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Volume 21 Issue 3
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2003  >  (3): 153-155,170

ZHANG Ming-qiong, ZHEN Hong-hui. Detecting bacterial endotoxin in procaine hydrochloride injection by limulus test after excluding interferentail factor by extraction[J]. Journal of Pharmaceutical Practice and Service, 2003, (3): 153-155,170.
Citation: ZHANG Ming-qiong, ZHEN Hong-hui. Detecting bacterial endotoxin in procaine hydrochloride injection by limulus test after excluding interferentail factor by extraction[J]. Journal of Pharmaceutical Practice and Service, 2003, (3): 153-155,170.
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Detecting bacterial endotoxin in procaine hydrochloride injection by limulus test after excluding interferentail factor by extraction

  • The 175th hospital of PLA, Zhangzhou 363000, China

  • Received Date: 2002-11-25
  • OBJECTIVE To establish a method of detecting bacterial endotoxin(BET)in procaine hydrochlo-ride injection, and to explore the feasibility of excluding interferentail factor of limulus test by extraction.METHODS ; After sodium hydroxide changed procaine hydrochloride into procaine,and chloroform extracted procaine limulus test was performed. RESULTS 15 batchs of procaine hydrochloride injection were detected. The quantity of BET in each batch was less than 0.25EU·mL-1 and their BET pollution degree were described truly. CONCLUSION Extraction excluding the interferentail factors of limulus test could widen range of application of BET exammatron.
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通讯作者: 陈斌, [email protected]
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Detecting bacterial endotoxin in procaine hydrochloride injection by limulus test after excluding interferentail factor by extraction

  • The 175th hospital of PLA, Zhangzhou 363000, China
  • Received Date: 2002-11-25

Abstract: OBJECTIVE To establish a method of detecting bacterial endotoxin(BET)in procaine hydrochlo-ride injection, and to explore the feasibility of excluding interferentail factor of limulus test by extraction.METHODS ; After sodium hydroxide changed procaine hydrochloride into procaine,and chloroform extracted procaine limulus test was performed. RESULTS 15 batchs of procaine hydrochloride injection were detected. The quantity of BET in each batch was less than 0.25EU·mL-1 and their BET pollution degree were described truly. CONCLUSION Extraction excluding the interferentail factors of limulus test could widen range of application of BET exammatron.

ZHANG Ming-qiong, ZHEN Hong-hui. Detecting bacterial endotoxin in procaine hydrochloride injection by limulus test after excluding interferentail factor by extraction[J]. Journal of Pharmaceutical Practice and Service, 2003, (3): 153-155,170.
Citation: ZHANG Ming-qiong, ZHEN Hong-hui. Detecting bacterial endotoxin in procaine hydrochloride injection by limulus test after excluding interferentail factor by extraction[J]. Journal of Pharmaceutical Practice and Service, 2003, (3): 153-155,170.
shu

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