Determination astragaloside Ⅳ in Radix Astragali by HPLC-ELSD

Key words: 
• HPLC-ELSD /
• Radix Astragali /
• astragalosrde Ⅳ /
• determination

Abstract: Objective To determine astragaloside Ⅳ in Radix Astragali by HPLC-KLSD. Methods Radix Astragali was extracted with MeOH-NH₃·H₂O(v/v 9:1),the condition was confirmed and the validation of the method was also tested. The chromatogra phy condition was with Hypersil ODS 2 column (4.6mm×250mm,5μm); mobile phase was A; ACN,B:H₂O, gradient elutron,flow speed was 1.0 ml/min, temperature of column was room temperature, inject volume:20μL. The KLSD conditions were as follows:the temperature of drift tube was 40℃, the gas pressure was 3.5Bar, the value of gain was 7. Results The intra-day and mter-day precision (RSD)at low, middle and high injection amount were all less than 2.0%. The stability (RSD) was 1.24% in 48h. The recuerence (RSD, n=5) was 1.26%. The limit of detection was 0.6930mg/mL The recoveries were 97.05% (KSD=0.17%, n=3) for astragaloside Ⅳ. The contents of astragaloside Ⅳ in (en different batch of Astragalus membranaceus (Fisch.) Bge. were accord to the standard of Chinese Pharmacopoeia. Conclusion The method covild shorten the process and time of Radix Astragali pretreatment with character of simple, suitable and reliable. The studies could apply to determine the content of astragaloside Ⅳ in Radix Astragalt.