胰腺癌细胞中TRPV1受体参与EGFR介导的细胞功能研究

黄瑾; 刘景雪; 曾颖; 袁芳

doi:10.3969/j.issn.1006-0111.2018.02.007

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胰腺癌细胞中TRPV1受体参与EGFR介导的细胞功能研究

黄瑾, 刘景雪, 曾颖, 袁芳

文章导航 > 药学实践与服务 > 2018 > 36(2): 126-130

黄瑾, 刘景雪, 曾颖, 袁芳. 胰腺癌细胞中TRPV1受体参与EGFR介导的细胞功能研究[J]. 药学实践与服务, 2018, 36(2): 126-130. doi: 10.3969/j.issn.1006-0111.2018.02.007

引用本文:

黄瑾, 刘景雪, 曾颖, 袁芳. 胰腺癌细胞中TRPV1受体参与EGFR介导的细胞功能研究[J]. 药学实践与服务, 2018, 36(2): 126-130. doi: 10.3969/j.issn.1006-0111.2018.02.007

HUANG Jin, LIU Jingxue, ZENG Ying, YUAN Fang. Transient receptor potential type vanilloid 1 regulates EGFR related functions in pancreatic cancer cells[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(2): 126-130. doi: 10.3969/j.issn.1006-0111.2018.02.007

Citation:

HUANG Jin, LIU Jingxue, ZENG Ying, YUAN Fang. Transient receptor potential type vanilloid 1 regulates EGFR related functions in pancreatic cancer cells[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(2): 126-130. doi: 10.3969/j.issn.1006-0111.2018.02.007

胰腺癌细胞中TRPV1受体参与EGFR介导的细胞功能研究

doi: 10.3969/j.issn.1006-0111.2018.02.007

1.
上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437;上海市浦东新区人民医院药剂科, 上海 201200
2.
上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437
3.
上海市浦东新区人民医院药剂科, 上海 201200

基金项目: 上海市卫生和计划生育委员会科研课题资助项目（201440461），浦东新区卫生系统学科带头人培养计划（PWRd2014-11）

Transient receptor potential type vanilloid 1 regulates EGFR related functions in pancreatic cancer cells

1.
Department Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201200, China;Department Pharmacy, Pudong New Aera People's Hospital, Shanghai 200437, China
2.
Department Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201200, China
3.
Department Pharmacy, Pudong New Aera People's Hospital, Shanghai 200437, China

摘要: 目的研究TRPV1受体的表达变化对表皮生长因子受体（EGFR）蛋白表达的调控作用，及其对相关细胞功能的影响。方法利用过表达技术和siRNA技术研究TRPV1表达变化对EGFR蛋白表达的影响，用CCK-8实验检测细胞的增殖，并用Real-time PCR实验对功能相关的癌基因Akt2和K-ras的表达进行检测。结果在胰腺癌细胞中TRPV1过表达或是受激动剂激活后，EGFR的蛋白含量下调；反之，干扰TRPV1表达会使EGFR的蛋白表达增加；过表达TRPV1的胰腺癌细胞其增殖速率减缓；过表达TRPV1的胰腺癌细胞中原癌基因Akt2和K-ras的表达明显受到抑制，反之则激活其表达。结论胰腺癌细胞中TRPV1的表达调控了EGFR的蛋白含量，并对与其功能密切相关的细胞增殖和癌基因表达也有调节作用。
- 胰腺癌细胞 /
- TRPV1 /
- 表皮生长因子受体 /
- 细胞增殖
Abstract: Objective To evaluate the regulation of the TRPV1 receptor on the expression of EGFR protein and the effects on related cell functions. Methods Human pancreatic cancer cells PANC-1 were cultured and treated by over-expression and siRNA. Cell proliferations were detected by CCK-8 experiment. RT-PCR test was used to detect the expression of oncogenes Akt2 and K-ras. Results EGFR protein was down-regulated by TRPV1 over-expression or by agonist activation in pancreatic cancer cells. EGFR protein was increased by the interference of TRPV1 expression. The proliferation rate of pancreatic cancer cells was decreased and Akt2, K-ras were significantly inhibited by TRPV1 over-expressing. Conclusion The expression of TRPV1 in pancreatic cancer cells regulated the EGFR protein content. The cell proliferation and oncogene expression were inhibited by TRPV1 over-expressing.
- transient receptor potential vanilloid 1 /
- EGFR /
- pancreatic cancer cells /
- cell proliferation

[1]	Siegel RL,Miller KD,Jemal A. Cancer statistics,2017[J]. CA Cancer J Clin,2017,67(1):7-30.
[2]	Miller KD,Siegel RL,Lin CC,et al. Cancer treatment and survivorship statistics,2016[J]. CA Cancer J Clin,2016,66(4):271-289.
[3]	王正航,周军,沈琳. 胰腺癌药物治疗的现状与困境[J]. 中华肿瘤杂志,2017,39(9):712-716.
[4]	Voets T,Droogmans G,Wissenbach U,et al. The principle of temperature-dependent gating in cold-and heat-sensitive TRP channels[J]. Nature,2004,430(7001):748-754.
[5]	Kym PR,Kort ME,Hutchins CW. Analgesic potential of TRPV1 antagonists[J]. Biochem Pharmacol,2009,78(3):211-216.
[6]	Wong GY,Gavva NR. Therapeutic potential of vanilloid receptor TRPV1 agonists and antagonists as analgesics:Recent advances and setbacks[J]. Brain Res Rev,2009,60(1):267-277.
[7]	Prevarskaya N,Zhang L,Barritt G. TRP channels in cancer[J]. Biochim Biophys Acta, 2007,1772(8):937-946.
[8]	Drewes AM,Krarup AL,Detlefsen S,et al. Pain in chronic pancreatitis:the role of neuropathic pain mechanisms[J]. Gut,2008,57(11):1616-1627.
[9]	Zheng L,Chen J,Ma Z,et al. Capsaicin enhances anti-proliferation efficacy of pirarubicin via activating TRPV1 and inhibiting PCNA nuclear translocation in 5637 cells[J]. Mol Med Rep,2016,13(1):881-887.
[10]	Wu TT,Peters AA,Tan PT,et al. Consequences of activating the calcium-permeable ion channel TRPV1 in breast cancer cells with regulated TRPV1 expression[J]. Cell Calcium,2014,56(2):59-67.
[11]	Miao X,Liu G,Xu X,et al. High expression of vanilloid receptor-1 is associated with better prognosis of patients with hepatocellular carcinoma[J]. Cancer Genet Cytogenet,2008,186(1):25-32.
[12]	Lazzeri M,Vannucchi MG,Spinelli M,et al. Transient receptor potential vanilloid type 1(TRPV1) expression changes from normal urothelium to transitional cell carcinoma of human bladder[J]. Eur Urol,2005,48(4):691-698.
[13]	Bode AM, Cho YY, Zheng D,et al. Transient receptor potential type vanilloid 1 suppresses skin carcinogenesis[J].Cancer Res,2009,69(3):905-913.

