基于UV法和HPLC法考察盐制对泽泻主要药效成分含量的影响

何茂桃; 宋丽; 文霞; 刘兰

doi:10.3969/j.issn.1006-0111.2018.03.008

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基于UV法和HPLC法考察盐制对泽泻主要药效成分含量的影响

何茂桃, 宋丽, 文霞, 刘兰

文章导航 > 药学实践与服务 > 2018 > 36(3): 228-233

何茂桃, 宋丽, 文霞, 刘兰. 基于UV法和HPLC法考察盐制对泽泻主要药效成分含量的影响[J]. 药学实践与服务, 2018, 36(3): 228-233. doi: 10.3969/j.issn.1006-0111.2018.03.008

引用本文:

HE Maotao, SONG Li, WEN Xia, LIU Lan. The effect of salted processing on the contents of main effective constituents in Alisma Rhizoma by UV and HPLC[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 228-233. doi: 10.3969/j.issn.1006-0111.2018.03.008

Citation:

HE Maotao, SONG Li, WEN Xia, LIU Lan. The effect of salted processing on the contents of main effective constituents in Alisma Rhizoma by UV and HPLC[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 228-233. doi: 10.3969/j.issn.1006-0111.2018.03.008

基于UV法和HPLC法考察盐制对泽泻主要药效成分含量的影响

doi: 10.3969/j.issn.1006-0111.2018.03.008

成都医学院药学院, 四川成都 610500

基金项目: 成都医学院大学生创新实验计划项目（CXJS201415）；四川省教育厅科研项目（14ZB0239）；四川省大学生创新创业训练计划项目（201413705037）

The effect of salted processing on the contents of main effective constituents in Alisma Rhizoma by UV and HPLC

School of Pharmacy, Chengdu College of Medicine, Chengdu 610500, China

摘要: 目的考察盐制对泽泻中总泽泻醇、24-乙酰泽泻醇A和23-乙酰泽泻醇B含量的影响。方法采用紫外分光光度计检测（UV）法测定生泽泻和盐泽泻中总泽泻醇的含量，显色剂为间硝基苯和氢氧化钾，检测波长545 nm；采用HPLC法测定生泽泻和盐泽泻中24-乙酰泽泻醇A和23-乙酰泽泻醇B的含量，色谱柱为依利特C₁₈柱（4.6 mm×250 mm，5 μm），流动相为乙腈-水梯度洗脱，检测波长208 nm，柱温25℃，流速1 ml/min。结果 UV法和HPLC法中，总泽泻醇、24-乙酰泽泻醇A和23-乙酰泽泻醇B在考察的线性范围内，线性关系良好（r>0.999 0）；在生泽泻和盐泽泻中的平均回收率为97.81%~100.05%，RSD<3%。三者在10批生泽泻样品中的平均含量分别为4.41%、0.208%、0.065%；在10批盐泽泻样品中的平均含量分别为9.62%、0.232%、0.017%。结论盐制对泽泻中总泽泻醇、24-乙酰泽泻醇A和23-乙酰泽泻醇B含量影响较大，其中总泽泻醇的含量明显增加，24-乙酰泽泻醇A的含量略有增加，而23-乙酰泽泻醇B的含量明显降低。
- 泽泻 /
- 三萜类成分 /
- 盐制 /
- 高效液相色谱 /
- 紫外分光光度计检测
Abstract: Objective To observe the effect of salted processing on the contents of total alismol, 24-acetylalisol A and 23-acetylalisol B in Alisma Rhizoma. Methods UV was used for the determination of total alismol's content in crude Alisma Rhizoma and salted Alisma Rhizoma with Meta Nitrobenzene and Potassium Hydroxide as the developers. The detection wavelength was 545 nm. HPLC was used for the determination of 24-acetylalisol A's and 23-acetylalisol B's contents in crude Alisma Rhizoma and salted Alisma Rhizoma with Elite C₁₈ (4.6 mm×250 mm, 5 μm) as the chromatographic column. The mobile phase was gradient elution with acetonitrile and water. The detection wavelength was 208 nm. The column temperature was set at 25℃. The flow rate was 1 ml/min. Results The linear relationships of total alismol, 24-acetylalisol A and 23-acetylalisol B were fine (r>0.999 0) in the linear ranges by UV and HPLC. The average recoveries of them in crude Alisma Rhizoma and salted Alisma Rhizoma ranged from 97.81%-100.05%,with the RSD less than 3%. The average contents of total alismol, 24-acetylalisol A and 23-acetylalisol B in ten batches of crude Alisma Rhizoma were 4.41%, 0.208%, 0.065% respectively. The average contents of them in ten batches of salted Alisma Rhizoma were 9.62%, 0.232%, 0.017% respectively. Conclusion The contents of total alismol, 24-acetylalisol A and 23-acetylalisol B in Alisma Rhizoma were influenced considerably by salted processing. The content of total alismol increased obviously. The content of 24-acetylalisol A increased slightly. The content of 23-acetylalisol B decreased obviously.
- Alisma Rhizoma /
- triterpenoids /
- salted processing /
- HPLC /
- UV

