HPLC法测定高原康胶囊中氨茶碱、地塞米松磷酸钠和地西泮3组分的含量
Determination of aminophylline dexamethasone sodium phosphate, diazepam in gaoyuankang capsules by HPLC
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摘要: 目的 建立同时测定高原康胶囊中氨茶碱、地塞米松磷酸钠、地西泮3组分含量的高效液相色谱法。方法 hypesil ODS柱,测定氨茶碱、地塞米松的流动相为甲醇- 0.05mol·L-1磷酸二氢钠溶液(55:45);测定地西泮的为甲醇-0.05mol·L-1磷酸二氢钠溶液(70:30)。流速 1.0ml·min-1,检测波长240nm.结果 线性范围:氨茶碱60～140μg·ml-1,r=0.9995;地塞米松磷酸钠4.8～11.2μg·ml-1,r=0.9993;地西泮 16.8～39.2μg·ml-1,r=0.9998.精密度及回收率结果良好。结论 本方法分离效果好,方法简便、快速,适用于该制剂3组分的同时测定。Abstract: To establish a method for determination of three constituents in Gaoyuankang capasules.METHODS A hypersil ODS column with a mobile phase composed of methol-phosphate buffer sulotion(55:45) was used.The detection wavelength was 240nm.RESULTS The linear response range was as follows aminophylline 60～140μg·ml-1(r=0.9995),dexamethasone sodium phosphate 4.8～11.2μg·ml-1(r=0.9993),diazepam 16.8～39.2μg·ml-1(r=0.9998),The recoveries of aminophylline,dexamethasone sodium phosphate,diazepam were 99.3%,99.4%,100.5%.CONCLUSIONS The method is simple,rapid and accurate.
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Key words:
- HPLC /
- aminophylline /
- dexamethasone sodium phosphate /
- diazepam
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