Pharmacokinetics of levofloxacin in beagle dogs with open abdominal wound by different seawater immersion process

Key words: 
• levofloxacin /
• pharmacokinetics /
• open abdominal wound /
• seawater immersion /
• Beagle dogs

Abstract: Objective To compare the pharmacokinetics of levofloxacin(LVFX) between beagle dogs with open abdominal wound by seawater immersion for different time and normal dogs. Methods Twelve healthy beagle dogs were randomly divided into control group(A group,n=4),open abdominal wound and immersion for 45 min group(B group,n=4) and open abdominal wound and immersion for 90 min group(C group,n=4).The beagle dogs in B group and C group were immersed into artificial seawater for 45 or 90 min before administered,respectively.All the animal were administered a single dose of LVFX(25 mg/kg) by infusion into saphenous vein for 90 min.LVFX in plasma at different sampling time was determined by HPLC,at the same time,the temperature of animal was monitored.The pharmacokinetic parameters were calculated by 3P97 software and examined by SPSS 12.1 program. Results The temperature of animal in B and C group dropped significantly compared with A group in immersion process and after immersion,and these drops temperature were increased along with the extension of immersion process.The plasma concentration-time curve of three groups were fit to a two-compartment model with the main pharmacokinetic parameters as follows: C_max were(30.05±1.75),(36.10±4.74)and(42.34±4.19) mg/ L,t_1/2β were(8.23±0.65),(11.02±0.54) and(13.19±3.87) h,AUC were(247.00±14.10),(345.22±56.68) and(417.79± 83.32) mgoh/ L,Vc(6.97±2.02),(6.68±1.67) and(7.82±1.62) L,ClS(1.20±0.15),(1.06±0.12) and(0.92±0.15) L/ h,respectively.In comparison to A group,t_1/2β and AUC of B group was increased remarkably(P<0.05,respectively),and t_1/2β,C_max and AUC of C group was also increased remarkably,respectively.(t_1/2β,P<0.01;C_max,AUC,P<0.05).At the same time,there was sinificantly difference in t_1/2β between B group and C group(P<0.05). Conclusion Metabolism and elimination of LVFX in open abdominal wound by seawater immersion was obviously decreased when the immersion process was increased,and hypothermia in beagle that immersion led to might be invovled in these events.