Optimal selection of abstraction procedure for Xinshu Granula by orthogonal test
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摘要: 目的 优选心舒颗粒中的醇溶性成分的提取工艺。 方法 以干膏得率、丹参酮ⅡA含量为评价指标,选用L9(34)正交表进行实验。考察提取时间、提取次数、乙醇浓度、乙醇用量对干膏得率和丹参酮ⅡA含量的影响,以多指标综合评分法确定最佳工艺。 结果 确定加生药材10倍量的90%乙醇,回流1.5 h,提取2次为最佳方案。 结论 最佳工艺基本稳定可行。Abstract: Objective To study the alcohol-soluble component abstraction procedure of Xinshu Granula. Methods The optional extraction process was selected for the yields of extracts & Tanshinone ⅡA by using L9(34) orthogonal design.The effects of alcohol concentration,alcohol amount,reflux time and times of reflux were observed. Results The optimal extraction process was determined by adding 90% alcohol,10 times the amount of Chinese medicinal materials,reflux extracting 2 times,1.5 hours per time. Conclusions The selected processing method was stable and feasible.
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Key words:
- Xinshu Granula /
- tanshinone ⅡA /
- orthogonal test /
- abstraction procedure
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[1] 李红卫,荆晓红,骆 勤,等. 正交试验法优选乳病消片醇提工艺条件[J].中成药,2008,30(9):1395. [2] 范 彬,石晓峰,沈 薇,等. 安乳颗粒中丹参的乙醇提取试验研究[J]. 中国药事,2010,24(9):744.
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