Antibiotic tests of different polar extracts of Artemisia anomala in vitro

Key words: 
• Artemisia anomala /
• extracts /
• antibiotic test /
• minimal bacterial concentration(MBC)

Abstract: Objective The antibiotic tests of different polar extracts of Artemisia anomala S.Moore in vitro. Methods The antibiotic tests of different polar extracts of AASM in vitro were performed by using Kirby-Bauer method and broth dilution method. Results The minimal bactericidal concentration(MBC) of 80% ethyl alcohol extract of Artemisia anomala S.Moore is 25 mg/ml for Shigella dysenteriae.The MBC of chloroform extract is 6.25 mg/ml for Escherichia coli.The MBC of chloroform extract is 12.5 mg/ml for staphylococcus aureus.The MBC of acetic ether extract is 6.25 mg/ml for Shigella flexneri,Shigella dysenteriae and Streptococcus agalactiae.The MBC of acetic ether extract is 12.5 mg/ml for staphylococcus aureus.The MBC of butanol extract of is 12.5 mg/ml for Streptococcus agalactiae.The MBC of butanol extract is 25 mg/ml for Shigella dysenteriae.Petroleum ether extract did not show significant antibacterial activity. Conclusion Different polar parts of Artemisia anomala S.Moore on a variety of clinical pathogenic bacteria showed good bactericidal effect in vitro.Thus,at the anti-infective therapeutic areas there are great prospects for development and application.The effective monomer of Artemisia anomala and its bactericidal mechanism needs further study.