大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究

韩燕; 闻俊; 周婷婷; 范国荣

doi:10.3969/j.issn.1006-0111.2015.03.012

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究

韩燕, 闻俊, 周婷婷, 范国荣

文章导航 > 药学实践与服务 > 2015 > 33(3): 238-241

韩燕, 闻俊, 周婷婷, 范国荣. 大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究[J]. 药学实践与服务, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012

引用本文:

韩燕, 闻俊, 周婷婷, 范国荣. 大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究[J]. 药学实践与服务, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012

HAN Yan, WEN Jun, ZHOU Tingting, FAN Guorong. The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012

Citation:

HAN Yan, WEN Jun, ZHOU Tingting, FAN Guorong. The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012

大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究

doi: 10.3969/j.issn.1006-0111.2015.03.012

1.
福建中医药大学药学院, 福建福州 350108
2.
第二军医大学药学院药物分析学教研室, 上海 200433
3.
福建中医药大学药学院, 福建福州 350108;第二军医大学药学院药物分析学教研室, 上海 200433

基金项目: 国家自然科学基金资助项目(30801541)

The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina

1.
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China
2.
School of Pharmacy, The Second Military Medical University, Shanghai 200433, China
3.
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;School of Pharmacy, The Second Military Medical University, Shanghai 200433, China

摘要: 目的研究大孔树脂分离纯化栀子豉汤有效成分的工艺条件及优化。方法采用紫外分光光度法测定总环烯醚萜苷和总大豆异黄酮的量,以总环烯醚萜苷和总大豆异黄酮的含量作为指标,采用单因素实验考察上样液浓度、上样量、洗脱剂浓度及用量等对分离纯化工艺的影响。结果最佳工艺条件:上样液最佳吸附浓度为0.1 g/ml(该浓度以每1 ml中所含生药材质量计),上样量与树脂体积比是2:1,吸附时间2 h,依次用1 BV(柱体积)水,6 BV 20%乙醇洗脱,6 BV 60%乙醇洗脱,收集洗脱液。结论该纯化工艺合理、稳定,可推广于工业生产。
- 栀子豉汤 /
- 大孔吸附树脂 /
- 分离纯化 /
- 总环烯醚萜苷 /
- 总大豆异黄酮
Abstract: Objective To optimize separation and purification technology of Zhizichi decoction by macroporous resina. Methods The content of total iridoid glycosides and total isoflavones were determined by ultraviolet spectrophotometry. Taking the content of total iridoid glycosides and total isoflavones as indexes, the effect of the concentration of sample solution, medicinal herbs on the amount of sample, concentration and volume of eluent was investigated by single factor test. Results The optimum separation and purification technology was as following: the concentration of sample solution was 0.1 g/ml, the volume ratio of resin to material drug was 2:1, the adsorption time was 2 h, and the sample was firstly eluted with 1 BV water, then 6 BV 20% ethanol and 60% ethanol, and the eluent was collected. Conclusion This optimized separation and purification technology was reasonable and stable, and it could be extended to large-scale production applications.
- Zhizichi decoction /
- macroporous absorption resin /
- separation and purification /
- total iridoid glycosides /
- total isoflavones

[1]	范越,田明,王秀海,等. 栀子豉汤临床和实验研究进展[J]. 中医药学报, 2010, 38(1): 118-119.
[2]	田义龙,赵静,任艳青, 等. 栀子豉汤对胰岛素抵抗的改善作用及机制研究[J]. 中药药理与临床, 2010,(6):5-7.
[3]	张超云,谢东霞. 栀子豉汤治疗抑郁症有效部位的筛选研究[J]. 中华中医药刊, 2011, 29(11): 2493-2494.
[4]	袁圣龙.栀子豉汤在心身疾病治疗中的应用[J]. 云南中医中药杂志, 2013, 34(6): 76-77.
[5]	杨军宣,赵成城,刘昊. 栀子环烯醚萜苷的大孔吸附树脂纯化工艺研究[J].中草药, 2012, 43(9): 1756-1759.
[6]	彭柳,范开静,熊清平. 大孔树脂分离纯化栀子提取液中栀子苷的工艺优选[J]. 中国实验方剂学杂志, 2012, 18(22): 20-24.
[7]	郭文勇,刘彬果,钟蕾, 等. 大孔树脂吸附层析法提取淡豆豉总异黄酮的研究[J]. 第二军医大学学报,2004, 25(9):1003-1034.
[8]	王虹玉,喇孝瑾,白素芬,等. 正交试验法优选栀子豉汤的水提工艺[J]. 中国实验方剂学杂志,2012,18(22): 36-38.

