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双配体修饰聚合物泡囊的制备及细胞摄取

解方园,  俞媛,  耿雯倩,  钟延强

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文章导航 > 药学实践与服务  > 2015 >  33(1): 44-48
解方园, 俞媛, 耿雯倩, 钟延强. 双配体修饰聚合物泡囊的制备及细胞摄取[J]. 药学实践与服务, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
引用本文: 解方园, 俞媛, 耿雯倩, 钟延强. 双配体修饰聚合物泡囊的制备及细胞摄取[J]. 药学实践与服务, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
shu
XIE Fangyuan, YU Yuan, GENG Wenqian, ZHONG Yanqiang. The preparation and the cell uptake of polymer vesicles modified with dual ligands[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
Citation: XIE Fangyuan, YU Yuan, GENG Wenqian, ZHONG Yanqiang. The preparation and the cell uptake of polymer vesicles modified with dual ligands[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
shu

双配体修饰聚合物泡囊的制备及细胞摄取

doi: 10.3969/j.issn.1006-0111.2015.01.011

  • 第二军医大学药学院药剂学教研室, 上海 200433

基金项目: 国家自然科学基金(81302714);上海市科委纳米专项(11nm0504400)

The preparation and the cell uptake of polymer vesicles modified with dual ligands

  • Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • 摘要: 目的 构建一种主动靶向的新型纳米药物载体——聚合物泡囊(polymer vesicles,PVs),并考察其细胞摄取。 方法 以马来酰亚胺-聚乙二醇-聚乳酸-羟基乙酸共聚物(MAL-PEG-PLGA)为载体材料,通过自组装制备PVs,用转铁蛋白(Tf)与Tet-1对PVs进行修饰,构建纳米药物载体(Tf/Tet-1-PVs)。以香豆素-6作为荧光探针包载于药物载体,考察脑微血管内皮细胞(BCEC)及神经细胞(Neuro-2a)对载体系统的摄取。 结果 PVs粒径约80 nm,形态圆整,电镜观察具有明显膜层结构。BCEC细胞和Neuro-2a细胞对Tf/Tet-1-PVs的摄取均显著优于空白对照组和单配体修饰对照组。 结论 PVs经双配体Tf及Tet-1修饰后可促进脑微血管内皮细胞和神经细胞的摄取。
    Abstract: Objective To construct an active targeting drug delivery system- polymer vesicles(PVs),and examined the cellular uptake. Methods Maleimide-polyethylene glycol-poly(lactic-co-glycolic acid)(MAL-PEG-PLGA)was used as carrier materials to prepare PVs by self-assembling.And then PVs was modified by Tf and Tet-1(Tf/Tet-1-PVs).To evaluate its active targeting,coumarin-6 was used as a fluorescent probe to analyze cellular uptake of PVs for both BCEC and Neuro-2a cells. Results PVs was about 80 nm with rounded shape and had obvious film structure.Tf/Tet-1-PVs exhibited a significant role in promoting cellular uptake for both BCEC and Neuro-2a cells compared with control and single ligand-modified group. Conclusion PVs modified with dual ligands could promote the cell uptake for both brain capillary cells and nerve cells.
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  • 收稿日期:  2014-10-11
  • 修回日期:  2014-11-25

双配体修饰聚合物泡囊的制备及细胞摄取

doi: 10.3969/j.issn.1006-0111.2015.01.011
  • 第二军医大学药学院药剂学教研室, 上海 200433
    • 基金项目:  国家自然科学基金(81302714);上海市科委纳米专项(11nm0504400)
  • 收稿日期: 2014-10-11
  • 修回日期: 2014-11-25

摘要: 目的 构建一种主动靶向的新型纳米药物载体——聚合物泡囊(polymer vesicles,PVs),并考察其细胞摄取。 方法 以马来酰亚胺-聚乙二醇-聚乳酸-羟基乙酸共聚物(MAL-PEG-PLGA)为载体材料,通过自组装制备PVs,用转铁蛋白(Tf)与Tet-1对PVs进行修饰,构建纳米药物载体(Tf/Tet-1-PVs)。以香豆素-6作为荧光探针包载于药物载体,考察脑微血管内皮细胞(BCEC)及神经细胞(Neuro-2a)对载体系统的摄取。 结果 PVs粒径约80 nm,形态圆整,电镜观察具有明显膜层结构。BCEC细胞和Neuro-2a细胞对Tf/Tet-1-PVs的摄取均显著优于空白对照组和单配体修饰对照组。 结论 PVs经双配体Tf及Tet-1修饰后可促进脑微血管内皮细胞和神经细胞的摄取。

English Abstract

解方园, 俞媛, 耿雯倩, 钟延强. 双配体修饰聚合物泡囊的制备及细胞摄取[J]. 药学实践与服务, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
引用本文: 解方园, 俞媛, 耿雯倩, 钟延强. 双配体修饰聚合物泡囊的制备及细胞摄取[J]. 药学实践与服务, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
shu
XIE Fangyuan, YU Yuan, GENG Wenqian, ZHONG Yanqiang. The preparation and the cell uptake of polymer vesicles modified with dual ligands[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
Citation: XIE Fangyuan, YU Yuan, GENG Wenqian, ZHONG Yanqiang. The preparation and the cell uptake of polymer vesicles modified with dual ligands[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 44-48. doi: 10.3969/j.issn.1006-0111.2015.01.011
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