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Volume 27 Issue 6
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2009  >  27(6): 455-458,469

SUN Zhi-guo, ZHANG Li, TIAN Jing-kui, ZHONG yan-qiang, LU Ying. Studies on purification process of steroidal saponins from Paris polyphylla[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 455-458,469.
Citation: SUN Zhi-guo, ZHANG Li, TIAN Jing-kui, ZHONG yan-qiang, LU Ying. Studies on purification process of steroidal saponins from Paris polyphylla[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 455-458,469.
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Studies on purification process of steroidal saponins from Paris polyphylla

  • 1.

    Zhejiang University, Hongzhou 310028, China; College of Pharmacy, Second Military Medical Unversity, Shanghai 200433, China

  • 2.

    Zhejiang University, Hongzhou 310028, China

  • 3.

    College of Pharmacy, Second Military Medical Unversity, Shanghai 200433, China

  • Received Date: 2009-07-08
  • Objective :To optimize the purification process of saponins from Paris polyphylla Smith. Methods :According to the purity and gaining rate of total saponins,the optimum purifying process was obtained by a combination of adjusted the pH in alcohol solution,alcohol extracting-water precipitating and macroporous resin techniques. Results :The optimum purification conditions were obtained as follows:70% alcohol solution,the pH of which was adjusted to 9,was suckingly filtrated.After the pH to 7,the filtrate was concentrated to 0.5 g/mL and then centrifuged(4 000 r/min,30 min).The clear supernatant liquid was purified by D101 macroporous resin(loading sample volume:70% adsorption capacity,column diameter vs height:15,loading flow rate:4.5 BV/h,purification solvent:20% alcohol,volume:6 BV,eluting solvent:70% alcohol,volume:8 BV,eluting flow rate:8 BV/h).The purity and gaining rate of total saponins prepared by the optimum process were 71.6% and 85.71% respectively. Conclusion :The purification process of saponins obtained by this experiment is steady,reasonable and feasible.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Studies on purification process of steroidal saponins from Paris polyphylla

  • 1. Zhejiang University, Hongzhou 310028, China; College of Pharmacy, Second Military Medical Unversity, Shanghai 200433, China
  • 2. Zhejiang University, Hongzhou 310028, China
  • 3. College of Pharmacy, Second Military Medical Unversity, Shanghai 200433, China
  • Received Date: 2009-07-08

Abstract: Objective :To optimize the purification process of saponins from Paris polyphylla Smith. Methods :According to the purity and gaining rate of total saponins,the optimum purifying process was obtained by a combination of adjusted the pH in alcohol solution,alcohol extracting-water precipitating and macroporous resin techniques. Results :The optimum purification conditions were obtained as follows:70% alcohol solution,the pH of which was adjusted to 9,was suckingly filtrated.After the pH to 7,the filtrate was concentrated to 0.5 g/mL and then centrifuged(4 000 r/min,30 min).The clear supernatant liquid was purified by D101 macroporous resin(loading sample volume:70% adsorption capacity,column diameter vs height:15,loading flow rate:4.5 BV/h,purification solvent:20% alcohol,volume:6 BV,eluting solvent:70% alcohol,volume:8 BV,eluting flow rate:8 BV/h).The purity and gaining rate of total saponins prepared by the optimum process were 71.6% and 85.71% respectively. Conclusion :The purification process of saponins obtained by this experiment is steady,reasonable and feasible.

SUN Zhi-guo, ZHANG Li, TIAN Jing-kui, ZHONG yan-qiang, LU Ying. Studies on purification process of steroidal saponins from Paris polyphylla[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 455-458,469.
Citation: SUN Zhi-guo, ZHANG Li, TIAN Jing-kui, ZHONG yan-qiang, LU Ying. Studies on purification process of steroidal saponins from Paris polyphylla[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 455-458,469.
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