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基于UV法和HPLC法考察盐制对泽泻主要药效成分含量的影响

doi: 10.3969/j.issn.1006-0111.2018.03.008

成都医学院药学院, 四川成都 610500

基金项目: 成都医学院大学生创新实验计划项目（CXJS201415）；四川省教育厅科研项目（14ZB0239）；四川省大学生创新创业训练计划项目（201413705037）

收稿日期: 2017-07-11
修回日期: 2018-01-20

关键词:

泽泻 /

三萜类成分 /

盐制 /

高效液相色谱 /

紫外分光光度计检测

摘要: 目的考察盐制对泽泻中总泽泻醇、24-乙酰泽泻醇A和23-乙酰泽泻醇B含量的影响。方法采用紫外分光光度计检测（UV）法测定生泽泻和盐泽泻中总泽泻醇的含量，显色剂为间硝基苯和氢氧化钾，检测波长545 nm；采用HPLC法测定生泽泻和盐泽泻中24-乙酰泽泻醇A和23-乙酰泽泻醇B的含量，色谱柱为依利特C₁₈柱（4.6 mm×250 mm，5 μm），流动相为乙腈-水梯度洗脱，检测波长208 nm，柱温25℃，流速1 ml/min。结果 UV法和HPLC法中，总泽泻醇、24-乙酰泽泻醇A和23-乙酰泽泻醇B在考察的线性范围内，线性关系良好（r>0.999 0）；在生泽泻和盐泽泻中的平均回收率为97.81%~100.05%，RSD<3%。三者在10批生泽泻样品中的平均含量分别为4.41%、0.208%、0.065%；在10批盐泽泻样品中的平均含量分别为9.62%、0.232%、0.017%。结论盐制对泽泻中总泽泻醇、24-乙酰泽泻醇A和23-乙酰泽泻醇B含量影响较大，其中总泽泻醇的含量明显增加，24-乙酰泽泻醇A的含量略有增加，而23-乙酰泽泻醇B的含量明显降低。

English Abstract

The effect of salted processing on the contents of main effective constituents in Alisma Rhizoma by UV and HPLC

School of Pharmacy, Chengdu College of Medicine, Chengdu 610500, China

Received Date: 2017-07-11
Rev Recd Date: 2018-01-20

Keywords:

Abstract: Objective To observe the effect of salted processing on the contents of total alismol, 24-acetylalisol A and 23-acetylalisol B in Alisma Rhizoma. Methods UV was used for the determination of total alismol's content in crude Alisma Rhizoma and salted Alisma Rhizoma with Meta Nitrobenzene and Potassium Hydroxide as the developers. The detection wavelength was 545 nm. HPLC was used for the determination of 24-acetylalisol A's and 23-acetylalisol B's contents in crude Alisma Rhizoma and salted Alisma Rhizoma with Elite C₁₈ (4.6 mm×250 mm, 5 μm) as the chromatographic column. The mobile phase was gradient elution with acetonitrile and water. The detection wavelength was 208 nm. The column temperature was set at 25℃. The flow rate was 1 ml/min. Results The linear relationships of total alismol, 24-acetylalisol A and 23-acetylalisol B were fine (r>0.999 0) in the linear ranges by UV and HPLC. The average recoveries of them in crude Alisma Rhizoma and salted Alisma Rhizoma ranged from 97.81%-100.05%,with the RSD less than 3%. The average contents of total alismol, 24-acetylalisol A and 23-acetylalisol B in ten batches of crude Alisma Rhizoma were 4.41%, 0.208%, 0.065% respectively. The average contents of them in ten batches of salted Alisma Rhizoma were 9.62%, 0.232%, 0.017% respectively. Conclusion The contents of total alismol, 24-acetylalisol A and 23-acetylalisol B in Alisma Rhizoma were influenced considerably by salted processing. The content of total alismol increased obviously. The content of 24-acetylalisol A increased slightly. The content of 23-acetylalisol B decreased obviously.