Determination of glycine in human thrombin by HPLC

SHENG Fengxian; ZHANG Youjie

doi:10.3969/j.issn.1006-0111.201903048

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Objective To establish a HPLC method for glycine assay in the human thrombin. Methods The sample was derivatized with 2,4-dinitrofluorobenzene (DNFB). HPLC was performed on an ODS-C₁₈ column with 50% acetonitrile –0.05mol/L sodium acetate buffer (35:65) as the mobile phase. The flow rate was 1.0 ml/min and detection wavelength at 360 nm. Results The linear range of glycine was 0.006-0.030 mg/ml (r=0.999 6). The average recovery was 100.4%, with RSD 0.44% (n=9). Conclusion This method is simple, accurate and specific. It is suitable for glycine assay in human thrombin.

Determination of glycine in human thrombin by HPLC

Shanghai Xinxing Medicine Co., Ltd, Shanghai 200135, China

Received Date: 2019-03-12

Rev Recd Date: 2019-05-13

Key words:

human thrombin /
glycine /
alanine /
HPLC

Abstract: Objective To establish a HPLC method for glycine assay in the human thrombin. Methods The sample was derivatized with 2,4-dinitrofluorobenzene (DNFB). HPLC was performed on an ODS-C₁₈ column with 50% acetonitrile –0.05mol/L sodium acetate buffer (35:65) as the mobile phase. The flow rate was 1.0 ml/min and detection wavelength at 360 nm. Results The linear range of glycine was 0.006-0.030 mg/ml (r=0.999 6). The average recovery was 100.4%, with RSD 0.44% (n=9). Conclusion This method is simple, accurate and specific. It is suitable for glycine assay in human thrombin.

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Determination of glycine in human thrombin by HPLC

doi: 10.3969/j.issn.1006-0111.201903048

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