比色法测定逐瘀扶正胶囊中蒽醌类成分的含量
Determination of anthraquinones in Zhuyufuzheng capsules by colorimetry
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摘要: 目的 建立测定逐瘀扶正胶囊中蒽醌类成分含量的方法。方法 利用蒽醌类成分在碱性条件下显红色的特性,将经典的混合碱比色法加以改进后用于测定其含量。结果 改进后的比色法操作简便快捷,结果准确 (平均加样回收率为 99.86%),重复性好 (RSD=1.58%,n=5).可有效控制逐瘀扶正胶囊的质量。结论 所建立的混合碱比色法可作为逐瘀扶正胶囊中蒽醌类成分的定量方法,对于其它含有蒽醌类成分中药制剂的定量分析亦具有一定的方法学意义。Abstract: OBJECTIVE To establish a method to determinate the quantity of anthraquinones in Zhuyufuzheng capsule.METHODS Anthraquinones in Zhuyufuzheng capsules was determined by improved colorimetry.RESULTS This method was simple,quick and reproducible(RSD=1.58%, n=5).The average recovery was 99.86%.By this method,the quality of Zhuyufuzheng capsules can be controlled effectively.CONCLUSION The improved colorimetry can be used to determinate the quantity of anthraquinones in Zhuyufuzheng capsules.This method can be used for the quantitative analysis of other Chinese traditional medicines which contained anthraquinones as well.
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Key words:
- Anthraquinones /
- Colorimetry /
- Zhuyufuzheng capsules
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