Content variation of four flavonoid glycosides in Hippophae rhamnoides L. leaves before and after fermentation assayed by RP-HPLC
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摘要: 目的 建立反向高效液相色谱（RP-HPLC）法比较沙棘叶发酵前后芦丁、异槲皮苷、水仙苷、异鼠李素-3-O-葡萄糖苷含量的变化。 方法 沙棘叶及其发酵茶水提液浓缩干燥后用70%乙醇溶解，分析采用HPLC法，Extend-C18色谱柱（4.6 mm×250 mm，5 μm），流动相为乙腈-0.1%甲酸水溶液，流速1 ml/min，检测波长为356 nm，柱温30℃。 结果 沙棘叶中芦丁含量较高。经过发酵后，除异槲皮苷含量略有升高外，异鼠李素-3-O-葡萄糖苷含量变化不大，芦丁、水仙苷含量均有所下降。上述4种成分在一定范围内呈现良好的线性关系，相关系数r均大于0.9997；精密度、重复性和稳定性良好；加样回收率在96%~103%之间。 结论 沙棘叶发酵降低了芦丁和水仙苷含量，增加了异槲皮苷含量。该法简便、可靠，可为沙棘叶和沙棘叶发酵茶的质量评价提供科学依据。Abstract: Objective To compare the changes of four flavonoid glycosides in the leaves of Hippophae rhamnoides L. before and after fermentation. Methods The water extract of Hippophae rhamnoides L. leaves and its fermented tea were concentrated and desiccated. The dry extracts were dissolved in 70% ethanol. The chromatographic separation was performed with RP-HPLC method on an Extend-C18 column (4.6 mm×250 mm, 5 μm). Acetonitrile-0.1% formic acid was selected as mobile phase at the flow rate of 1.0 ml/min. The detection wavelength was 356 nm and the column temperature was 30℃. Results The rutin content was high in the leaves of Hippophae rhamnoides L. After fermentation, isoquercitrin content was increased, while the contents of rutin and narcissoside were reduced and isorhamnetin-3-O-glucoside stayed unchanged. There was a good linear relationship between the concentration and peak areas of the four compounds (r>0.9997). The average recoveries were between 96%-103%. Conclusion This established method is rapid and reliable, which can be used for the quality control of Hippophae rhamnoides L. leaves and its fermented tea.
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Key words:
- RP-HPLC /
- Hippophae rhamnoides L. leave /
- fermentation /
- flavonoid glycosides
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[1] Li G, Du S, Guo K. Evaluation of limiting climatic factors and simulation of a climatically suitable habitat for Chinese Sea buckthorn[J]. PloS one, 2015, 10(7):e0131659. [2] 陈雏.青藏高原沙棘属植物资源与品质评价[D].成都:四川大学,2007. [3] Wagner H,Ulrich-Merzenich G. Evidence and Rational Based Research on Chinese Drugs[M]. Germany:Springer Vienna, 2013:181-236. [4] Ganju L, Padwad Y, Singh R, et al. Anti-inflammatory activity of Seabuckthorn (Hippophae rhamnoides) leaves[J]. Int Immunopharmacol, 2005, 5(12):1675-1684. [5] Yogendra Kumar MS, Tirpude RJ, Maheshwari DT, et al. Antioxidant and antimicrobial properties of phenolic rich fraction of Seabuckthorn (Hippophae rhamnoides L.) leaves in vitro[J]. Food Chem, 2013, 141(4):3443-3450. [6] Ni W, Gao T, Wang H, et al. Anti-fatigue activity of polysaccharides from the fruits of four Tibetan plateau indigenous medicinal plants[J]. J Ethnopharmacol, 2013, 150(2):529-535. [7] 国家药典委员会.中华人民共和国药典(2015年版)一部[S].北京:中国医药科技出版社,2015:184-185. [8] 李春英,王微,赵春建,等.沙棘不同部位总黄酮含量的测定及比较[J].植物研究,2005, 25(4):453-456. [9] 郁长治, 郑永刚, 吴旭东, 等. RP-HPLC法测定沙棘叶中黄酮苷的含量[J].中国当代医药, 2012, 19(11):51-52. [10] Zu Y, Li C, Fu Y, et al. Simultaneous determination of catechin, rutin, quercetin kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaves by RP-HPLC with DAD[J]. J Pharm Biomed Anal, 2006, 41(3):714-719. [11] 李坤, 王明芳, 薛非非, 等. 荷叶炒炭过程中金丝桃苷, 异槲皮苷与槲皮素含量变化规律研究[J]. 世界科学技术-中医药现代化, 2016(1):112-117. [12] 马志强,刘彬丽,刘璐,等.不同厂家舒血宁注射液中芦丁和水仙苷的HPLC含量测定[J].北京中医药大学学报,2013,36(11):768-770. [13] Barreca D, Laganà G, Leuzzi U, et al. Evaluation of the nutraceutical, antioxidant and cytoprotective properties of ripe pistachio (Pistacia vera L., variety Bronte) hulls[J]. Food Chem, 2016, 196:493-502. -
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