摘要:
目的 建立耐力胶囊3号中人参皂苷Rg1、Re、Rb1 含量的测定方法。 方法 采用水饱和的正丁醇回流提取,HPLC法测定含量,以C18为固定相,以乙腈(A)和0.1%磷酸(B)进行梯度洗脱,检测波长203 nm,流速1 ml/min。 结果 人参皂苷Rg1进样量在0.2~1.0 μg(r=0.999 2)、人参皂苷Re进样量在0.8~4.0 μg(r=0.999 1)、人参皂苷Rb1进样量在2.0~10.0 μg(r=0.999 1)的范围呈良好线性关系,人参皂苷Rg1平均加样回收率为96.67%,RSD值为1.14%;人参皂苷Re平均加样回收率为100.52%,RSD值为1.18%;人参皂苷Rb1平均加样回收率为96.94%,RSD值为0.59%。 结论 该含量测定方法简便准确,重复性好,适用于耐力胶囊3号的质量控制
Abstract:
Objective To establish a method for the determination of ginsenoside Rg1、ginsenoside Re、ginsenoside Rb1 in Naili capsule Nov. 3. Methods HPLC with C18 column was revised to control the content of Radix Panax quinguefolium, the detective wavelength was set at 203 nm, reflux extraction with water saturation of n-butyl alcohol, true and false identification by TCL, the mobile phase was a linear gradient of acetonitrile(A) and 0.1% phosphate acid(B). Results Ginsenoside Rg1, Ginsenoside Re and Ginsenoside Rb1 showed the good linear relationships at the range of 0.2~1.0 μg (r=0.999 2), 0.8~4.0 μg (r=0.999 1), 2.0~10.0μg (r=0.999 1), respectively. Their recovery rates:Ginsenoside Rg1 average recovery was 96.67%, RSD=1.14%;Ginsenoside Re average recovery was 100.52%, RSD=1.18%;and Ginsenoside Rb1 average recovery was 96.94%, RSD=0.59%, respectively. Conclusion These methods were simple and accurate, and was repeatable, which could be used in the quality control of Naili capsule Nov. 3.
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