摘要:
目的: 建立复方二仙汤中盐酸小檗碱和总生物碱的含量测定方法。 方法: 小檗碱的含量测定应用Hypersil-BDS C18色谱柱,甲醇-0.02mol/L NaH2 PO4-0.1mol/L三乙胺（40:60:2）为流动相,检测波长为345nm;采用可见分光光度法于420nm波长处测定总生物碱的含量。 结果: 盐酸小檗碱的线性范围为0.51-10.10μg/mL,r=0.9999,回收率100.07%,RSD为0.4%;总生物碱线性范围为8.0-24.0μg/mL,r=0.9998,回收率100.46%,RSD为0.58%。 结论: 该法操作简便、快速且结果准确,可用于该复方的质量控制。
Abstract:
Objective: To establish a quantitative analysis method for berberine hydrochloride and total alkaloids in compound Er-xian decoction. Methods: Berberine hydrochloride were determined by HPLC. Using Hypersil-BDS C18 (4.6 x 200 mm) column, methanol-0.02 mol/L sodium dihydrogen phosphate-0.1 mol/L triethylamine (40-60-2) as the mobile phase. The detection wavelength was 345 nm. Total alkaloids were determined at 420 nm by visible spectrometry. Results: The linearity range of berberine hydrochloride was 0.51～10.10 μg/mL, r=0.999 9, and average recovery was 100.07%,RSD 0.4%. The linearity range of total alkaloids was 8.0～24.0 μg/mL, r=0.999 8, and average recovery was 100.46%, RSD 0.58%. Conclusions: The method is simple, quick and accurate.It can be used to control the quality of the decoction preparation.
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