Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 29 Issue 6
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2011  >  29(6): 457-459

CHEN Rui, CHU Zhong-ying, ZHANG Ji-chun, TIAN Lan. Determination of forbidden addition drugs of Diazepam,Clonazepam and Estazolam in tranquilizing mind traditional Chinese medicine by UPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 457-459.
Citation: CHEN Rui, CHU Zhong-ying, ZHANG Ji-chun, TIAN Lan. Determination of forbidden addition drugs of Diazepam,Clonazepam and Estazolam in tranquilizing mind traditional Chinese medicine by UPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 457-459.
shu

Determination of forbidden addition drugs of Diazepam,Clonazepam and Estazolam in tranquilizing mind traditional Chinese medicine by UPLC-MS/MS

  • Shanghai Songjiang Institute for Food and Drug Control, Shanghai 201600, China

  • Received Date: 2011-05-29
  • Rev Recd Date: 2011-06-15
  • Objective To establish a specific method for determination of forbidden addition drugs of Diazepam,Clonazepan and Estazolam in tranquilizing mind Traditional Chinese Medicine. Methods Diazepam, Clonazepam and Estazolam were determined and analyzed by UPLC-MS/MS. ACQUITY BEH C18 analysis column(2.1 mm×50 mm,1.7 μm) was used with the mobile phase of 10 mmol/L ammonium acetate and acetonitrile(90：10) with gradient elution. The flow rate was 0.4 ml/min,and the detection wavelength was 254 nm.EIS ion source was used with positive ion monitoring. Results The standard curves of Diazepam,Clonazepam and Estazolam showed good linearity over the concentration rang of 109.8~878.4, 102.1~816.8,106.9~855.2 ng/ml,The detection limits were 0.06,2.5×10-3,2.8×10-3 ng,the quantitation limits were 0.19,8.2×10-3,9.2×10-3 ng,the average recoveries of three chemicals was 95.7%~103.7%. Conclution The method was specific,sensitive,easily and quickly,which could be used to detect Diazepam,Clonazepan and Estazolam in tranquilizing mind traditional Chinese medicine.
  • [1] 李欣荣,陈安珍,杨 钊.UPLC-MS/MS检测消肿止痛类中成药中违禁添加的双氯芬酸钠和氨基比林[J].药物分析杂志,2010,30(7): 1294.
    [2] 国家食品药品监督管理局药品检验补充检验方法和检验项目批准件汇编[S].批准件编号2006008:109; 2006024:139.
  • 加载中
通讯作者: 陈斌, [email protected]
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(3569) PDF downloads(318) Cited by()

Related
Proportional views

Determination of forbidden addition drugs of Diazepam,Clonazepam and Estazolam in tranquilizing mind traditional Chinese medicine by UPLC-MS/MS

  • Shanghai Songjiang Institute for Food and Drug Control, Shanghai 201600, China
  • Received Date: 2011-05-29
  • Rev Recd Date: 2011-06-15

Abstract: Objective To establish a specific method for determination of forbidden addition drugs of Diazepam,Clonazepan and Estazolam in tranquilizing mind Traditional Chinese Medicine. Methods Diazepam, Clonazepam and Estazolam were determined and analyzed by UPLC-MS/MS. ACQUITY BEH C18 analysis column(2.1 mm×50 mm,1.7 μm) was used with the mobile phase of 10 mmol/L ammonium acetate and acetonitrile(90：10) with gradient elution. The flow rate was 0.4 ml/min,and the detection wavelength was 254 nm.EIS ion source was used with positive ion monitoring. Results The standard curves of Diazepam,Clonazepam and Estazolam showed good linearity over the concentration rang of 109.8~878.4, 102.1~816.8,106.9~855.2 ng/ml,The detection limits were 0.06,2.5×10-3,2.8×10-3 ng,the quantitation limits were 0.19,8.2×10-3,9.2×10-3 ng,the average recoveries of three chemicals was 95.7%~103.7%. Conclution The method was specific,sensitive,easily and quickly,which could be used to detect Diazepam,Clonazepan and Estazolam in tranquilizing mind traditional Chinese medicine.

CHEN Rui, CHU Zhong-ying, ZHANG Ji-chun, TIAN Lan. Determination of forbidden addition drugs of Diazepam,Clonazepam and Estazolam in tranquilizing mind traditional Chinese medicine by UPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 457-459.
Citation: CHEN Rui, CHU Zhong-ying, ZHANG Ji-chun, TIAN Lan. Determination of forbidden addition drugs of Diazepam,Clonazepam and Estazolam in tranquilizing mind traditional Chinese medicine by UPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(6): 457-459.
shu

    HTML

Reference (2)

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: [email protected]

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return