耳叶牛皮消中的苯乙酮类化合物及其抗氧化活性研究

陈炳阳; 岳荣彩; 刘芳; 李夏; 李元元; 吴国振; 单磊

doi:10.3969/j.issn.1006-0111.2013.05.008

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

耳叶牛皮消中的苯乙酮类化合物及其抗氧化活性研究

陈炳阳, 岳荣彩, 刘芳, 李夏, 李元元, 吴国振, 单磊

文章导航 > 药学实践与服务 > 2013 > 31(5): 351-354

陈炳阳, 岳荣彩, 刘芳, 李夏, 李元元, 吴国振, 单磊. 耳叶牛皮消中的苯乙酮类化合物及其抗氧化活性研究[J]. 药学实践与服务, 2013, 31(5): 351-354. doi: 10.3969/j.issn.1006-0111.2013.05.008

引用本文:

CHEN Bing-yang, YUE Rong-cai, LIU Fang, LI Xia, LI Yuan-yuan, WU Guo-zhen, SHAN Lei. Acetophenones and antioxidant activity in Cynanchum auriculatum Royle ex Wight[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(5): 351-354. doi: 10.3969/j.issn.1006-0111.2013.05.008

Citation:

CHEN Bing-yang, YUE Rong-cai, LIU Fang, LI Xia, LI Yuan-yuan, WU Guo-zhen, SHAN Lei. Acetophenones and antioxidant activity in Cynanchum auriculatum Royle ex Wight[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(5): 351-354. doi: 10.3969/j.issn.1006-0111.2013.05.008

耳叶牛皮消中的苯乙酮类化合物及其抗氧化活性研究

doi: 10.3969/j.issn.1006-0111.2013.05.008

第二军医大学药学院, 上海 200433

Acetophenones and antioxidant activity in Cynanchum auriculatum Royle ex Wight

School of Pharmacy, Second Military Medical University, Shanghai 200433, China

摘要: 目的研究耳叶牛皮消(萝藦科)Cynanchum auriculatum Royle ex Wight(asclepiadaceae)块根的化学成分及其抗氧化活性。方法药材提取物分部萃取后采用硅胶、Sephadex LH-20柱层析等进行分离纯化,通过理化性质和光谱分析鉴定结构。在H₂O₂刺激条件下,研究化合物对PC12细胞中超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性以及谷胱甘肽过氧化物酶(GSH-Px)活性的影响。结果从耳叶牛皮消中分离得到了5个苯乙酮类化合物,分别鉴定为cynandione A (I)、cynandione B (II)、cynandione C (III)、cynanchone A (IV)、cynantetrone (V)。H₂O₂能降低SOD、CAT和GSH-Px三种抗氧化酶活性,而耳叶牛皮消中的苯乙酮类化合物可抑制H₂O₂引起的氧化损伤。结论耳叶牛皮消中的苯乙酮类化合物具有抗氧化活性。
- 耳叶牛皮消 /
- 萝藦科 /
- 化学成分 /
- 苯乙酮类化合物
Abstract: Objective To study the chemical constituents and antioxidant activityof the root tubers of Cynanchum auriculatum Royle ex Wight (asclepiadaceae). Methods The root tubers were extracted with 95% EtOH, and the ethanol extract was further separated and purified by repeated column chromatography of silica gel and Sephadex LH-20. Structures of the compounds were identified on the basis of spectral analysis (including NMR and MS) and chemical evidences. Results Five acetophenones were isolated from Cynanchum auriculatum Royle ex Wight. Their structures were characterized as cynandione A (I), cynandione B (II), cynandione C (III), cynanchone A (IV), cynantetrone (V). H₂O₂ decreases SOD, CAT and GSH-Px activities, while the acetophenones could inhibit H₂O₂-induced oxidative damage. Conclusion The acetophenones isolated from the root tubers of Cynanchum auriculatum Royle ex Wight possessed potent antioxidant activities.
- Cynanchum auriculatum Royle ex Wight /
- asclepiadaceae /
- chemical constituents /
- acetophenones