[1]	冯志惠, 邓仪卿, 叶冰, 安培, 张宏, 张海军. 雀梅藤石油醚提取物诱导三阴性乳腺癌细胞凋亡的实验研究 . 药学实践与服务, 2024, 42(6): 253-259. doi: 10.12206/j.issn.2097-2024.202311055
[2]	丁华敏, 郭羽晨, 秦春霞, 宋志兵, 孙莉莉. 消风止痒颗粒通过降低白三烯水平对小鼠特应性皮炎急性瘙痒的治疗作用研究 . 药学实践与服务, 2024, 42(5): 211-216. doi: 10.12206/j.issn.2097-2024.202306031
[3]	景凯, 杨慈荣, 张圳, 臧艺蓓, 刘霞. 黄芪甲苷衍生物治疗慢性心力衰竭小鼠的药效评价及作用机制研究 . 药学实践与服务, 2024, 42(5): 190-197. doi: 10.12206/j.issn.2097-2024.202310004
[4]	陈方剑, 骆锦前, 王志君, 胡叶帅, 孙煜昕, 宋洪杰. HPLC-MS/MS同时测定感冒安颗粒中5种黄酮成分的含量 . 药学实践与服务, 2024, 42(9): 402-406. doi: 10.12206/j.issn.2097-2024.202403030
[5]	岳春华, 贲永光, 王海桥. 基于NLRP1炎症小体探讨百合知母汤抗抑郁的作用机制 . 药学实践与服务, 2024, 42(8): 325-333. doi: 10.12206/j.issn.2097-2024.202401033
[6]	黄韵, 张正银, 金英, 郑怡菁, 李铁军, 孙莉莉. 耐碳青霉烯类肺炎克雷伯菌及大肠埃希菌临床分离株耐药性及耐药基因分析 . 药学实践与服务, 2024, 42(10): 439-444. doi: 10.12206/j.issn.2097-2024.202309059

点击查看大图

计量

文章访问数: 3399
HTML全文浏览量: 235
PDF下载量: 725
被引次数: 0

大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究

doi: 10.3969/j.issn.1006-0111.2015.03.012

1. 福建中医药大学药学院, 福建福州 350108

2. 第二军医大学药学院药物分析学教研室, 上海 200433

3. 福建中医药大学药学院, 福建福州 350108;第二军医大学药学院药物分析学教研室, 上海 200433

基金项目: 国家自然科学基金资助项目(30801541)

收稿日期: 2014-12-18
修回日期: 2015-01-28

关键词:

摘要: 目的研究大孔树脂分离纯化栀子豉汤有效成分的工艺条件及优化。方法采用紫外分光光度法测定总环烯醚萜苷和总大豆异黄酮的量,以总环烯醚萜苷和总大豆异黄酮的含量作为指标,采用单因素实验考察上样液浓度、上样量、洗脱剂浓度及用量等对分离纯化工艺的影响。结果最佳工艺条件:上样液最佳吸附浓度为0.1 g/ml(该浓度以每1 ml中所含生药材质量计),上样量与树脂体积比是2:1,吸附时间2 h,依次用1 BV(柱体积)水,6 BV 20%乙醇洗脱,6 BV 60%乙醇洗脱,收集洗脱液。结论该纯化工艺合理、稳定,可推广于工业生产。

English Abstract

The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina

1. College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China

2. School of Pharmacy, The Second Military Medical University, Shanghai 200433, China

3. College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;School of Pharmacy, The Second Military Medical University, Shanghai 200433, China

Received Date: 2014-12-18
Rev Recd Date: 2015-01-28

Keywords:

Abstract: Objective To optimize separation and purification technology of Zhizichi decoction by macroporous resina. Methods The content of total iridoid glycosides and total isoflavones were determined by ultraviolet spectrophotometry. Taking the content of total iridoid glycosides and total isoflavones as indexes, the effect of the concentration of sample solution, medicinal herbs on the amount of sample, concentration and volume of eluent was investigated by single factor test. Results The optimum separation and purification technology was as following: the concentration of sample solution was 0.1 g/ml, the volume ratio of resin to material drug was 2:1, the adsorption time was 2 h, and the sample was firstly eluted with 1 BV water, then 6 BV 20% ethanol and 60% ethanol, and the eluent was collected. Conclusion This optimized separation and purification technology was reasonable and stable, and it could be extended to large-scale production applications.

韩燕, 闻俊, 周婷婷, 范国荣. 大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究[J]. 药学实践与服务, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012

引用本文:

韩燕, 闻俊, 周婷婷, 范国荣. 大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究[J]. 药学实践与服务, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012

Citation:

HAN Yan, WEN Jun, ZHOU Tingting, FAN Guorong. The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012

全文HTML

参考文献 (8)

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

主管、主办: 中国人民解放军海军军医大学地址: 上海市杨浦区国和路325号邮政编码: 200433

电话: （021）81871324,81871397 E-mail: [email protected]

网站备案号沪ICP备05003363号-1

本系统由北京仁和汇智信息技术有限公司开发

留言板

大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究

doi: 10.3969/j.issn.1006-0111.2015.03.012

The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina

计量

出版历程

大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究

doi: 10.3969/j.issn.1006-0111.2015.03.012

1. 福建中医药大学药学院, 福建 福州 350108 2. 第二军医大学药学院药物分析学教研室, 上海 200433 3. 福建中医药大学药学院, 福建 福州 350108;第二军医大学药学院药物分析学教研室, 上海 200433

English Abstract

The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina

全文HTML

目录

1. 福建中医药大学药学院, 福建福州 350108

2. 第二军医大学药学院药物分析学教研室, 上海 200433

3. 福建中医药大学药学院, 福建福州 350108;第二军医大学药学院药物分析学教研室, 上海 200433