摘要:
目的: 建立准确、快速测定罗哌卡因血药浓度的反相高效液相色谱法。 方法: 血样用乙醚和正己烷提取,布比卡因作内标,氮气吹干,流动相溶解进样。色谱条件:采用AgilentC18色谱柱（4.6 mm×150 mm,5μm）,流动相:0.045 mol/L磷酸二氢钾（pH3.0）-甲醇（55:45）,流速:1.0 mL/min,检测波长:210 nm,柱温:30℃。 结果: 罗哌卡因在0.250 9～5.018μg/mL范围内线性关系良好（r=0.999 6）,平均回收率102.0%,日内、日间精密度RSD均<15%,符合方法学要求。 结论: 本法快速、稳定,可用于罗哌卡因血药浓度监测。
Abstract:
Objective: To establish an accurate and rapid method for determination of ropivacaine by RP-HPLC. Methods: The sample was treated by the addition of bupivacaine as an internal standard,extraction with aether and n-hexane,and dryed by N2.The residue dissolved in the mobile phase and injected into chromatographic system.The HPLC system consisted of AgilentC18 column（4.6 mm×150 mm,5μm）and mobile phase of 0.045 mol/L KH2PO4（pH3.0）-methol（55:45）.Velocity of flow was 1.0 mL/min.The detection wavelength was 210 nm.Column temperature was 30℃. Results: The linear range was from 0.2509μg/mL to 5.018μg/mL with r of 0.999 6,the average recovery was 102.0%.RSD of Intra-day and Inter-day<15%.The results were accord with methodology. Conclusion: This method is quick and stable for ropivacaine monitoring in clinical research.
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