Analysis of chemical constituents of Lotus leaf by HPLC-TOF/MS

ZHOU Yong-gang; LIU Chang; MAO Fei; LI Xiang

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Objective To analyze chemical constituents of Lotus leaf by rapid-resolution (high performance) liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS). Methods A Agilent Zorbax SB-Aq column(250 mm×4.6mm,5μm) was used to separate. The mobile phase consisted of water containing 1.0 ％ ethylic acid and acetonitrile was used as gradient elute. The flow rate was 1.0 ml/min. TOF/MS was applied for qualitative analysis under positive ion mode. Results Under LC/MS condition, the major constituents of alkaloids and flavones in Lotus leaf were identified by time of flight mass spectrometry and structure-relevant fragment ions. Conclution The method of HPLC-TOF/MS was established to identify the chemical constituents of Lotus leaf which was simple and reliable.

Analysis of chemical constituents of Lotus leaf by HPLC-TOF/MS

1. Department of Pharmacy, 81st Hospital of PLA, Nanjing 210002, China

2. Agilent Ltd., Shanghai 200001, China

3. Department of Pharmacy, Beijing Children's Hospital, Beijing 100056, China

4. No. 62380 PLA troops, Beijing 100071, China

Received Date: 2011-06-09

Rev Recd Date: 2011-09-01

Key words:

Lotus leaf /
alkaloids /
flavones /
HPLC-TOF/MS

Abstract: Objective To analyze chemical constituents of Lotus leaf by rapid-resolution (high performance) liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS). Methods A Agilent Zorbax SB-Aq column(250 mm×4.6mm,5μm) was used to separate. The mobile phase consisted of water containing 1.0 ％ ethylic acid and acetonitrile was used as gradient elute. The flow rate was 1.0 ml/min. TOF/MS was applied for qualitative analysis under positive ion mode. Results Under LC/MS condition, the major constituents of alkaloids and flavones in Lotus leaf were identified by time of flight mass spectrometry and structure-relevant fragment ions. Conclution The method of HPLC-TOF/MS was established to identify the chemical constituents of Lotus leaf which was simple and reliable.

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Reference (14)

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Analysis of chemical constituents of Lotus leaf by HPLC-TOF/MS

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