长春花组织培养研究进展
Progress on tissue culture of Catharanthus roseus
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摘要: 长春花含有100多种吲哚类生物碱,具有抗肿瘤、降血压等多种生物活性,有较高的药用价值,但是含量偏低.长春花组织培养可从提高繁殖系数、调控次生代谢产物的累积等来提高长春花生物碱类成分含量.本文就外植体和培养基的选择、药用成分累积的影响因素、悬浮细胞培养应用、影响毛状根因素和基因工程技术应用等方面,综述了长春花组织培养的主要研究进展,为进一步开发利用长春花药物资源提供参考.Abstract: Catharanthus roseus (L.) G. Don contained more than 100 known indole alkaloids, some of which had anti-cancer, lowering blood pressure and other biological activity. Catharanthus roseus had a high medicinal value, but the content of alkaloids was very low. In order to improve the content of alkaloid, the technology of tissue culture had been applied in Catharanthus roseas,such as improving the propagation coefficient and controlling the accumulation of secondary metabolites. To provide reference in further development and utilization of Catharanthus roseus drug resources, the main research progress of tissue culture of Catharanthas roseus includingy the impact factors of medicinal components accumulation, suspension cell culture applications, the impact factors of hairy roots and application of genetic engineering technology were reviewed in this paper.
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Key words:
- Catharanthus roseu.s (L.) G. Don /
- tissue culture /
- secondary metabolites
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