Effects of microneedle-roller insertion times on skin permeation characteristics,drug distribution and skin irritation

ZHANG Juanjuan; FU Xiang; ZHU Quangang; ZHANG Lichao

doi:10.3969/j.issn.1006-0111.2019.05.006

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Objective To study the effects of microneedle-roller insertion times on skin permeation. Methods Tretinoin was used as model drug.The in vitro transdermal characteristics in nude mice were studied with Franz diffusion cell and the tissue homogenization method.The drug distribution in nude mice skin was investigated by methylene blue staining and confocal lasers scanning microscope.The skin irritation was evaluated by trans-epidermal water-loss (TEWL) measurement and laser Doppler flowmetry. Results Within the microneedle roller insertion times 1,3,5,8,and 10,the better penetration effect and drug accumulation in skin were achieved with the increment of insertion times.However,there was no significant difference in the skin accumulation between 8 and 10 times of the insertion(P>0.05).The results from methylene blue staining and microscope confocal lasers scanning indicated that the uniformity of needle hole distribution was increased with the increased insertion times.However,some needle hole overlap and skin damages were observed from methylene blue staining when insertion times were more than five.The in vivo drug absorption study indicated that the drug accumulation was inhomogeneous in nude mice skin after one treatment.It was significantly improved after three treatments.The results from TEWL measurements suggested that the recovery time of skin barrier function was 24 hours if insertion was less than five times and 36 hours if more than eight times.The results of laser Doppler flowmetry indicated that the recovery time of skin barrier function was 1 hour if insertion times were less than five,and 2 hours if more than eight times. Conclusion The optimal microneedle insertion time was five times,which could ensure the safety and effectiveness of the skin permeation study in nude mice.

Effects of microneedle-roller insertion times on skin permeation characteristics,drug distribution and skin irritation

1. Department of Pharmacy, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai 200071, China;Department of Pharmacy, Shanghai Pudong New District Xinchang Community Health Service Center, Shanghai 201314, China

2. Department of Pharmacy, Shanghai KongJiang Hospital, Shanghai 200093, China

3. Department of Pharmacy, Shanghai Dermatology Hospital, Shanghai 200443, China

4. Department of Pharmacy, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai 200071, China

Received Date: 2019-03-03

Rev Recd Date: 2019-04-28

Key words:

Abstract: Objective To study the effects of microneedle-roller insertion times on skin permeation. Methods Tretinoin was used as model drug.The in vitro transdermal characteristics in nude mice were studied with Franz diffusion cell and the tissue homogenization method.The drug distribution in nude mice skin was investigated by methylene blue staining and confocal lasers scanning microscope.The skin irritation was evaluated by trans-epidermal water-loss (TEWL) measurement and laser Doppler flowmetry. Results Within the microneedle roller insertion times 1,3,5,8,and 10,the better penetration effect and drug accumulation in skin were achieved with the increment of insertion times.However,there was no significant difference in the skin accumulation between 8 and 10 times of the insertion(P>0.05).The results from methylene blue staining and microscope confocal lasers scanning indicated that the uniformity of needle hole distribution was increased with the increased insertion times.However,some needle hole overlap and skin damages were observed from methylene blue staining when insertion times were more than five.The in vivo drug absorption study indicated that the drug accumulation was inhomogeneous in nude mice skin after one treatment.It was significantly improved after three treatments.The results from TEWL measurements suggested that the recovery time of skin barrier function was 24 hours if insertion was less than five times and 36 hours if more than eight times.The results of laser Doppler flowmetry indicated that the recovery time of skin barrier function was 1 hour if insertion times were less than five,and 2 hours if more than eight times. Conclusion The optimal microneedle insertion time was five times,which could ensure the safety and effectiveness of the skin permeation study in nude mice.

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Reference (11)

[1]	占浩慧,黄颖聪,马凤森.微针穿刺性能评价方法研究进展[J].中国药学杂志,2018,53(22):1890-1895.
[2]	李雅,张娟娟,张立超,等.改装滚轮微针促进人增生性瘢痕皮肤对醋酸曲安奈德的吸收[J].第二军医大学学报,2015,36(1):58-64.
[3]	张娟娟,张立超,朱全刚,等.改装滚轮微针用于醋酸曲安奈德经皮给药的特性试验[J].中国医院药学杂志,2015,35(4):292-296.
[4]	ZHOUC P,LIU Y L,WANG H L,et al.Transdermal delivery of insulin using microneedle rollers in vivo[J].Int J Pharm,2010,392(1-2):127-133.
[5]	YOONJ,PARK D,SON T,et al.A physical method to enhance transdermal delivery of a tissue optical clearing agent:combination of microneedling and sonophoresis[J].Lasers Surg Med,2010,42(5):412-417.
[6]	BADRAN M M,KUNTSCHE J,FAHR A.Skin penetration enhancement by a microneedle device (Dermaroller) in vitro:dependency on needle size and applied formulation[J].Eur J Pharm Sci,2009,36(4-5):511-523.
[7]	YOUS K,NOH Y W,PARK H H,et al.Effect of applying modes of the polymer microneedle-roller on the permeation of L-ascorbic acid in rats[J].J Drug Target,2010,18(1):15-20.
[8]	张娟娟,朱全刚,张立超,等.不同密度滚轮微针对维A酸经皮渗透的影响[J].药学服务与研究,2013,13(6):440-444.
[9]	张娟娟.力度可控性滚轮微针的构建及其对大面积皮肤药物吸收影响研究[D].宁夏:宁夏医科大学,2014.
[10]	鞠强.维A酸类药物在痤疮治疗中的应用[J].皮肤病与性病,2018,40(1):26-28.
[11]	WANG Q,JAIMES-LIZCANO Y A,LAWSON L B,et al.Improved dermal delivery of FITC-BSA using a combination of passive and active methods[J].J Pharm Sci,2011,100(11):4804-4814.

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Effects of microneedle-roller insertion times on skin permeation characteristics,drug distribution and skin irritation

doi: 10.3969/j.issn.1006-0111.2019.05.006

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