids,2013,78(8):734-740.
[7]	杜丹峰,李雪莲.多囊卵巢综合征动物模型的胰岛素抵抗表现[J].国际妇产科学杂志,2013,40(1):24-26,39.
[8]	PAIXAO L,RAMOS R B,LAVARDA A,et al.Animal models of hyperandrogenism and ovarian morphology changes as features of polycystic ovary syndrome:a systematic review[J].Reprod Biol Endocrinol,2017,15(1):12.
[9]	PAIXAO L,RAMOS R B,LAVARDA A,et al.Animal models of hyperandrogenism and ovarian morphology changes as features of polycystic ovary syndrome:a systematic review[J].Reprod Biol Endocrinol,2017,15:12.
[10]	MANNERAS L,CAJANDER S,HOLMANG A,et al.A new rat model exhibiting both ovarian and metabolic characteristics of polycystic ovary syndrome[J].Endocrinology,2007,148(8):3781-3791.
[11]	GOZUKARA I,DOKUYUCU R,ÖZGVR T,et al.Histopathologic and metabolic effect of ursodeoxycholic acid treatment on PCOS rat model[J].Gynecolog Endocrinol,2016,32(6):492-497.
[12]	MALIQUEO M,SUN M,JOHANSSON J,et al.Continuous administration of a P450 aromatase inhibitor induces polycystic ovary syndrome with a metabolic and endocrine phenotype in female rats at adult age[J].Endocrinology,2013,154(1):434-445.
[13]	SKARRA D V,HERNANDEZ-CARRETERO A,RIVERA A J,et al.Hyperandrogenemia induced by letrozole treatment of pubertal female mice results in hyperinsulinemia prior to weight gain and insulin resistance[J].Endocrinology,2017,158(9):2988-3003.
[14]	韩静,余俊达,潘秋,等.四种高脂饲料诱发胰岛素抵抗的实验研究[J].江西中医学院学报,2010,22(4):64-66.
[15]	张涛,刘源,赵爽,等.不同高能饮食诱发胰岛素抵抗大鼠模型的比较[J].实验动物科学,2011,28(3):29-33.
[16]	RESSLER I B,GRAYSON B E,ULRICH-LAI Y M,et al.Diet-induced obesity exacerbates metabolic and behavioral effects of polycystic ovary syndrome in a rodent model[J].Am J Physiol-Endocrin Metab,2015,308(12):E1076-E1084.
[17]	朱磊,路瑛丽,冯连世,等.不同配方高脂饲料构建SD大鼠肥胖模型的实验研究[J].中国运动医学杂志,2016,35(7):642-647.
[18]	BALASUBRAMANIAN P,JAGANNATHAN L,MAHALEY R E,et al.High fat diet affects reproductive functions in female diet-induced obese and dietary resistant rats[J].J Neuroendocrinol,2012,24(5):748-755.
[19]	REZVANFAR M A,SHOJAEI SAADI H A,GOOSHE M,et al.Ovarian aging-like phenotype in the hyperandrogenism-induced murine model of polycystic ovary[J].Oxid Med Cell Longev,2014,2014:1-10.

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来曲唑联合45%高脂饲料诱导生殖内分泌及代谢表型异常的多囊卵巢综合征大鼠模型研究

doi: 10.3969/j.issn.1006-0111.2019.05.007

上海中医药大学附属曙光医院, 上海 200120

基金项目: 上海中医药大学2016年预算内项目（2016N47）

收稿日期: 2018-12-15
修回日期: 2019-04-22

关键词:

来曲唑 /

内分泌 /

摘要: 目的建立来曲唑联合高脂饲料诱导SD雌性大鼠多囊卵巢综合征（PCOS）内分泌及代谢异常的动物模型。方法选取18只21日龄雌性大鼠喂养45%高脂饲料16周，第6周龄开始服用来曲唑直至造模结束；8只对照组大鼠常规饲料喂养，第6周龄服用生理盐水。观察阴道涂片及卵巢组织学变化，比较每周体重变化，测定脏器重量、血清睾酮水平、糖耐量、血脂水平、空腹胰岛素以及胰岛素敏感指数。结果与对照组比较，模型组大鼠体重增加，卵巢、腹股沟脂肪质量增加，子宫、垂体质量及脏器指数显著减少。模型组大鼠卵巢呈多囊样改变，颗粒细胞层减少，白膜增加。模型组大鼠的三酰甘油和低密度脂蛋白升高，糖耐量曲线下面积增加，血清睾酮水平上升，空腹胰岛素水平升高，胰岛素敏感性降低。结论来曲唑联合高脂饲料可以建立有效的内分泌及代谢表型异常PCOS动物模型。

English Abstract

Effects of letrozole and 45% high-fat diet on induction of rat model of polycystic ovary syndrome with reproductive and metabolic disorder

Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200120, China

Received Date: 2018-12-15
Rev Recd Date: 2019-04-22

Keywords:

Abstract: Objective To establish rat polycystic ovary syndrome model with letrozole combined high fat diet. Methods Eighteen 21-day-old female rats were fed with 45% high-fat diet for 16 weeks.Letrozole was started at the 6th week of age until the end of modeling.Eight rats in control group were fed with regular diet and Saline without letrozole at the 6th week of age.The vaginal smear and ovarian histological changes were investigated.The changes in body mass,organ weight,blood testosterone level,glucose tolerance,blood lipid level,fasting insulin and insulin sensitivity index were measured. Results In the model group,the ovary of the rats showed a multi-cystic change,the granulosa cell layer decreased,and the tunica albuginea increased.Compared with the control group,the body weight of the model group increased,the weight of the ovary and inguinal fat increased,the weight and weight index of the uterus and pituitary gland decreased,the triglyceride and low density lipoprotein increased,the area under the glucose tolerance curve increased,the blood testosterone level increased,the fasting insulin increased,and the insulin sensitivity decreased. Conclusion Letrozole combined with high fat diet could establish an effective PCOS model of abnormal endocrine and metabolic phenotype.

引用本文:

Citation:

YIN Ping, LU Lu, CHEN Yuanyuan, LI Li, LI Yun, TONG Guoqing. Effects of letrozole and 45% high-fat diet on induction of rat model of polycystic ovary syndrome with reproductive and metabolic disorder[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(5): 422-426. doi: 10.3969/j.issn.1006-0111.2019.05.007