The C2min aptamer-modified gene delivery system for targeting ADPC/AIPC prostate cancer

ZHANG Jing; GU Yongwei; WU Xin

doi:10.3969/j.issn.1006-0111.201906038

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name

E-mail

Phone

Title

Content

Verification Code

Objective To synthesize a novel prostate cancer targeting gene vector PAMAM-PEG-C2min and improve gene transfection efficiency targeting on prostate cancer. Methods The aptamer (C2min) and polyamide-amine (PAMAM) were ligated by polyethylene glycol (PEG). The structure of the synthesized PAMAM-PEG-C2min was identified by NMR. The biological characteristics of the nanoparticles were examined by the uptake experiments and gene transfection experiments (the loaded gene was siR-M) with the prostate cancer cells (PC3 and LNCaP). Besides, the in vivo targeting was investigated using in vivo image system. The in vivo targeting results indicated that PAMAM-PEG-C2min can achieve the simultaneous targeting of two prostate cancer tissues. Results The PAMAM-PEG-C2min synthesis was confirmed by NMR. Cell uptake experiments showed that the cell uptake efficiency of PAMAM-PEG-C2min was concentration dependent. In vitro experiments showed that the PC3 and LNCaP cells transfection efficiency and targeting of PAMAM-PEG modified with C2min were significantly improved compared with the PEG modified PAMAM. Conclusion PAMAM-PEG-C2min is a potential targeted drug delivery vehicle. It provides a new technology platform for comprehensive and specific targeting treatment of prostate cancer.

The C2min aptamer-modified gene delivery system for targeting ADPC/AIPC prostate cancer

1. Department of Pharmacy, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, China

2. Shanghai Wei Er Laboratory, shanghai 201712, China

3. Department of Pharmacy, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, China;Shanghai Wei Er Laboratory, shanghai 201712, China

Received Date: 2019-06-14

Rev Recd Date: 2019-07-26

Key words:

Abstract: Objective To synthesize a novel prostate cancer targeting gene vector PAMAM-PEG-C2min and improve gene transfection efficiency targeting on prostate cancer. Methods The aptamer (C2min) and polyamide-amine (PAMAM) were ligated by polyethylene glycol (PEG). The structure of the synthesized PAMAM-PEG-C2min was identified by NMR. The biological characteristics of the nanoparticles were examined by the uptake experiments and gene transfection experiments (the loaded gene was siR-M) with the prostate cancer cells (PC3 and LNCaP). Besides, the in vivo targeting was investigated using in vivo image system. The in vivo targeting results indicated that PAMAM-PEG-C2min can achieve the simultaneous targeting of two prostate cancer tissues. Results The PAMAM-PEG-C2min synthesis was confirmed by NMR. Cell uptake experiments showed that the cell uptake efficiency of PAMAM-PEG-C2min was concentration dependent. In vitro experiments showed that the PC3 and LNCaP cells transfection efficiency and targeting of PAMAM-PEG modified with C2min were significantly improved compared with the PEG modified PAMAM. Conclusion PAMAM-PEG-C2min is a potential targeted drug delivery vehicle. It provides a new technology platform for comprehensive and specific targeting treatment of prostate cancer.

HTML

Reference (15)

[1]	SIEGEL R L, MILLER K D, JEMAL A. Cancer statistics, 2019[J]. CA Cancer J Clin,2019,69(1):7-34
[2]	郑保良, 孙国庆. 激素敏感转移性前列腺癌的临床预后因素研究[J]. 实用癌症杂志, 2018, 33(6):995-997, 1017
[3]	DEN R B, GEORGE D, PIECZONKA C, et al. Ra-223 treatment for bone metastases in castrate-resistant prostate cancer: practical management issues for patient selection[J]. Am J Clin Oncol,2019,42(4):399-406
[4]	EL-AMM J, ARAGON-CHING J B. The changing landscape in the treatment of metastatic castration-resistant prostate cancer[J]. Ther Adv Med Oncol,2013,5(1):25-40
[5]	WU X, DING B Y, GAO J, et al. Second-generation aptamer-conjugated PSMA-targeted delivery system for prostate cancer therapy[J]. Int J Nanomedicine,2011,6:1747-1756
[6]	WILNER S E, WENGERTER B, MAIER K, et al. An RNA alternative to human transferrin: a new tool for targeting human cells[J]. Mol Ther Nucleic Acids,2012,1:e21
[7]	KELLY J M, AMOR-COARASA A, PONNALA S, et al. Albumin-binding PSMA ligands: implications for expanding the therapeutic window[J]. J Nucl Med,2019,60(5):656-663
[8]	HINSON D L, WEBBER R J. Miniaturization of the BCA protein assay[J]. BioTechniques,1988,6(1):14, 16, 19
[9]	NI X, CASTANARES M, MUKHERJEE A, et al. Nucleic acid aptamers: clinical applications and promising new horizons[J]. Curr Med Chem,2011,18(27):4206-4214
[10]	CHANG Y M, DONOVAN M J, TAN W H. Using aptamers for cancer biomarker discovery[J]. J Nucleic Acids,2013,2013:817350
[11]	CHUNG C H, KIM J H, JUNG J, et al. Nuclease-resistant DNA aptamer on gold nanoparticles for the simultaneous detection of Pb²⁺ and Hg²⁺ in human serum[J]. Biosens Bioelectron,2013,41:827-832
[12]	HUANG Y Z, HERNANDEZ F J, GU B, et al. RNA aptamer-based functional ligands of the neurotrophin receptor, TrkB[J]. Mol Pharmacol,2012,82(4):623-635
[13]	SUNDARAM P, KURNIAWAN H, BYRNE M E, et al. Therapeutic RNA aptamers in clinical trials[J]. Eur J Pharm Sci,2013,48(1-2):259-271
[14]	PALMERSTON MENDES L, PAN J Y, TORCHILIN V P. Dendrimers as nanocarriers for nucleic acid and drug delivery in cancer therapy[J]. Molecules,2017,22(9):E1401
[15]	LU Y, JIANG W J, WU X, et al. Peptide T7-modified polypeptide with disulfide bonds for targeted delivery of plasmid DNA for gene therapy of prostate cancer[J]. Int J Nanomedicine,2018,13:6913-6927

Message Board

The C2min aptamer-modified gene delivery system for targeting ADPC/AIPC prostate cancer

doi: 10.3969/j.issn.1006-0111.201906038

Abstract

References

Proportional views

通讯作者: 陈斌, [email protected]

Article Metrics

Related

Proportional views