灵芝真菌免疫调节蛋白在体外对不同胃癌细胞的抑制作用

许燕; 胡明慧; 高蓓蓉; 周选围

doi:10.3969/j.issn.1006-0111.2016.03.008

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灵芝真菌免疫调节蛋白在体外对不同胃癌细胞的抑制作用

许燕, 胡明慧, 高蓓蓉, 周选围

文章导航 > 药学实践与服务 > 2016 > 34(3): 223-226,277

许燕, 胡明慧, 高蓓蓉, 周选围. 灵芝真菌免疫调节蛋白在体外对不同胃癌细胞的抑制作用[J]. 药学实践与服务, 2016, 34(3): 223-226,277. doi: 10.3969/j.issn.1006-0111.2016.03.008

引用本文:

XU Yan, HU Minghui, GAO Beirong, ZHOU Xuanwei. Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 223-226,277. doi: 10.3969/j.issn.1006-0111.2016.03.008

Citation:

XU Yan, HU Minghui, GAO Beirong, ZHOU Xuanwei. Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 223-226,277. doi: 10.3969/j.issn.1006-0111.2016.03.008

灵芝真菌免疫调节蛋白在体外对不同胃癌细胞的抑制作用

doi: 10.3969/j.issn.1006-0111.2016.03.008

1.
阿斯利康中国创新中心, 上海 201203;复旦大学生命科学学院, 上海 200438
2.
阿斯利康中国创新中心, 上海 201203
3.
上海交通大学农业与生物学院, 上海 200240

基金项目: 国家自然科学基金面上项目(30771500)

Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro

1.
AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China;School of Life Sciences, Fudan University, Shanghai 200438, China
2.
AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China
3.
School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China

摘要: 目的通过体外试验测定了3种灵芝免疫调节蛋白(FIP-glu、FIP-gat和FIP-SN15)对26株人胃癌细胞抑制增殖的影响。方法采用MTS比色法检测了3种灵芝免疫调节蛋白对26株胃癌细胞的抑制作用,计算出每种灵芝免疫调节蛋白对不同胃癌细胞50%生长抑制所需的药物浓度值(GI₅₀)。结果灵芝免疫调节蛋白加入72 h后,发现3种真菌免疫调节蛋白分别对7株胃癌细胞株NUGC-3、GTL-16、HGC-27、IM95m、SNU-638、SNU-216和SNU-5表现出较强的抑制作用,其GI₅₀<50 μg/ml。只对2株胃癌细胞NUGC-4和OCUM-1没有抑制作用。结论灵芝免疫调节蛋白在体外对大多数人胃癌细胞株有较强的抑制作用,3种灵芝免疫调节蛋白的作用趋势相同,有进一步开发成抗胃癌药物的潜力。
- 灵芝 /
- 真菌免疫调节蛋白 /
- 胃癌细胞 /
- 细胞增殖 /
- 细胞毒性 /
- MTS比色法
Abstract: Objective To study the cell proliferative effects of fungal immunomodulatory proteins from Ganoderma spp. on 26 gastric cancer cell lines in vitro. Method 26 human gastric cancer cell lines were treated with FIPs by MTS assay. The average optical density(OD) in 490 nm and inhibition rate(GI₅₀)was counted by Universal Microplate Spectrophotometer. Results Three FIPs showed similar profiling in 26 human gastric cancer cell lines after 72 h treatment in cell proliferation assay, which except for NUGC-4 and OCUM-1 did not showed obvious anti-proliferative effect, the other 24 human gastric cell lines showed some anti-proliferative effects, especially for 7 cell lines(NUGC-3, GTL-16, HGC-27, IM95m, SNU-638, SNU-216 and SNU-5) showing strong potency, with their GI₅₀ less than 50 μg/ml. Conclusion FIPs showed strong anti-proliferative effects in some human gastric cancer cell lines in vitro, which had potential to be further developed as anti-gastric cancer drugs.
- Ganoderma spp. /
- fungal immunomodulatory proteins(FIPs) /
- gastric cancer cell lines /
- cell proliferation /
- cytotoxicity /
- MTS colorimetric assay

