摘要:
目的 建立双波长同时测定复方首乌藤合剂中盐酸川芎嗪、绿原酸、阿魏酸、二苯乙烯苷、合欢皮木脂素苷、川续断皂苷Ⅵ、五味子醇甲的方法。 方法 采用高效液相色谱法，Agilent ZORBAX SB-C18 色谱柱（4.6 mm×250 mm， 5 μm），以乙腈-0.1%磷酸溶液梯度洗脱，流速：1.0 ml/min，检测波长：210 nm（盐酸川芎嗪、合欢皮木脂素苷、川续断皂苷Ⅵ、五味子醇甲）、320 nm（绿原酸、阿魏酸、二苯乙烯苷），柱温：25℃。 结果 盐酸川芎嗪、绿原酸、阿魏酸、二苯乙烯苷、合欢皮木脂素苷、川续断皂苷Ⅵ、五味子醇甲的线性关系良好（r ≥ 0.999 8），平均加样回收率分别为102.33%、100.29%、100.18%、99.95%、99.23%、101.61%、99.28%，RSD 均小于3%。 结论 该法准确可靠，结果稳定，重复性好，可用于复方首乌藤合剂的质量控制。
Abstract:
Objective To establish a double wavelength method for the simultaneous determination of ligustrazine hydrochloride, chlorogenic acid, ferulic acid, stibene glucoside, Lignin glycosides, asperosaponin Ⅵ and schizandrol A in compound caulis polygoni multiflori mixture. Methods The HPLC separation was performed on an Agilent ZORBAX SB-C18 column (250 mm×4.6 mm, 5 μm), gradient eluted with the mixture of acetonitrile and 0.1% phosphoric acid as mobile phase, the flow rate was 1 ml/min, the detection wavelength was 210 nm and 320 nm, and the column temperature was 25℃. Results There were good linear relationships in the determination of ligustrazine hydrochloride, chlorogenic acid, ferulic acid, stibene glucoside, Lignin glycosides, asperosaponin Ⅵ and schizandrol A (r ≥ 0.9997), with the average recovery rate of 102.33%, 100.29%, 100.18%, 99.95%, 99.23%, 101.61%, 99.28%, and the RSD were less than 3%. Conclusion The method was accurate, stable and reproducible and could be used for quality control of compound caulis polygoni multiflori mixture.
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