[1]	State administration of traditional Chinese medicine of the People's Republic of China[M]. 'Zhong Hua Ben Cao', Shanghai Sci & Tech Press.Shanghai,1999,17,333.
[2]	Huang PL,Won SJ,Day SH,et al.A cytotoxic acetophenone with a novel skeleton, isolated from Cynanchum taiwanianum[J]. Helv Chim Acta.1999,82(10):1716.
[3]	Peng Y,Li Y,Liu X,et al.Antitumor activity of C-21 steroidal glycosides from Cynanchum auriculatum Royle ex Wight[J]. Phytomedicine, 2008,15(11):1016.
[4]	Shan L,Zhang WD,Zhang C,et al.Antitumor activity of crude extract and fractions from root tuber of Cynanchum auriculatum Royle ex Wight[J]. Phytother Res,2005,19(3):259.
[5]	Shan L,Liu RH,Shen YH,et al.Gastroprotective effect of a traditional Chinese herbal drug "Baishouwu" on experimental gastric lesions in rats[J].J Ethnopharmacol,2006,107(3):389.
[6]	Yue R,Yuan X,Liu X,et al.Cynandione A mitigates ischemic injuries in rats with cerebral ischemia[J].J Neurochem,2012,121:451.
[7]	Ji CX,Li XY,Jia SB,et al.The antidepressant effect of Cynanchum auriculatum in mice[J].Pharm Biol,2012,50(9):1067.
[8]	Liu CS,Chen NH,Zhang JT.Protection of PC12 cells from hydrogen peroxide-induced cytotoxicity by salvianolic acid B,a new compound isolated from Radix Salviae miltiorrhizae[J].Phytomedicine,2007,14: 492.
[9]	Lin CN, Huang PL,Lu CM,et al.Revised structure for five acetophenones from Cynanchum taiwanianum[J].Phytochemistry, 1997, 44(7): 1359.
[10]	Lin YL,Wu YM,Kuo YH.Revised structures for four acetophenones from Cynanchum taiwanianum[J].Phytochemistry,1997,45(5):1057.
[11]	Mathiesen L, Malterud KE, Sund RB. Hydrogen bond formation as basis for radical scavenging activity:a structure-activity study of C-methylated dihydrochalcones from Myrica gale and structurally related acetophenones[J].Free Radical Bio Med,1997,22:307.
[12]	Van den Worm E,Beukelman CJ,Van den Berg AJJ,et al.Effects of methoxylation of apocynin and analogs on the inhibition of reactive oxygen species production by stimulated human neutrophils[J].Eur J Pharmacol,2001,433:225.

[1]	宋雨桐, 夏德润, 顾珩, 唐少文, 易洪刚, 沃红梅. 帕博利珠单抗与铂类化疗方案在晚期非小细胞肺癌一线治疗中的药物经济学评价 . 药学实践与服务, 2024, 42(8): 334-340. doi: 10.12206/j.issn.2097-2024.202303023
[2]	冯志惠, 邓仪卿, 叶冰, 安培, 张宏, 张海军. 雀梅藤石油醚提取物诱导三阴性乳腺癌细胞凋亡的实验研究 . 药学实践与服务, 2024, 42(6): 253-259. doi: 10.12206/j.issn.2097-2024.202311055
[3]	毛智毅, 王筱燕, 陈晓颖, 汤逸斐. 度拉糖肽联合二甲双胍对肥胖型2型糖尿病患者机体代谢、体脂成分及血清脂肪因子的影响 . 药学实践与服务, 2024, 42(7): 305-309. doi: 10.12206/j.issn.2097-2024.202305032
[4]	陈静, 何瑞华, 翁月, 徐熠, 刘静, 黄瑾. 基于网络药理学和分子对接技术探究定清片活性成分治疗白血病的作用机制 . 药学实践与服务, 2024, 42(11): 479-486. doi: 10.12206/j.issn.2097-2024.202401073
[5]	陈方剑, 骆锦前, 王志君, 胡叶帅, 孙煜昕, 宋洪杰. HPLC-MS/MS同时测定感冒安颗粒中5种黄酮成分的含量 . 药学实践与服务, 2024, 42(9): 402-406. doi: 10.12206/j.issn.2097-2024.202403030
[6]	李丹, 戴贤春, 王芳珍, 陈奕含, 杨萍, 刘继勇. HPLC-MS/MS测定当归六黄汤中4种不同成分的含量 . 药学实践与服务, 2024, 42(6): 248-252, 266. doi: 10.12206/j.issn.2097-2024.202305007
[7]	丁华敏, 郭羽晨, 秦春霞, 宋志兵, 孙莉莉. 消风止痒颗粒通过降低白三烯水平对小鼠特应性皮炎急性瘙痒的治疗作用研究 . 药学实践与服务, 2024, 42(5): 211-216. doi: 10.12206/j.issn.2097-2024.202306031

点击查看大图

计量

文章访问数: 3343
HTML全文浏览量: 368
PDF下载量: 155
被引次数: 0

耳叶牛皮消中的苯乙酮类化合物及其抗氧化活性研究

doi: 10.3969/j.issn.1006-0111.2013.05.008

第二军医大学药学院, 上海 200433

收稿日期: 2012-12-04
修回日期: 2013-04-08

关键词:

耳叶牛皮消 /

萝藦科 /

化学成分 /

苯乙酮类化合物

摘要: 目的研究耳叶牛皮消(萝藦科)Cynanchum auriculatum Royle ex Wight(asclepiadaceae)块根的化学成分及其抗氧化活性。方法药材提取物分部萃取后采用硅胶、Sephadex LH-20柱层析等进行分离纯化,通过理化性质和光谱分析鉴定结构。在H₂O₂刺激条件下,研究化合物对PC12细胞中超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性以及谷胱甘肽过氧化物酶(GSH-Px)活性的影响。结果从耳叶牛皮消中分离得到了5个苯乙酮类化合物,分别鉴定为cynandione A (I)、cynandione B (II)、cynandione C (III)、cynanchone A (IV)、cynantetrone (V)。H₂O₂能降低SOD、CAT和GSH-Px三种抗氧化酶活性,而耳叶牛皮消中的苯乙酮类化合物可抑制H₂O₂引起的氧化损伤。结论耳叶牛皮消中的苯乙酮类化合物具有抗氧化活性。