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灵芝真菌免疫调节蛋白在体外对不同胃癌细胞的抑制作用

doi: 10.3969/j.issn.1006-0111.2016.03.008

1. 阿斯利康中国创新中心, 上海 201203;复旦大学生命科学学院, 上海 200438

2. 阿斯利康中国创新中心, 上海 201203

3. 上海交通大学农业与生物学院, 上海 200240

基金项目: 国家自然科学基金面上项目(30771500)

收稿日期: 2015-06-26
修回日期: 2015-10-21

关键词:

灵芝 /

摘要: 目的通过体外试验测定了3种灵芝免疫调节蛋白(FIP-glu、FIP-gat和FIP-SN15)对26株人胃癌细胞抑制增殖的影响。方法采用MTS比色法检测了3种灵芝免疫调节蛋白对26株胃癌细胞的抑制作用,计算出每种灵芝免疫调节蛋白对不同胃癌细胞50%生长抑制所需的药物浓度值(GI₅₀)。结果灵芝免疫调节蛋白加入72 h后,发现3种真菌免疫调节蛋白分别对7株胃癌细胞株NUGC-3、GTL-16、HGC-27、IM95m、SNU-638、SNU-216和SNU-5表现出较强的抑制作用,其GI₅₀<50 μg/ml。只对2株胃癌细胞NUGC-4和OCUM-1没有抑制作用。结论灵芝免疫调节蛋白在体外对大多数人胃癌细胞株有较强的抑制作用,3种灵芝免疫调节蛋白的作用趋势相同,有进一步开发成抗胃癌药物的潜力。

English Abstract

Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro

1. AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China;School of Life Sciences, Fudan University, Shanghai 200438, China

2. AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China

3. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China

Received Date: 2015-06-26
Rev Recd Date: 2015-10-21

Keywords:

Ganoderma spp. /
fungal immunomodulatory proteins(FIPs) /
gastric cancer cell lines /
cell proliferation /
cytotoxicity /
MTS colorimetric assay

Abstract: Objective To study the cell proliferative effects of fungal immunomodulatory proteins from Ganoderma spp. on 26 gastric cancer cell lines in vitro. Method 26 human gastric cancer cell lines were treated with FIPs by MTS assay. The average optical density(OD) in 490 nm and inhibition rate(GI₅₀)was counted by Universal Microplate Spectrophotometer. Results Three FIPs showed similar profiling in 26 human gastric cancer cell lines after 72 h treatment in cell proliferation assay, which except for NUGC-4 and OCUM-1 did not showed obvious anti-proliferative effect, the other 24 human gastric cell lines showed some anti-proliferative effects, especially for 7 cell lines(NUGC-3, GTL-16, HGC-27, IM95m, SNU-638, SNU-216 and SNU-5) showing strong potency, with their GI₅₀ less than 50 μg/ml. Conclusion FIPs showed strong anti-proliferative effects in some human gastric cancer cell lines in vitro, which had potential to be further developed as anti-gastric cancer drugs.

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灵芝真菌免疫调节蛋白在体外对不同胃癌细胞的抑制作用

doi: 10.3969/j.issn.1006-0111.2016.03.008

Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro

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灵芝真菌免疫调节蛋白在体外对不同胃癌细胞的抑制作用

doi: 10.3969/j.issn.1006-0111.2016.03.008

1. 阿斯利康中国创新中心, 上海 201203;复旦大学生命科学学院, 上海 200438 2. 阿斯利康中国创新中心, 上海 201203 3. 上海交通大学农业与生物学院, 上海 200240

English Abstract

Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro

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目录

1. 阿斯利康中国创新中心, 上海 201203;复旦大学生命科学学院, 上海 200438

2. 阿斯利康中国创新中心, 上海 201203

3. 上海交通大学农业与生物学院, 上海 200240