摘要:
目的 建立高效液相-蒸发光散色器法测定积雪草药材及配方颗粒剂中积雪草苷的含量。 方法 采用依利特Hypersil ODS C18柱(4.6 mmá250 mm,5 μm),流动相为乙腈-水(v/v=3:8),流速为0.6 ml/min。 结果 积雪草苷在23.2~580.0 μg/ml范围内呈良好的线性关系,r=0.999 6;日内和日间精密度分别为2.1%和3.6%;药材的加样回收率为95.2% (RSD=3.2%,n=6),配方颗粒的加样回收率为104.6% (RSD=1.0%,n=6);5个产地的药材中积雪草苷的含量分别为0.33%、0.52%、0.22%、0.28%和0.39%,5个批号的配方颗粒中积雪草苷的含量分别为0.40%、0.44%、0.39%、0.44%和0.42%。 结论 该分析方法简便,结果准确,适用于积雪草药材及配方颗粒剂的质量控制。
Abstract:
Objective To develop a method for determination of assiticoside in Centella asiatica and its formula particles. Methods Hypersil ODS C18 column was used(4.6 mmá250 mm,5 μm),withacetonitrile-water(v/v=3:8) as mobile phase, and a flow rate of 0.6 ml/min. Results The result showed that the calibration curve was linear in the range of 23.2~580.0 μg/ml for asiaticoside, intra-day and inter-day precision results were 2.1% and 3.6%, the average recovery of asiaticoside were 95.2%(RSD=3.2%, n=6) in centella asiatica and 104.6%(RSD=1.0%,n=6) in its formula particles. The contents of asiaticoside in 5 medicinal materials were 0.33%,0.52%,0.22%,0.28% and 0.39%;while in 5 formula particles were 0.40%,0.44%,0.39%,0.44% and 0.42%. Conclusions The method was simple and accurate for quality control of Centella asiatica and its formula particles.
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