Preparation and promoting blood circulation effect of Yantongping transdermal ointments

Key words: 
• Yantongping transdermal ointments /
• orthogonal design /
• promoting blood circulation

Abstract: Objective To find out the best prescription method for Yantongping transdermal ointments through orthogonal design and research on the effect of promoting blood circulation. Methods With 60 minutes in vitro cumulative penetration rate, using L9(3⁴) orthogonal experiment,the best formula and technology were found out through grinding method,and quality thereafter was evaluated. Mice was picked up as blank groups and model groups, each respectively was divided into six smaller groups as Vaseline, Vaseline and Fatty Alcohol.Yantongping transdermal ointments (low dosage),Yantongping transdermal ointments (medium dosage),Yantongping transdermal ointments (high dosage) and Voltaren Emulsions.△A and △V were measured before and after modeling by microcirculation method and microscopic micro-distance measuring method,while the blood circulation function of each medicine by the scoring of the blood of veinule was compared.Results The bestprescription was confirmed by A₂B₂C₃D₂.that was with the weight of the traditional Chinese drugs (TCD) Vaseline ratioes (A) to 50%,with the weight of TCD and Fatty Alcohol ratios (B) to 25%,the order of reagent was as follow:Fatty Alcohol,TCD,Vaseline.The grinding temperature was 80℃,and it passed five quality evaluation asphysical appearance,melting point,water value,stability,irritation.Before modeling,in the △A level measured,antongping group (low dosage) had statistical significance over Vaseline and Fatty Alcohol group in 30 min (P<0.05),while Yantongping group (medium dosage and high dosage) had obviously statistical significance over Vaseline and Fatty Alcohol group in different time periodsin the △A and△V level (P<0.01).In the blood flow quality evaluation measured,Yantongping group (low dosage) had statistical significance over Vaseline and Fatty Alcohol group in 20 min,30 min (P<0. 05); while Yantongping group (medium and high dosage) had obviously significant statistical significance over Vaseline and Fatty Alcohol group (P <0.01).After modeling, in the△A level, Yantongping group (low dosage) had statistical significance over Vaselineand and Fatty Alcohol group in 20 min ( P<0. 05);Yantongping group (low dosage) in 30 min, Yantongping group (medium and high dosage) had obviously significent statistical significance over Vaseline and Fatty Alcohol group in the△A and△V level. In the blood flow quality evaluation measured,Yantongping group (low,medium and high dosage) had obviously significant statistical significance over Vaseline and Fatty Alcohol group (P<0. 01).Conclusions Yantongping transdermal ointments prepared by the best prescription had a good function of promoting blood circulation.