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胰腺癌细胞中TRPV1受体参与EGFR介导的细胞功能研究

doi: 10.3969/j.issn.1006-0111.2018.02.007

1. 上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437;上海市浦东新区人民医院药剂科, 上海 201200

2. 上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437

3. 上海市浦东新区人民医院药剂科, 上海 201200

基金项目: 上海市卫生和计划生育委员会科研课题资助项目（201440461），浦东新区卫生系统学科带头人培养计划（PWRd2014-11）

收稿日期: 2017-12-01
修回日期: 2018-01-04

关键词:

胰腺癌细胞 /

TRPV1 /

表皮生长因子受体 /

细胞增殖

摘要: 目的研究TRPV1受体的表达变化对表皮生长因子受体（EGFR）蛋白表达的调控作用，及其对相关细胞功能的影响。方法利用过表达技术和siRNA技术研究TRPV1表达变化对EGFR蛋白表达的影响，用CCK-8实验检测细胞的增殖，并用Real-time PCR实验对功能相关的癌基因Akt2和K-ras的表达进行检测。结果在胰腺癌细胞中TRPV1过表达或是受激动剂激活后，EGFR的蛋白含量下调；反之，干扰TRPV1表达会使EGFR的蛋白表达增加；过表达TRPV1的胰腺癌细胞其增殖速率减缓；过表达TRPV1的胰腺癌细胞中原癌基因Akt2和K-ras的表达明显受到抑制，反之则激活其表达。结论胰腺癌细胞中TRPV1的表达调控了EGFR的蛋白含量，并对与其功能密切相关的细胞增殖和癌基因表达也有调节作用。

English Abstract

Transient receptor potential type vanilloid 1 regulates EGFR related functions in pancreatic cancer cells

1. Department Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201200, China;Department Pharmacy, Pudong New Aera People's Hospital, Shanghai 200437, China

2. Department Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201200, China

3. Department Pharmacy, Pudong New Aera People's Hospital, Shanghai 200437, China

Received Date: 2017-12-01
Rev Recd Date: 2018-01-04

Keywords:

transient receptor potential vanilloid 1 /
EGFR /
pancreatic cancer cells /
cell proliferation

Abstract: Objective To evaluate the regulation of the TRPV1 receptor on the expression of EGFR protein and the effects on related cell functions. Methods Human pancreatic cancer cells PANC-1 were cultured and treated by over-expression and siRNA. Cell proliferations were detected by CCK-8 experiment. RT-PCR test was used to detect the expression of oncogenes Akt2 and K-ras. Results EGFR protein was down-regulated by TRPV1 over-expression or by agonist activation in pancreatic cancer cells. EGFR protein was increased by the interference of TRPV1 expression. The proliferation rate of pancreatic cancer cells was decreased and Akt2, K-ras were significantly inhibited by TRPV1 over-expressing. Conclusion The expression of TRPV1 in pancreatic cancer cells regulated the EGFR protein content. The cell proliferation and oncogene expression were inhibited by TRPV1 over-expressing.

黄瑾, 刘景雪, 曾颖, 袁芳. 胰腺癌细胞中TRPV1受体参与EGFR介导的细胞功能研究[J]. 药学实践与服务, 2018, 36(2): 126-130. doi: 10.3969/j.issn.1006-0111.2018.02.007

引用本文:

黄瑾, 刘景雪, 曾颖, 袁芳. 胰腺癌细胞中TRPV1受体参与EGFR介导的细胞功能研究[J]. 药学实践与服务, 2018, 36(2): 126-130. doi: 10.3969/j.issn.1006-0111.2018.02.007

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胰腺癌细胞中TRPV1受体参与EGFR介导的细胞功能研究

doi: 10.3969/j.issn.1006-0111.2018.02.007

Transient receptor potential type vanilloid 1 regulates EGFR related functions in pancreatic cancer cells

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胰腺癌细胞中TRPV1受体参与EGFR介导的细胞功能研究

doi: 10.3969/j.issn.1006-0111.2018.02.007

1. 上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437;上海市浦东新区人民医院药剂科, 上海 201200 2. 上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437 3. 上海市浦东新区人民医院药剂科, 上海 201200

English Abstract

Transient receptor potential type vanilloid 1 regulates EGFR related functions in pancreatic cancer cells

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目录

1. 上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437;上海市浦东新区人民医院药剂科, 上海 201200

2. 上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437

3. 上海市浦东新区人民医院药剂科, 上海 201200