Analysis of volatile constituents in Caulis Sinomenii by GC-MS
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摘要: 目的 建立气相色谱-质谱联用(GC-MS)方法分析青风藤中的挥发油成分。 方法 采用水蒸气蒸馏法提取青风藤挥发油,运用GC-MS技术,结合数据库检索对其挥发油进行分离鉴定。对青风藤中的挥发油进行分析。 结果 实验共鉴定出80个化合物,通过与软件中的质谱标准谱图库进行比较,获得匹配的有63个化合物,主要成分为脂肪酸、甾醇、烯烃以及少量的醛类等物质,含量最多的为十八烷酸和十六烷酸。 结论 该实验丰富了青风藤的物质基础研究,为青风藤质量控制提供了更多的参考依据。Abstract: Objective To establish the determination method for chemical constituents of the volatile oil in Sinornenium actum(Thunb.)Rehd.et Wils by gas chromatography-mass spectrometry(GC-MS). Method The volatile oil was extracted from Sinornenium actum(Thunb.)Rehd.et Wils by steam distillation.The constituents of volatile oil were identified by GC-MS technology,which were identified through database index. Results The results showed that after comparing of the gained mass spectrum with the standard, 63 kinds of chemical constituents in all were identified, the major constituents were the fatty acids, sterols, alkenes and a few kind of aldehyde. The most contents were octadecylic acid and hexadecanoic acid, whose relative percentages were 33.78% and 23.23% respectively. Conclusion The experiment improved the basic components study on Caulis Sinomenii, which could provide more reference quality control of Caulis Sinomenii.
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Key words:
- Sinornenium actum(Thunb.) /
- Rehd.et wils. /
- volatile oil
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