HPLC determination of caffeine metabolites in urine

Yi Tao; Lu Jianfen

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code

x Close Forever Close

PDF
Cite
Share

facebook

twitter

google

linkedin

Volume 15 Issue 2

Turn off MathJax

Article Contents

Article Navigation > Journal of Pharmaceutical Practice and Service > 1997 > (2): 94-99

Yi Tao, Lu Jianfen. HPLC determination of caffeine metabolites in urine[J]. Journal of Pharmaceutical Practice and Service, 1997, (2): 94-99.

Citation:

Yi Tao, Lu Jianfen. HPLC determination of caffeine metabolites in urine[J]. Journal of Pharmaceutical Practice and Service, 1997, (2): 94-99.

HPLC determination of caffeine metabolites in urine

Yi Tao¹,
Lu Jianfen²

1.
Wuhan General Hospital of PLA Wuhan 430070
2.

Abstract

A HPLC method was developed for determination of caffeine metabolites in urine(AFMU, 1X, 1U, 17X, 17U).The samplb added ammonium sulfate was extracted twice by a mixture of chloroform-isopropanol(85:15 vol/vol)and evaporated to dryness with a gentle stream ofair below)25℃.HPLC analysis was carried out on Shim Pack C₁₈ column with methanol-ace-tonitrile-0.05% acetic acid (10:1:89 vol/vol) as the mobile phase and UV detection at 280nm.The assay procedure has been applied to determining urinary metabolites excreted after oral caffeine in a healthy sample(n = 120).Caffeine metabolite ratios were used to assess the poymorphicN-acetyltransferase and CYP₁A₂ activities.
- Caffeine,
- HPLC,
- N-acetyltransferase,
- CYP₁A₂
References
Proportional views

通讯作者: 陈斌, [email protected]

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article Metrics

Article views(3538) PDF downloads(442) Cited by()

Proportional views