LI Yueyue, ZHANG Bin, YUAN Yongfang. Identification of components and metabolites of Saposhnikoviae Radix decoction in rats' plasma by UPLC-Q-TOF-MS[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 512-517. doi: 10.3969/j.issn.1006-0111.2019.06.008
| Citation:
|
LI Yueyue, ZHANG Bin, YUAN Yongfang. Identification of components and metabolites of Saposhnikoviae Radix decoction in rats' plasma by UPLC-Q-TOF-MS[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 512-517. doi: 10.3969/j.issn.1006-0111.2019.06.008
|
Identification of components and metabolites of Saposhnikoviae Radix decoction in rats' plasma by UPLC-Q-TOF-MS
-
Department of Pharmacy, Shanghai Ninth People's Hospital;School of Medicine, Shanghai JiaoTong University, Shanghai 200011, China
- Received Date: 2019-06-15
- Rev Recd Date:
2019-11-05
-
Abstract
Objective To study the prototype components and metabolites of Saposhnikoviae Radixin in rats' plasma. Methods UPLC-Q-TOF-MS method was used to identify the components and metabolites of rat plasma.The Eclipse Plus C18 column(150 mm×2.1 mm,1.8 μm,Agilent)was used.The mobile phase was 0.1% acetic acid solution (A)-0.1% acetic acidacetonitrile (B).The gradient elution was used.The flowing rate was 0.3 ml/min.The injection volume was 3 μl. Results An analytical method for the determination of metabolites of Saposhnikoviae Radix in plasma samples after oral administration.A total of 21 compounds,including 10 prototype components and 11 metabolites,were identified by comparison of their retention time,ion fragmentation information with that of the control plasma,herb extract,and reference compounds. Conclusion The metabolic pathway of the main components of Saposhnikoviae Radixin in rats mainly include hydroxylation,demethylation and glucuronidation,etc.This established method is simple and reliable and provide research basis for revealing the bioactive components of Saposhnikoviae Radix.
-
References
|
[1]
|
OKUYAMA E,HASEGAWA T,MATSUSHITA T,et al.Analgesic components ofSaposhnikovia root (Saposhnikovia divaricata)[J].Chem Pharm Bull,2001,49(2):154-160. |
|
[2]
|
薛宝云,李文,李丽,等.防风色原酮甙类成分的药理活性研究[J].中国中药杂志,2001,25:297-299. |
|
[3]
|
李文,李丽,是元艳,等.防风有效部位的药理作用研究[J].中国实验方剂学杂志,2006,12(6):29-31. |
|
[4]
|
黎建斌,刘丽萍,丘振文.生防风挥发油抗炎止血作用的药理研究[J].新中医,2007,39(8):105-106. |
|
[5]
|
姜艳艳,刘斌,石任兵,等.防风化学成分的分离与结构鉴定[J].药学学报,2007,42(5):505-510. |
|
[6]
|
王松柏,秦雪梅,刘焕蓉,等.防风多糖化学成分的研究[J].化学研究,2008,19(2):66-68. |
|
[7]
|
KANG J,SUN J H,ZHOU L,et al.Characterization of compounds from the roots of Saposhnikovia divaricata by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry[J].Rapid Commun Mass Spectrom,2008,22(12):1899-1911. |
|
[8]
|
CHEN L X,CHEN X Y,SU L,et al.Rapidcharacterisation and identification of compounds in Saposhnikoviae Radix by high-performance liquid chromatography coupled with electrospray ionisation quadrupole time-of-flight mass spectrometry[J].Nat Prod Res,2018,32(8):898-901. |
|
[9]
|
李悦悦,王慧,陈俊,等.RRLC-TOF/MS鉴别防风血浆、尿液中成分及代谢产物[J].第二军医大学学报,2010,31(7):760-763. |
|
[10]
|
LI YY,ZHAO L,ZHANG H,et al.Comparative pharmacokinetics of prim-O-glucosylcimifugin and cimifugin by liquid chromatography-mass spectrometry after oral administration of Radix Saposhnikoviae extract,cimifugin monomer solution and prim-O-glucosylcimifugin monomer solution to rats[J].Biomed Chromatogr,2012,26(10):1234-1240. |
-
-
Proportional views
-
DownLoad: