Study on extraction process of Ligustrum lucidum Ait
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摘要: 目的 优化女贞子的最佳提取条件。 方法 采用正交试验法设计实验,考察乙醇浓度、加醇量、提取时间和提取次数,通过齐墩果酸、特女贞苷含量测定及浸膏得率来确定最佳乙醇提取工艺;考察粉碎度对女贞子提取工艺的影响,比较女贞子提取液趁热过滤浓缩与静置24 h后过滤浓缩的结果差异,并比较女贞子醇提工艺和水提工艺的各项指标。 结果 女贞子最佳提取工艺为加10倍量的70%乙醇,提取3次,每次1 h,提取液趁热过滤浓缩。 结论 该优化提取工艺合理可行,更适合生产。Abstract: Objective To optimized the ethanol extraction process of Ligustrum lucidum Ait. Methods L9(34) orthogonal experiment was adopted with the change of the contents of oleanolic acid,specnuezhenide and extraction yield.The best ethanol extraction process was optimized by concentration of ethanol,the amount of ethanol,extraction time and extraction times.The effects of the extraction process of Ligustrum lucidum Ait such as crushing degrees and different solvents were inspected in this experiment-.The extraction process was selected from the extraction which was filtered hot and concentrated or filtered and concentrated after standing for 24 h. Results The best extraction process of Ligustrum lucidum Ait was extracted in 10 times 70% ethanol solution for 3 times and heated for 1 hour per time,and then the extraction was filtered hot and concentrated. Conclusion The extraction process of Ligustrum lucidum Ait was reasonable which was more suitable for production.
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Key words:
- Ligustrum lucidum Ait /
- orthogonal test /
- oleanolic acid /
- specnuezhenide
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[1] 国家药典委员会.中华人民共和国药典(一部):2015年版[S].北京:中国医药科技出版社,2015. [2] 刘亭亭,王萌.女贞子化学成分与药理作用研究进展[J].中国实验方剂学杂志,2014,20(14):228-234. [3] 李建芬.中药女贞子研究进展[J].内蒙古中医药,2012,31(16):45-46. [4] 郭姣,何伟,陈宝田,等.复方参术调脂胶囊提取工艺的研究[J].中药材,2006,29(6):598-601. [5] 王奇,芦柏震.齐墩果酸的研究进展[J].中国药房,2008,19(9):711-712. [6] 何新蕾,于栋.响应面优化女贞子齐墩果酸提取工艺研究[J].食品工业,2014,35(7):92-95. [7] 常星洁,刘汉清,邹建荣,等.响应面法优化女贞子有效成分的提取工艺[J].中成药,2012,34(5):839-842. [8] 郭宇洁,任烨,葛争艳,等.正交试验法比较女贞子中齐墩果酸与特女贞苷的乙醇提取工艺[J].中成药,2013,35(2):277-281. [9] 张艳,陈健,陈海华,等.正交试验法优选女贞子中特女贞苷的提取工艺[J].中国兽药杂志,2012,46(10):33-35. [10] 杨丽萍,陈继舜,李志浩.HPLC法测定女贞二至膏中特女贞苷的含量[J].儿科药学杂志,2012,18(7):40-42. [11] 赵蓉.正交试验法优选女贞子中齐墩果酸的提取工艺[J].海峡药学,2007,19(12):18-20. [12] 彭婷婷,王卫华.高效液相色谱法测定女贞子中齐墩果酸的含量[J].医学理论与实践,2011,24(20):2503-2505. [13] 任爱农,卢爱玲,田耀洲,等.层次分析法用于中药复方提取工艺的多指标权重研究[J].中国中药杂志,2008,33(4):372-374.
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