Effects of letrozole and 45% high-fat diet on induction of rat model of polycystic ovary syndrome with reproductive and metabolic disorder

YIN Ping; LU Lu; CHEN Yuanyuan; LI Li; LI Yun; TONG Guoqing

doi:10.3969/j.issn.1006-0111.2019.05.007

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Objective To establish rat polycystic ovary syndrome model with letrozole combined high fat diet. Methods Eighteen 21-day-old female rats were fed with 45% high-fat diet for 16 weeks.Letrozole was started at the 6th week of age until the end of modeling.Eight rats in control group were fed with regular diet and Saline without letrozole at the 6th week of age.The vaginal smear and ovarian histological changes were investigated.The changes in body mass,organ weight,blood testosterone level,glucose tolerance,blood lipid level,fasting insulin and insulin sensitivity index were measured. Results In the model group,the ovary of the rats showed a multi-cystic change,the granulosa cell layer decreased,and the tunica albuginea increased.Compared with the control group,the body weight of the model group increased,the weight of the ovary and inguinal fat increased,the weight and weight index of the uterus and pituitary gland decreased,the triglyceride and low density lipoprotein increased,the area under the glucose tolerance curve increased,the blood testosterone level increased,the fasting insulin increased,and the insulin sensitivity decreased. Conclusion Letrozole combined with high fat diet could establish an effective PCOS model of abnormal endocrine and metabolic phenotype.

Effects of letrozole and 45% high-fat diet on induction of rat model of polycystic ovary syndrome with reproductive and metabolic disorder

Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200120, China

Received Date: 2018-12-15

Rev Recd Date: 2019-04-22

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Abstract: Objective To establish rat polycystic ovary syndrome model with letrozole combined high fat diet. Methods Eighteen 21-day-old female rats were fed with 45% high-fat diet for 16 weeks.Letrozole was started at the 6th week of age until the end of modeling.Eight rats in control group were fed with regular diet and Saline without letrozole at the 6th week of age.The vaginal smear and ovarian histological changes were investigated.The changes in body mass,organ weight,blood testosterone level,glucose tolerance,blood lipid level,fasting insulin and insulin sensitivity index were measured. Results In the model group,the ovary of the rats showed a multi-cystic change,the granulosa cell layer decreased,and the tunica albuginea increased.Compared with the control group,the body weight of the model group increased,the weight of the ovary and inguinal fat increased,the weight and weight index of the uterus and pituitary gland decreased,the triglyceride and low density lipoprotein increased,the area under the glucose tolerance curve increased,the blood testosterone level increased,the fasting insulin increased,and the insulin sensitivity decreased. Conclusion Letrozole combined with high fat diet could establish an effective PCOS model of abnormal endocrine and metabolic phenotype.

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Reference (19)

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Effects of letrozole and 45% high-fat diet on induction of rat model of polycystic ovary syndrome with reproductive and metabolic disorder

doi: 10.3969/j.issn.1006-0111.2019.05.007

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