Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review,        editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
x Close Forever Close
Volume 29 Issue 5
. 
Turn off MathJax
Article Contents
Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2011  >  29(5): 379-381,400

LI Mao-xing, ZHANG Chao, WU Lan, WEI Li-li, QIN Jian-guo, ZHANG Quan-long, JIA Zheng-ping. Determination of phenylethanoid glycosides in Phlomis younghusbandii by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 379-381,400.
Citation: LI Mao-xing, ZHANG Chao, WU Lan, WEI Li-li, QIN Jian-guo, ZHANG Quan-long, JIA Zheng-ping. Determination of phenylethanoid glycosides in Phlomis younghusbandii by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 379-381,400.
shu

Determination of phenylethanoid glycosides in Phlomis younghusbandii by HPLC

  • 1.

    Department of Pharmacy, Lanzhou General Hospital of PLA, Key laboratory of the prevention and cure for the plateau environment damage, PLA, Lanzhou 730050, China;College of Life Science, Lanzhou University, Lanzhou 730000, China

  • 2.

    College of Chemical Engineering, Northwest University for Nationalities, Lanzhou 730030, China

  • 3.

    Department of Pharmacy, Lanzhou General Hospital of PLA, Key laboratory of the prevention and cure for the plateau environment damage, PLA, Lanzhou 730050, China

  • Received Date: 2011-02-23
  • Rev Recd Date: 2011-04-04
  • Objective To establish a HPLC method for the determination of three phenylethanoid glycosides in Phlomis younghusbandii. Methods HPLC analysis was performed on Symmetry C18 (4.6 mm×150 mm, 5 μm) column and the mixture of methanol-water (36:64, pH=4.5, HAc) was used as the mobile phase with a flow rate of 1.0 ml/min. The column temperature was 20 ℃ and the detection wavelength was 330 nm. Results The linear ranges of acteoside,alyssonoside,isoacteoside were 20~500(r=0.999 6), 10~130(r=0.999 5), 10~130 (r=0.999 7) μg/ml, respectively. The average recoveries (n=6) of them were all between 96% and 104%, RSD<5%. Conclusions This method was special, sensitive, accurate and with good repeatability, which could be used for quality control of Phlomis younghusbandii.
  • [1] 中国科学院中国植物志编辑委员会. 中国植物志( 65卷第二分册)[M]. 北京:化学工业出版社, 1995: 126,231.
    [2] 赵 斌, 董小萍, 吴健明. HPLC法测定螃蟹甲中山栀苷甲酯的含量[J]. 药物分析杂志,2009,29(2):323.
    [3] 张军莉, 徐 娟, 李茂星. HPLC法测定了藏药螃蟹甲中8-乙酰氧基山栀子苷甲酯的含量[J]. 解放军药学学报,2009,25(3):261.
    [4] 靖 会, 佐建锋, 李教社. 苯乙醇苷类化合物的药理研究进展[J]. 时珍国医国药, 2006, 17(3): 440.
    [5] 许敬英, 苏 奎, 周 静. 苯丙素苷类化合物的研究进展(Ⅱ)[J] .时珍国医国药, 2007, 18(7): 1770.
  • 加载中
通讯作者: 陈斌, [email protected]
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article Metrics

Article views(2249) PDF downloads(416) Cited by()

Related
Proportional views

Determination of phenylethanoid glycosides in Phlomis younghusbandii by HPLC

  • 1. Department of Pharmacy, Lanzhou General Hospital of PLA, Key laboratory of the prevention and cure for the plateau environment damage, PLA, Lanzhou 730050, China;College of Life Science, Lanzhou University, Lanzhou 730000, China
  • 2. College of Chemical Engineering, Northwest University for Nationalities, Lanzhou 730030, China
  • 3. Department of Pharmacy, Lanzhou General Hospital of PLA, Key laboratory of the prevention and cure for the plateau environment damage, PLA, Lanzhou 730050, China
  • Received Date: 2011-02-23
  • Rev Recd Date: 2011-04-04

Abstract: Objective To establish a HPLC method for the determination of three phenylethanoid glycosides in Phlomis younghusbandii. Methods HPLC analysis was performed on Symmetry C18 (4.6 mm×150 mm, 5 μm) column and the mixture of methanol-water (36:64, pH=4.5, HAc) was used as the mobile phase with a flow rate of 1.0 ml/min. The column temperature was 20 ℃ and the detection wavelength was 330 nm. Results The linear ranges of acteoside,alyssonoside,isoacteoside were 20~500(r=0.999 6), 10~130(r=0.999 5), 10~130 (r=0.999 7) μg/ml, respectively. The average recoveries (n=6) of them were all between 96% and 104%, RSD<5%. Conclusions This method was special, sensitive, accurate and with good repeatability, which could be used for quality control of Phlomis younghusbandii.

LI Mao-xing, ZHANG Chao, WU Lan, WEI Li-li, QIN Jian-guo, ZHANG Quan-long, JIA Zheng-ping. Determination of phenylethanoid glycosides in Phlomis younghusbandii by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 379-381,400.
Citation: LI Mao-xing, ZHANG Chao, WU Lan, WEI Li-li, QIN Jian-guo, ZHANG Quan-long, JIA Zheng-ping. Determination of phenylethanoid glycosides in Phlomis younghusbandii by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 379-381,400.
shu

    HTML

Reference (5)

Catalog

    版权所有：《药学实践与服务》编辑委员会

    Supervisor & Sponsors: Address: No 325 Guohe Road, ShanghaiPost Code: 200433

    Tel: （021）81871324,81871397 E-mail: [email protected]

    网站备案号 沪ICP备05003363号-1

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return