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Volume 27 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2009  >  27(4): 284-285,288

XIA Ai-jun, HUANG He-zhou, GAO Gang, ZHANG Hai, ZHU Zhen-yu, CHAI Yi-feng. Simultaneous determination of five flavone constituents in Epimedium Brevicornum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 284-285,288.
Citation: XIA Ai-jun, HUANG He-zhou, GAO Gang, ZHANG Hai, ZHU Zhen-yu, CHAI Yi-feng. Simultaneous determination of five flavone constituents in Epimedium Brevicornum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 284-285,288.
shu

Simultaneous determination of five flavone constituents in Epimedium Brevicornum by HPLC

  • 1.

    Department of pharmacy, 303th Hospital of PLA, Nanning 530021, China

  • 2.

    School of pharmacy, Second Military Medical University, Shanghai 200433, China

  • 3.

    Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200433, China

  • Received Date: 2008-11-20
  • Objective : To establish a HPLC method for simultaneous determination of five flavone constituents in Epimedium Brevicornum. Methods :The determination was performed on Diamonsil-C18 column(4.6mm×250mm,5μm,Dikma).The mobile phase was consisted of acetonitrile-formic acid aqueous solution with a flow rate of 0.9 mL/min.The detection wavelength was set at 270 nm. Results :A method has been established for determination of epimedin A1,epimedin B,icariin,sagittatoside A,baohuoside I in one run by HPLC. Conclusion :The established method is simple,convenient,accurate and reliable,and suitable for the quality control of Epimedium Brevicornum.
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  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

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Simultaneous determination of five flavone constituents in Epimedium Brevicornum by HPLC

  • 1. Department of pharmacy, 303th Hospital of PLA, Nanning 530021, China
  • 2. School of pharmacy, Second Military Medical University, Shanghai 200433, China
  • 3. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200433, China
  • Received Date: 2008-11-20

Abstract: Objective : To establish a HPLC method for simultaneous determination of five flavone constituents in Epimedium Brevicornum. Methods :The determination was performed on Diamonsil-C18 column(4.6mm×250mm,5μm,Dikma).The mobile phase was consisted of acetonitrile-formic acid aqueous solution with a flow rate of 0.9 mL/min.The detection wavelength was set at 270 nm. Results :A method has been established for determination of epimedin A1,epimedin B,icariin,sagittatoside A,baohuoside I in one run by HPLC. Conclusion :The established method is simple,convenient,accurate and reliable,and suitable for the quality control of Epimedium Brevicornum.

XIA Ai-jun, HUANG He-zhou, GAO Gang, ZHANG Hai, ZHU Zhen-yu, CHAI Yi-feng. Simultaneous determination of five flavone constituents in Epimedium Brevicornum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 284-285,288.
Citation: XIA Ai-jun, HUANG He-zhou, GAO Gang, ZHANG Hai, ZHU Zhen-yu, CHAI Yi-feng. Simultaneous determination of five flavone constituents in Epimedium Brevicornum by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(4): 284-285,288.
shu

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