反相高效液相色谱法测定人血中利福平浓度
Determination of rifampicin concentration in human serum by RP-HPLC
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摘要: 目的 建立测定人血清中利福平浓度的高效液相色谱法(HPLC).方法 血清加4倍量的甲醇沉淀后离心,取上清液用0.45μ微孔滤膜滤过后进样。采用Nova-Pak C18柱,以甲醇-醋酸盐缓冲液(62:40)为流动相,氯氮作为内标,在334nm波长处测定。结果 利福平的线性范围为1～25μg/ml,平均回收率为(100.9±4.2)%,日内、日间RSD均小于4%.最低检测限为0.5μg/ml(信噪比≥3).结论 本法简便、可靠,适用于临床监测。Abstract: OBJECTIVE To determine the concentration of rifampicin in human serum by RP-HPLC.METHODS Serum was precipitated with centrifugation after add ed 4 times of methanol,and its super-stratum clear fluid was injected after filtration with 0.45μ micro-pore filter membrane.The method used Nova-Pak C18 column as chromatogram column,methanol-acetate buffer solution as flow phase,chlordiazepoxide as internal standard.The detection wavelength was 334nm.RESULTS The linear range of rifampicin was 1～25μg/ml.Average recovery was (100.9±4.2)%.Daily and daytime RSD were less than 4%.The lowest detection limit was 0.5μg/ml(the rate of signal and noise was greater than or equal to 3).CONCLUSION This method is simple,reliable and indicated for clinical monitoring.
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Key words:
- rifampicin /
- HPLC /
- blood drug concentration
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