Determination of amlodipine in serum by HPLC

Key words: 
• amlodipine /
• HPLC /
• the determination of conten

Abstract: A rapid specific and sensitive HPLC menthod for the determination of amlodipine in serum was developed, using a stainless steel column (ods-5s, 150×4.0mm) with nifedipine as internal standard. The mobile phase was a mixture of methanol-KH₂PO₄ buffer(0.03mol/L), flow rate 1ml/min. Wavelength of the detector was set at 300nm. The linear correlattion was observed within the range of 4-12μg/ml(r=0.9992) and detect limitation at 4ng. The mean recoveries of the method were 100.24±5.4O% (intra-day)and 99.87±6.23% (inter-day).